关键词: 葡多酚, 乳腺癌, 雌激素受体, 增殖活性

Abstract: BACKGROUND AND AIM: To study the effect of grape procyanidins(GPC) on the expression of estrogen receptor in breast cancer cells. MATERIALS AND METHODS: The MCF_7 cells were cultured with different concentrations of GPC. The MCF_7 cells were divided into four groups: 200,100,10 μg/ml GPC groups and control group. The expressions of ER and Bcl_2 protein were assessed by immunohistochemistry. The proliferation activity of breast cancer cells was evaluated by MTT assay. RESULTS: The expression of ER protein in 100 and 200 μg/ml GPC groups were(85.02±1.53)％ and(73.14±1.05)％, respectively. The proliferative activity of the cells were 0.365±0.009 and 0.283±0.008, respectively. Compared with control group, all the results mentioned were significantly decreased (P<0.05). CONCLUSION: GPC could inhibit the expression of ER protein and proliferative activity of breast cancer cells.

Key words: grape procyanidins, breast cancer, estrogen receptor, proliferative activity