›› 2011, Vol. 23 ›› Issue (5): 367-369.doi: 10.3969/j.issn.1004-616x.2011.05.010

• 论著 • 上一篇    下一篇

宝藿苷-I联合5-FU 对食管癌Eca-109细胞增殖及凋亡的影响

刘晓霞,郝玉拴,李菊梅,刘冬青,陈剑华,姚艳冰   

  1. 1. 河北工程大学医学院，河北 邯郸 056029；2. 河北工程大学临床医学院，河北 邯郸 056029；3. 邯郸市第一医院，河北 邯郸 056029
  • 收稿日期:2011-04-20 修回日期:2011-07-06 出版日期:2011-09-30 发布日期:2011-09-30
  • 通讯作者: 刘晓霞

Effects of Baohuoside-I and 5-FU on the proliferation and apoptosis of human esophageal carcinoma cell Eca-109

LIU Xiao-xia，HAO Yu-shuan，LI Ju-mei，LIU Dong-qing，CHEN Jian-hua，YAO Yan-bing   

  1. 1. Medical College of Hebei University of Engineering, Handan 056029; 2. Clinical Medical College of Hebei University of Engineering, Handan 056029; 3. Handan First Hospital, Handan 056029, Hebei, China
  • Received:2011-04-20 Revised:2011-07-06 Online:2011-09-30 Published:2011-09-30
  • Contact: LIU Xiao-xia

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摘要： 目的： 研究宝霍苷-Ⅰ与5-FU联合应用对食管癌Eca-109细胞增殖和凋亡的影响。方法：实验设联合用药组 (12.5 mg/L宝霍苷-Ⅰ+12.5 mg/L 5-FU)、阴性对照组 (0.1% DMSO)、25 mg/L宝藿苷-I组和12.5 mg/L 5-FU组，共4组，每组均设3个复孔，每孔100 μl (含Eca-109细胞数为1×104个)。作用24、48、72 h时，采用四甲基偶氮唑盐 (MTT)法分别检测各组Eca-109细胞的增殖；并在作用48 h后，用流式细胞术检测Eca-109细胞凋亡率；用Western blot技术检测Bcl-2、Bax蛋白的表达。结果：宝霍苷-Ⅰ、5-FU单用及其联合应用在24、48和72 h时均可明显抑制Eca-109细胞的增殖，与阴性对照组比较，差异均有统计学意义(P 均<0.05)，联合应用的抑制作用显著优于单独应用 (P <0.05)。宝霍苷-Ⅰ、5-FU单独及其联合作用48 h后，均可诱导Eca-109细胞凋亡，均可下调Bcl-2蛋白的表达，上调Bax蛋白的表达，与对照组相比差异均有统计学意义 (P均<0.05)，且联合用药组较单独用药组作用明显 (P <0.05)。结论：宝霍苷-Ⅰ与5-FU联合应用对Eca-109细胞的增殖抑制和凋亡诱导有协同作用，可能与下调Bcl-2蛋白的表达、上调Bax蛋白的表达有关。

关键词: 宝霍苷-Ⅰ, 5-FU, 增殖, 细胞凋亡, Bcl-2, Bax, 食管癌

Abstract: OBJECTIVE: To study the effects of baohuoside-I in combination with 5-FU on the proliferation and apoptosis of human esophageal carcinoma cell Eca-109. METHODS：The cells were divided into 4 groups： combination group (12.5 mg/L baohuoside-I and 12.5 mg/L 5-FU)，negative control group (0.1% DMSO)，25 mg/L baohuoside-I group and 12.5 mg/L 5-FU group，with 3 holes in each group and 100 μl each hole (1×104 cells ). After treatment for 24，48，72 h，the inhibitory effect on proliferation of Eca-109 cells was analyzed by MTT. After treatment for 48 h，apoptosis of Eca-109 cells was measured with flow cytometry；the expression of Bcl-2 protein and Bax protein of Eca-109 cells was measured with Western blot. RESULTS：Baohuoside-I，5-FU alone and baohuoside-I in combination with 5-FU inhibited proliferation of Eca-109 cells (P all <0.05)，the inhibitory effect of the combination group was superior to either alone (P <0.05). After treatment for 48 h，apoptosis of Eca-109 cells was induced significantly，the expression of Bcl-2 protein was decreased and Bax protein was increased significantly than control group (P all <0.05). T he combination group showed more significant effect than either alone (P <0.05). CONCLUSION： Baohuoside-I in combination with 5-FU exerted a synergistic effect on inhibiting proliferation and promoting apoptosis. This effect associated with decreased expression of Bcl-2 and increased expression of Bax.

Key words: baohuoside-I, 5-FU, proliferation, apoptosis, Bcl-2, Bax, human esophageal carcinoma

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