子宫内膜癌中p16甲基化、Her-2表达及血清CA125水平与临床病理特征的关系

doi:10.3969/j.issn.1004-616x.2009.02.015

癌变·畸变·突变 ›› 2009, Vol. 21 ›› Issue (2): 141-144.doi: 10.3969/j.issn.1004-616x.2009.02.015

子宫内膜癌中p16甲基化、Her-2表达及血清CA125水平与临床病理特征的关系

周　莉,黄　萍,朱安娜

汕头大学医学院附属肿瘤医院妇科，广东　汕头　505031

收稿日期:2008-09-27 修回日期:2008-11-11 出版日期:2009-03-30 发布日期:2009-03-30

Relationships between Methylation of p16 Gene，Her-2 Expression and Serum CA125 Level with Clinicopathological Characteristics in Endometrial Carcinoma

ZHOU Li， HUANG Ping， ZHU An-na

Department of Gynecologic Surgery, Affiliated Tumor Hospital, Shantou University Medical College, Shantou 515031, China

Received:2008-09-27 Revised:2008-11-11 Online:2009-03-30 Published:2009-03-30

摘要/Abstract

摘要： 背景与目的：研究子宫内膜癌(endometrial carcinoma， EC)组织中抑癌基因p16的甲基化和癌基因Her-2的表达及血清肿瘤标志物CA125水平及其与临床病理特征间的关系。材料与方法：收集手术切除标本EC组织38例及内膜对照组织20例，采用甲基化特异性PCR法检测p16的甲基化状态；用免疫组化法检测Her-2蛋白的表达；对所有病例术前均采用免疫放射法测定血清CA125水平，并分析3者与临床病理特征之间的关系。结果： 20例对照组织中p16的甲基化、Her-2表达、血清CA125均阴性(0/20)，而38例EC组织中3者阳性率分别为57.9％ (22/38)、60.5％(23/38)、23.7％(9/38)，与对照组比较差异均有统计学意义(P均<0.05)。p16甲基化、Her-2表达的阳性率以及血清CA125阳性率均与EC临床分期有相关关系(P均<0.05)，p16甲基化和血清CA125阳性率与病理分化有相关关系(P均<0.05), 血清CA125水平与宫外淋巴结转移有关(P<0.05)，3者与肿瘤浸润深度均显著相关(P均<0.05)。Spearman相关分析结果显示Her-2与CA125之间呈正相关关系(rs=0.323, P=0.048),与p16甲基化之间呈负相关关(rs=0.362,P=0.026)。结论： p16的甲基化、Her-2表达、血清CA125的阳性率反映了肿瘤不同阶段中各基因及肿瘤标志物的不同表现，联合检测对指导术后治疗具有一定的意义。

关键词: 子宫内膜癌, p16, Her-2, CA125, 甲基化

Abstract: BACKGROUND AND AIM: To study the correlations between p16 gene methylation,Her-2 expression and serum CA125 level in endometrial carcinoma(EC)and clinicopathological characteristics. MATERIALS AND METHODS: A total of 38 EC tissues and 20 normal controls (NC) were analyzed.We studied the p16 gene methylation by methylation specific PCR (MSP)and Her-2 expression by Envision method, and the preoperative serum CA125 levels were checked by radio-immunity in all cases. The correlations of expression levels and EC clinicopathological characteristic were studied. RESULTS: The positive rates of p16 methylation , Her-2 expression and serum CA125 level were 57.9％(22/38),60.5％(23/38)and 23.7％(9/38),respectively, in 38 cases of EC, but were not detected in 20 normal controls, the difference was significant (P<0.05). Methylation of p16 gene, Her-2 expression and serum CA125 level were all statistically related to clinical stage of EC in test group(P<0.05), methylation of p16 gene and the level was statistically related to histological grade(P<0.05). Serum CA125 levels were statistically related to pelvic lymph node mestastasis(P<0.05),the 3 indexes were all statistically related to the depth of tumor invasion(P<0.05). Spearman correlation revealed there was a statistically positive relationship between Her-2 and serum CA125 level(P=0.048)and negative correlation between methylation of p16 gene and Her-2 expression(P=0.026). CONCLUSION: The measurements of p16 methylation, Her-2 expression and serum CA125 level may reflect different behaviors of genes and tumor markers at different stages of tumor pathogenesis. They may be useful tools for providing information about the malignant degree,prognosis, and may guide postoperative treatment for patients with EC.

Key words: endometrial carcinoma, p16, Her-2, CA125, methylation

周　莉, 黄　萍, 朱安娜. 子宫内膜癌中p16甲基化、Her-2表达及血清CA125水平与临床病理特征的关系[J]. 癌变·畸变·突变, 2009, 21(2): 141-144.

ZHOU Li, HUANG Ping, ZHU An-na. Relationships between Methylation of p16 Gene，Her-2 Expression and Serum CA125 Level with Clinicopathological Characteristics in Endometrial Carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2009, 21(2): 141-144.

[1]	王一帆, 许铭炎, 邓小玲. RNA的N⁶-甲基腺嘌呤甲基化在肿瘤发生中作用的研究进展[J]. 癌变·畸变·突变, 2021, 33(1): 77-80.
[2]	尹璐, 马成斌, 刘平, 张祎, 赵学军. P16/Ki-67双染联合DNA倍体分析在ASCUS分流诊断中的应用价值[J]. 癌变·畸变·突变, 2020, 32(4): 264-268.
[3]	樊琼玲, 王家威, 俞金秀, 马正文, 张石蕾, 蒋红, 由淑萍. ECE-1基因去甲基化在腹主动脉缩窄术后左室肥厚大鼠中的作用及机制研究[J]. 癌变·畸变·突变, 2020, 32(1): 8-14.
[4]	田雪蕾, 封江彬, 田梅, 刘青杰. 甲基化芯片检测电离辐射诱导人外周血淋巴细胞DNA甲基化水平的变化[J]. 癌变·畸变·突变, 2019, 31(6): 434-439.
[5]	彭长凤, 谢杏, 柯跃斌. 纳米氧化石墨烯诱导人类支气管上皮细胞DNA氧化与甲基化的实验研究[J]. 癌变·畸变·突变, 2019, 31(6): 454-458,463.
[6]	章梦莹, 王博深, 张虹, 浦跃朴, 尹立红, 张娟. G6PD缺陷对1,4-苯醌致K562细胞DNA甲基化的影响及其机制[J]. 癌变·畸变·突变, 2019, 31(3): 186-192.
[7]	吴德生, 杨飞, 袁建辉, 杨淋清, 黄海燕, 刘建军, 庄志雄. PM2.5对斑马鱼胚胎发育早期氧化应激和DNA甲基化调控相关基因表达的影响[J]. 癌变·畸变·突变, 2017, 29(5): 379-382.
[8]	王治, 凌曦, 张国伟, 高建芳, 邹鹏, 井澈, 林哲韬, 刘晋祎, 曹佳, 敖琳. 叶酸对1,3-丁二烯诱发的小鼠DNA低甲基化和染色体损伤的影响[J]. 癌变·畸变·突变, 2017, 29(3): 189-193.
[9]	张海燕, 黎青叶, 陈燊, 陈雯, 何志妮. 环境化学污染物暴露相关基因甲基化焦磷酸测序方法的构建和应用[J]. 癌变·畸变·突变, 2017, 29(2): 81-86.
[10]	刘海龙, 高玮, 古盼, 邓燕霞, 黄新凤, 吴德生, 刘建军, 黄海燕. BaP诱导细胞恶性转化过程中DNA甲基化水平的变化[J]. 癌变·畸变·突变, 2017, 29(1): 42-45,50.
[11]	Bernd Kaina. 甲基化抗肿瘤药物治疗中的DNA修复:治疗效果与健康结局[J]. 癌变·畸变·突变, 2016, 28(5): 333-341,347.
[12]	赖怡, 陈佳红, 张航, 乐聪, 姜岩, 陈涛. 丙烯酰胺对F344大鼠肝毒性的分子机制研究[J]. 癌变·畸变·突变, 2016, 28(3): 174-178.
[13]	魏绍峰, 张爱华. DNA低甲基化及其与肿瘤关系的研究进展[J]. 癌变·畸变·突变, 2016, 28(3): 243-245,249.
[14]	程琳, 孙平楠, 谢庆东, 周小玲. 预扩增qPCR方法检测少量小鼠早期胚胎细胞中DNA甲基化相关基因的表达[J]. 癌变·畸变·突变, 2016, 28(1): 51-55.
[15]	王全凯, 谢广云, 刘红梅, 许建宁. 甲基丙烯酸环氧丙酯致16HBE细胞恶性转化不同时期METTL9基因甲基化状态分析[J]. 癌变·畸变·突变, 2015, 27(6): 432-436.

子宫内膜癌中p16甲基化、Her-2表达及血清CA125水平与临床病理特征的关系

Relationships between Methylation of p16 Gene，Her-2 Expression and Serum CA125 Level with Clinicopathological Characteristics in Endometrial Carcinoma

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价