关键词: 微核试验, 熟练程度, 染色时间, 阅片时间

Abstract: BACKGROUND ＆ AIM：To study the effect on MN frequency identification caused by different skill level, staining time and scoring time. MATERIALS AND METHODS: A blood sample was collected from a healthy 30_year_old male. Lymphocytes was isolated and then cultured in RPMI medium in triplicates. Two of the 3 cultured groups were then exposed to 1Gy or 2 Gy gamma_rays and the other one was control. Parallel scoring spots (a and b) in same slide was made according to CBMN assay method, 2 simultaneously parepared slides of each group was stained by Giemsa at 2 different times，and then 6 scorers with different skill levels scored the MN frequency. RESULTS: Variation of MN frequency scored by scorers with different skill levels was not significant.The MN frequency scoring for spot a and b at different times showed no significant difference among different scorers. The observed endpoints, MNed、MNi、NPB and NDI, stained at different times, were not different significantly either. CONCLUSION: Skill level, staining time or scoring time did not show significant interference on estimation of the dose_effect relationship between MN frequency and radiation dose.

Key words: micronucleus test, skill level, staining time, scoring time