癌变·畸变·突变 ›› 2005, Vol. 17 ›› Issue (1): 27-29.doi: 10.3969/j.issn.1004-616x.2005.01.009

• 论著 • 上一篇    下一篇

卤代物对盐藻生长抑制实验的联合效应的观察

黄小娟;沈洛夫;姜建国;赖凤英   

  1. 华南理工大学食品与生物工程学院, 广东 广州 510640
  • 收稿日期:2004-05-17 修回日期:2004-08-10 出版日期:2005-01-30 发布日期:2005-01-30
  • 通讯作者: 黄小娟

Test of Combined-toxicity of Halids to Dunaliella Salina

HUANG Xiao-juan;SHEN Luo-fu;JIANG Jian-guo;et al   

  1. College of Food and Biological Engineering,South China University of Technology,Guangzhou 510640, China
  • Received:2004-05-17 Revised:2004-08-10 Online:2005-01-30 Published:2005-01-30
  • Contact: HUANG Xiao-juan

摘要: 背景与目的: 根据国际经济合作与发展组织(OECD)藻类生长抑制实验的国际标准方法,研究了卤代物对盐藻的毒性作用。 材料与方法: CCl4、CHCl3和CHBr3两两混合共3个浓度组、三者混合1个浓度组和1个对照组,毒物浓度设置参照国家GB5749-1985《生活饮用水卫生标准》,对盐藻进行培养,48 h后进行平板计数。 结果: 得到了各试验组对盐藻(Dunaliella salina)的48 h半数有效抑制浓度EC50值,以及得到各混合物毒性相加和S与联合效应相加指数AI,由于AI值均在-0.1~0.1,表明混合物对盐藻的毒性具有相加作用。 结论: 盐藻作为受试生物在毒物的评价方面具有广阔的前景。

关键词: 盐藻, 联合毒性, 卤代物

Abstract: BACKGROUND & AIM: According to the standard methods of the Organization for Economic Co-operation and Development (OECD) for algal growth inhibition test, the combined-toxicitiy test of three kinds of halid-mixtures to Dunaliella salina was conducted. MATERIAL AND METHODS: Four groups of the tests were set by combining any two of CHCl3, CCl4, CHBr3 and all the three compounds, another group was a control. Concentrations were set according to the National drinking water health standard (GB5749-1985) and D. salina was cultivated in these groups. Cell counting was conducted after 48 h. RESULTS: The EC50 of each group was obtained, and the combined-toxicity's addition-indexes (AI) of the groups were all among -0.1~0.1, which indicated that the toxicity of these compounds to D.salina was additive effect. CONCLUSION: As a tested organism, D. salina has a wide applied potential in the area of toxicology.

Key words: Dunaliella salina, combined toxicity, halids