›› 2011, Vol. 23 ›› Issue (3): 219-221.doi: 10.3969/j.issn.1004-616x.2011.03.015

• 检测研究 • 上一篇    下一篇

排毒清火凉茶粉的急性毒性和致突变性实验研究

王 兰1,张永东1,郭红云1,张晓文2，*,赵广才2,王 玉3   

  1. 1.甘肃省医科院药理毒理实验中心，甘肃 兰州 730050；2.广州市体育科学研究所，广东 广州 510650；3. 兰州大学公共卫生学院，甘肃 兰州 730000
  • 收稿日期:2010-09-13 修回日期:2011-02-24 出版日期:2011-05-30 发布日期:2011-05-30
  • 通讯作者: 张晓文

Study of toxicology on Paiduqinghuo herbal tea powder

WANG Lan1，ZHANG Yongdong1，GUO Hongyun1，ZHANG Xiaowen2，*，ZHAO Guangcai2，WANG Yu3   

  1. 1. Center of Pharmacology and Toxicology, Gansu Province Academy of Medical Sciences, Lanzhou 730050, Gansu; 2. Department of Sports Nutrition,Guangzhou Institute of Sports Science, Guangzhou 510650，Guangdong; 3. College ofPublic Health, Lanzhou University, Lanzhou 730000, Gansu, China
  • Received:2010-09-13 Revised:2011-02-24 Online:2011-05-30 Published:2011-05-30
  • Contact: ZHANG Xiao-wen

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摘要： 研究排毒清火凉茶粉的急性经口毒性和致突变性，对其安全性作出评价。 方法： 采用最大耐受剂量法观察动物急性毒性反应和死亡情况，测定受试物最大耐受量 (MTD)。采用Ames试验、小鼠精子畸变试验和骨髓细胞微核试验测定排毒清火凉茶粉的致突变性。 结果： 小鼠对排毒清火凉茶粉的最大耐受量为36.0 g/kg，大鼠的最大耐受量为18.0 g/kg。Ames试验结果显示，凉茶粉5个剂量(每皿分别为100、500、1 000、2 500、5 000 μg ) 下的TA97、TA98、TA100、TA102菌株回变菌落数与阴性对照比较，差异均无统计学意义 (P均>0.05)。小鼠精子畸变试验显示，凉茶粉各剂量组(3、6、12 g/kg)小鼠精子畸形率在1.75%～1.92%，与阴性对照组 (1.72%)比较，差别无统计学意义 (P>0.05)。小鼠骨髓微核试验显示，凉茶粉各剂量组(3、6、12 g/kg)小鼠骨髓嗜多染红细胞微核率为0.4‰～1‰，与阴性对照组 (0.6‰～0.8‰)比较，差别无统计学意义 (P>0.05)。 结论： 排毒清火凉茶粉属实际无毒物质。在本实验条件下，未显示其有致突变作用。

关键词: 凉茶, 急性毒性, 致突变性, 安全性评价

Abstract: To investigate the acute toxicity and mutagenicity test of Paiduqinghuo herbal tea powder and evaluate its safety. METHODS： The acute toxicity and mortality were observed，maximum tolerated dose was determined by using the maximum tolerated dose method. The mutagenicity was observed by Ames test，mouse sperm abnormality test， bone marrow cell micronucleus test, respectively. RESULTS： The maximum tolerated dose are 36 g/kg and 18 g/kg. Ames test results showed that using 5 doses of Paiduqinghuo herbal tea powder(100，500，1 000，2 500， 5 000 μg/plate，respectively)，the number of revertant colonies of TA97，TA98， TA100，TA102 strains were not significantly different compared with negative control group. Mouse sperm abnormality test results showed that the rate in herbal tea powder groups(3，6，12 g/kg，respectively) were 1.75%-1.92%，which had no significant difference compared with the control group(1.95%) (P > 0.05). Mouse bone marrow micronucleus test results showed that the micronucleus rate in herbal tea powder groups(3，6，12 g/kg，respectively) were 0.4‰-1‰， which had no significant difference compared with control group (0.6 ‰-0.8 ‰) (P > 0.05). CONCLUSION： Paiduqinghuo herbal tea powder is a non-toxic substance，under these experimental conditions，there was no mutagenic effect.

Key words: herbal tea, acute toxicity, mutagenicity, safety evaluation

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