癌变·畸变·突变 ›› 2006, Vol. 18 ›› Issue (6): 469-471.doi: 10.3969/j.issn.1004-616x.2006.06.015

• 论著 • 上一篇    下一篇

壬基酚对牡蛎血淋巴细胞的DNA损伤

李正炎 卫东   

  1. 中国海洋大学海洋环境与生态教育部重点实验室；中国海洋大学环境科学与工程学院
  • 收稿日期:2005-09-05 修回日期:2006-01-16 出版日期:2006-11-30 发布日期:2006-11-30
  • 通讯作者: 李正炎

DNA Damage Caused by Nonylphenol in Oyster Hemolymph Cells

LI Zheng-yan, WEI Dong   

  1. (1.Key Lab of Marine Environmental Science and Ecology,Ministry of Education,Ocean University of China,Qingdao 266003，Shandong;2.Faculty of Environmental Science ＆ Engineering, Ocean University of China, Qingdao 266003，Shandong,China)
  • Received:2005-09-05 Revised:2006-01-16 Online:2006-11-30 Published:2006-11-30
  • Contact: LI Zheng-yan

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摘要： 【摘要】 背景与目的： 采用单细胞凝胶电泳技术研究壬基酚(nonylphenol, NP)对牡蛎血淋巴细胞DNA的损伤作用。 材料与方法：分别以0(阴性对照)、0.025、0.10、0.25和1.00 mg/L的NP处理牡蛎24 h和48 h,检测NP对血淋巴细胞DNA的损伤作用。 结果： 当NP在0.025 mg/L时可以使牡蛎血淋巴细胞产生轻微的DNA损伤, 染毒24 h后DNA破坏率为6％，48 h后破坏率为20％。随着NP浓度的增加和染毒时间的延长，血淋巴细胞的DNA破坏率相应增加,其中最高浓度的处理组(1.00 mg/L)DNA 损伤最为明显, 染毒24 h后的破坏率为30％，48 h后为60％。 结论： NP对牡蛎血淋巴细胞具有遗传毒性。

关键词: 单细胞凝胶电泳, 壬基酚, 牡蛎, DNA损伤, 遗传毒性, 血淋巴细胞

Abstract: 【ABSTRACT】 BACKGROUND ＆ AIM：The effect of nonylphenol(NP) on DNA damage in oyster hemolymph cells was measured by single-cell gel electrophoresis assay in vivo. MATERIAL AND METHODS： Oysters were exposed to various NP concentrations (0, 0.025, 0.10, 0.25 and 1.00 mg/L) and DNA damage in hemolymph cells was assessed after 24 h and 48 h. RESULTS: NP at 0.025 mg/L caused slight damage to cellular DNA. The percentage of cellular damage was 6％ and 20％ after 24 h and 48 h, respectively. NP at 1.00 mg/L caused serious damage to cellular DNA. The percentage of cellular damage was 30％ and 60％ after 24 h and 48 h, respectively. CONCLUSION: NP causes genetic toxicity to oyster hemolymph cells.

Key words: single-cell gel electrophoresis, nonylphenol, oyster, DNA damage, genetic toxicity, hemolymph cell

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