淮安食管鳞癌与癌旁组织中差异表达microRNAs的初步分析

doi:10.3969/j.issn.1004-616x.2010.06.002

癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (6): 418-422.doi: 10.3969/j.issn.1004-616x.2010.06.002

淮安食管鳞癌与癌旁组织中差异表达microRNAs的初步分析

刘　辉1，刘　冉1，苏耀耀1，杨　淼1，浦跃朴1，尹立红1,王　仪2，潘恩春2，郭　伟3，尤振兵3

1.东南大学公共卫生学院环境医学工程教育部重点实验室，南京 210009；2. 江苏省淮安市疾病预防控制中心，淮安 223001；3. 江苏省淮安市第一人民医院，淮安 223000

收稿日期:2010-06-08 修回日期:2010-10-13 出版日期:2010-11-30 发布日期:2010-11-30
通讯作者: 尹立红

A pilot study of differentially expressed microRNAs between esophageal squamous cell carcinoma and adjacent tissues in Huai'an population

LIU Hui1, LIU Ran1, SU Yao-yao1,YANG Miao1,PU Yue-pu1,YIN Li-hong1,,WANG Yi2,PAN En-chun2,GUO Wei3,YOU Zhen-bin3

1. Key Laboratory of Environmental Medical Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009; 2. Huai'an Center for Disease Control and Prevention, Huai'an 223001; 3. Huai'an First People's Hospital, Huai'an 223000,Jiangsu, China

Received:2010-06-08 Revised:2010-10-13 Online:2010-11-30 Published:2010-11-30
Contact: YIN Li-hong

摘要/Abstract

摘要： 目的：初步筛选淮安食管鳞癌与癌旁组织中差异表达的microRNAs，为进一步研究与肿瘤相关的microRNA在食管鳞癌发病机制中的作用奠定基础。方法：选取3例食管鳞癌病人癌组织及其癌旁组织为样本，采用AgilentTM高通量的microRNA微阵列芯片检测miRNAs的表达水平，应用配对t检验分析芯片数据，筛选食管鳞癌组织和癌旁组织中差异表达的内源性microRNAs。结果： MicroRNA微阵列芯片检测的866个人类相关microRNA探针中，食管鳞癌组织与配对癌旁组织样本表达差异有统计学意义的microRNAs有15个 (P<0.05)：其中表达上调的有7个，分别为hsa_miR_20a， hsa_miR_155，hsa_miR_574_5p，hsa_miR_21，hsa_miR_183，hsa_miR_601，hsa_miR_623；表达下调的有8个，分别为hsa_miR_186，hsa_miR_10a，hsa_miR_144，hsa_miR_338_3p，hsa_miR_192，hsa_miR_218，hsa_miR_451，hsa_miR_139_5p。结论：在食管鳞癌组织和癌旁组织中初步筛选了15个差异表达的microRNAs，它们可能与食管鳞癌的发生和发展相关。

关键词: 食管鳞癌, microRNA, 差异表达, 微阵列

Abstract: OBJECTIVE: To study the difference of microRNA expression profiles between esophageal squamous cell carcinoma(ESCC) tissues and adjacent tissues in Huai'an．The result of this study may provide theoretical support and experimental basis for further research on mechanism of microRNAs in the pathogenesis of esophageal squamous cell carcinoma. METHODS: Cancer tissues and corresponding normal esophageal tissues(NET) adjacent to tumor from 3 patients with newly diagnosed ESCC were obtained. Endogenous microRNAs were determined by high_throughput microRNA microarray．RESULTS: Among the 866 human relevant microRNA probes detected by microRNA microarray, the difference of fifteen microRNAs between esophageal cancer tissues and tissues adjacent to the tumors was statistically significant(P<0.05), including a set of 7 overexpressed microRNAs(hsa_miR_20a, hsa_miR_155, hsa_miR_574_5p, hsa_miR_21, hsa_miR_183, hsa_miR_601, hsa_miR_623) and 8 under_expressed microRNAs(hsa_miR_186, hsa_miR_10a, hsa_miR_144, hsa_miR_338_3p, hsa_miR_192, hsa_miR_218, hsa_miR_451, hsa_miR_139_5p). CONCLUSION: There was a special profile of differentially expressed microRNAs between ESCC and NET, which may be associated with the occurrence and development of esophageal squamous cell carcinoma.

Key words: esophageal squamous cell carcinoma, microRNA, differential expression, microarray

刘　辉, 刘　冉, 苏耀耀, 杨　淼, 浦跃朴, 尹立红, 王　仪, 潘恩春, 郭　伟, 尤振兵. 淮安食管鳞癌与癌旁组织中差异表达microRNAs的初步分析[J]. 癌变·畸变·突变, 2010, 22(6): 418-422.

LIU Hui, LIU Ran, SU Yao-yao, YANG Miao, PU Yue-pu, YIN Li-hong, , WANG Yi, PAN En-chun, GUO Wei, YOU Zhen-bin. A pilot study of differentially expressed microRNAs between esophageal squamous cell carcinoma and adjacent tissues in Huai'an population[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(6): 418-422.

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淮安食管鳞癌与癌旁组织中差异表达microRNAs的初步分析

A pilot study of differentially expressed microRNAs between esophageal squamous cell carcinoma and adjacent tissues in Huai'an population

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