癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (4): 261-264.doi: 10.3969/j.issn.1004-616x.2010.04.003

• 论著 • 上一篇    下一篇

两种食管鳞癌细胞系增殖和侵袭能力的比较研究

谢仰民1;董辉2;＃;谢剑君2;吴智勇2;杜则澎3;李利艳3;许秀娥3;周飞1;陈涛2;石宏顺3;许丽艳3;李恩民2   

  1. 1. 汕头大学医学院实验动物中心； 2. 汕头大学医学院生物化学与分子生物学教研室；3. 汕头大学医学院肿瘤病理研究室， 广东 汕头 515041
  • 收稿日期:2010-03-31 修回日期:2010-05-04 出版日期:2010-07-30 发布日期:2010-07-30
  • 通讯作者: 李恩民

Comparative study on the proliferation and invasion of two esophageal squamous carcinoma cell lines

XIE Yang-min1;DONG Hui2;＃;XIE Jian-jun2;WU Zhi-yong2;DU Ze-peng3;LI Li-yan3;XU Xiu-e3; ZHOU Fei1; CHEN Tao2;SHI Hong-shun3;XU Li-yan3;LI En-min2   

  1. 1. Department of Experimental Animal Center, Medical College of Shantou University; 2. Department of Biochemistry and Molecular Biology, Medical College of Shantou University; 3. Department of Pathology, The Key Immunopathology Laboratory of Guangdong Province, Medical College of Shantou University, Shantou 515041
  • Received:2010-03-31 Revised:2010-05-04 Online:2010-07-30 Published:2010-07-30
  • Contact: LI En-min

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摘要： 目的： 研究两种来源不同的食管鳞癌细胞系EC109和KYSE510分裂增殖能力和侵袭能力的差异。 方法： 利用MTT实验和侵袭实验，在体外分别检测EC109和KYSE510细胞的分裂增殖能力和侵袭能力；采用裸鼠皮下接种实验和爪垫皮下接种淋巴结转移模型，比较两种细胞在体内的成瘤能力、局部侵袭能力和区域淋巴结转移能力的差异；免疫印迹检测上皮-间充质细胞转换标志蛋白E-cadherin、γ-cadherin和Vimentin在两种细胞中的表达水平。 结果： 体外实验表明，EC109细胞的分裂增殖和侵袭能力均明显较KYSE510细胞的强(P<0.05)；皮下接种和爪垫皮下接种淋巴结转移实验显示EC109细胞的成瘤能力、局部侵袭能力和淋巴结转移能力均较KYSE510细胞的高；免疫印迹检测发现，E-cadherin和γ-cadherin在EC109细胞中的表达水平较KYSE510细胞中的表达低，而Vimentin在EC109细胞中的表达则较KYSE510细胞中的表达高，提示EC109细胞发生上皮-间充质细胞转换的程度较KYSE510的高。 结论： EC109细胞的增殖和侵袭能力均较KYSE510细胞的强，上皮-间充质细胞转换可能是导致这种差异的原因之一。

关键词: 食管癌细胞, 细胞增殖, 细胞侵袭, 转移

Abstract: OBJECTIVE: This study investigated the proliferation and invasiveness of two different esophageal squamous carcinoma (ESCC) cell lines, EC109 cells and KYSE510 cells. METHODS: MTT assay and invasiveness assay were used to explore the proliferation and invasiveness of EC109 cells and KYSE510 cells in vitro. Then, tumor formation assay in the nude mice was employed to analyze the tumorigenesis and footpad tumor model was used to assess the metastasis in lymph nodes of the two cell lines. Finally, the expressions of E-cadherin, γ-cadherin and Vimentin were detected to estimate the involvement of epithelial-mesenchymal transition (EMT). RESULTS: In vitro experiments showed that EC109 cells proliferated faster than KYSE510 cells and the invasiveness of EC109 cells was higher than that of KYSE510 cells. The tumorigenesis of EC109 cells was greater than KYSE510 and the metastasis in lymph nodes was found more frequently in EC109 cells. Furthermore, expressions of E-cadherin and γ-cadherin were down-regulated in EC109 cells as compared to KYSE510 cells, while the level of Vimentin was up-regulated. CONCLUSION: EC109 was a ESCC cell line with high potential of proliferation and invasiveness and KYSE510 was not. The difference in proliferation and invasiveness between these two cell lines might be mediated by EMT.

Key words: esophageal squamous carcinoma cells, cell proliferation, cell invasiveness

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