乙醇诱导小鼠急性肝细胞损伤模型中NF-κB的表达

doi:10.3969/j.issn.1004-616x.2014.01.012

癌变·畸变·突变

乙醇诱导小鼠急性肝细胞损伤模型中NF-κB的表达

何宁，张静姝*，张静，姜淑卿，刘英华，管彤，李新

( 天津市疾病预防控制中心毒理室，天津 300011 )

收稿日期:2013-09-05 修回日期:2013-09-26 出版日期:2014-01-30 发布日期:2014-01-30
通讯作者: 张静姝，E-mail：rainbow7546@163.com
作者简介:何宁 (1977- )，女，河北唐山人，硕士，助理研究员，研究方向：毒理学。E-mail：hening777@163.com
基金资助:
天津市卫生局科技基金 (06KZ65)

Expression of nuclear factor-kappa B in mouse model of alcohol-induced acute hepatocyte injury

HE Ning，ZHANG Jing-shu*，ZHANG Jing，JIANG Shu-qing，LIU Ying-hua，GUAN Tong，LI Xin

(Tianjin Center for Disease Control and Prevention, Tianjin 300011, China)

Received:2013-09-05 Revised:2013-09-26 Online:2014-01-30 Published:2014-01-30
Contact: ZHANG Jing-shu，E-mail：rainbow7546@163.com

摘要/Abstract

摘要：

目的： 研究核因子NF-κB在乙醇诱导小鼠急性肝细胞损伤中的表达及意义。方法：40只小鼠随机分成对照组和3个乙醇 (250、500、1 000 mmol/kg)染毒组，每组10只。一次性灌胃染毒，16 h后检测小鼠血清中天冬氨酸转氨酶 (AST)、丙氨酸转氨酶 (ALT)的活力；染毒24 h后采用HE及油红O染色进行病理学观察；采用免疫组织化学法观察小鼠肝细胞中NF-κB蛋白的表达。结果：3个乙醇染毒组小鼠血清中的转氨酶AST和ALT活力均高于对照组 (P均<0.05)；HE染色发现小鼠肝组织中出现肝细胞内空泡变性。油红O染色显示损伤的肝细胞内出现大量脂滴。免疫组织化学检测发现500和1 000 mmol/kg乙醇染毒组小鼠肝细胞胞质及胞核内NF-κB蛋白表达的积分光密度明显高于对照组 (P<0.05)。结论：成功复制了急性小鼠酒精性肝损伤的模型，NF-κB升高是小鼠急性酒精性肝细胞损伤的一个重要分子事件。

关键词: NF-&kappa, B, 乙醇, 肝细胞, 损伤

Abstract:

OBJECTIVE: To investigate the expression and significance of nuclear factor-κappa B in alcohol-induced acute hepatocyte injury. METHODS：Forty mice were randomly divided into a control group and three alcohol treated groups (250，500，1 000 mmol/kg). Each group included ten mice. 16 h after gavage，serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured. After 24 h，the liver was examined by HE staining and oil Red O staining. The expression of NF-κB protein in hepatocytes was assessed by immunohistochemistry. RESULTS：Serum AST and ALT levels in three alcohol-treated groups were significantly increased compared with that in the control group (P<0.05). There was vacuolar degeneration in hepatocytes by HE staining. There were large amounts of Oil Red O staining droplets in the hepatocytes. In alcohol-treated groups (500， 1 000 mmol/kg)，the integrated optical density expression of NF-κB protein in hepatocytes cytoplasm and nucleus were significantly higher (P<0.05). CONCLUSION： This study successfully set up an alcohol-induced acute hepatocyte injury mouse model，and illustrated the role of NF-κB in the development and progression of acute alcoholic-induced hepatocyte injury.

Key words: NF-κB, alcohol, hepatocytes, injury

何宁，张静姝，张静，姜淑卿，刘英华，管彤，李新. 乙醇诱导小鼠急性肝细胞损伤模型中NF-κB的表达[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2014.01.012.

HE Ning，ZHANG Jing-shu，ZHANG Jing，JIANG Shu-qing，LIU Ying-hua，GUAN Tong，LI Xin. Expression of nuclear factor-kappa B in mouse model of alcohol-induced acute hepatocyte injury[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.01.012.

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乙醇诱导小鼠急性肝细胞损伤模型中NF-κB的表达

Expression of nuclear factor-kappa B in mouse model of alcohol-induced acute hepatocyte injury

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