健康成人自发染色体畸变率与微核率的本底状况再分析

doi:10.3969/j.issn.1004-616x.2014.01.013

癌变·畸变·突变

健康成人自发染色体畸变率与微核率的本底状况再分析

张学清，陈英*，王治东，王远，周平坤

( 军事医学科学院放射与辐射医学研究所，北京 100850 )

收稿日期:2013-06-21 修回日期:2013-10-16 出版日期:2014-01-30 发布日期:2014-01-30
通讯作者: 陈英，E-mail：yingchen_29@163.com
作者简介:张学清 (1976- )，女，浙江诸暨人，实验师，研究方向：细胞遗传学与辐射生物剂量学。
基金资助:
国家863课题资助项目 (2012AA063501)

Re-investigation on spontaneous frequencies of chromosome aberration and micronucleus in healthy adults

ZHANG Xue-qing，CHEN Ying*，WANG Zhi-dong，WANG Yuan，ZHOU Ping-kun

(Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China)

Received:2013-06-21 Revised:2013-10-16 Online:2014-01-30 Published:2014-01-30
Contact: CHEN Ying，E-mail：yingchen_29@163.com

摘要/Abstract

摘要：

目的： 了解环境因素对人类遗传物质的影响，以便正确评估放射从业人员以及低剂量辐射的健康效应。方法：选自北京市87名接受健康体检的成年人，每人用肝素钠抗凝管抽取外周静脉血2 mL。采用提前加秋水仙素和松胞素B法分别制备染色体与微核标本，Metafer自动分析系统结合人工分析染色体畸变与双核淋巴细胞微核，按不同年龄段与不同性别分组进行统计比较。结果：87名受检者的染色单体型畸变率平均为0.70%，染色体型畸变率为0.20%，其中双着丝粒体+环为0.13%，单纯无着丝粒体为0.06%。微核与微核细胞率分别为25.30‰和21.56‰。除单体型畸变外，染色体型畸变率与微核率均随年龄的增加而增加 (P<0.05)，不同性别之间差异无统计学意义 (P>0.05)。结论：健康成人染色体型畸变 (双着丝粒体+环)率与双核淋巴细胞微核率未超过国内外报道的本底范围，但较国内以往报道的本底值有增高趋势。

关键词: 成年人, 染色体畸变, 松胞素B微核, 自发率, 本底值

Abstract:

OBJECTIVE: In order to understand genetic effect from environment factors and to assess correctly the health effect of low dose radiation on occupational personnel. METHODS：The investigated objects came from 87 healthy adults who took routine physical examination and living in Beijing city. 2 mL peripheral venous blood from each person was drawn into heparinized tubes. Conventional chromosome culture with adding colchicines at the beginning and Cyt-B micronuclei (MN) methods were applied. Chromosomal aberrations (CA) and binucleated lymphocyte MN were analyzed using Metafer scanning system combined with manual work. Comparisons according to different age groups and both genders were carried out. RESULTS：The average spontaneous frequencies of chromatid-type and chromosomal-type aberrations in 87 tested persons were 0.70% and 0.20%，respectively. In chromosomal-type aberrations， the dicentrics plus rings (dic+r) were 0.13%，acentrics alone were 0.06%. Spontaneous MN and MNC frequencies were 23.43‰ and 20.47‰，respectively. Except chromatid-type aberration，spontaneous frequencies of CA and MN increased with age (P<0.05) and there were no distinct differences between genders (P>0.05). CONCLUSION： The spontaneous frequencies of chromosomal aberration (dic+r) and micronucleus did not exceed international background range，but showed a rising trend when compared with former reports in China.

Key words: adults, chromosome aberration, Cyt-B micronucleus, spontaneous frequency, background value

张学清，陈英，王治东，王远，周平坤. 健康成人自发染色体畸变率与微核率的本底状况再分析[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2014.01.013.

ZHANG Xue-qing，CHEN Ying，WANG Zhi-dong，WANG Yuan，ZHOU Ping-kun. Re-investigation on spontaneous frequencies of chromosome aberration and micronucleus in healthy adults[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.01.013.

参考文献

[1] Lloyd DC，Purrott RJ，Reeder EJ. The incidence of unstable chromosome aberrations in peripheral blood lymphocytes from unirradiated and occupationally exposed people[J]. Mutat Res，1980，72 (3)：523-532.

[2]白玉书，关树荣，张秀霞. 人淋巴细胞染色体畸变自发率及其与性别年龄关系的研究[J]. 癌变·畸变·突变，1994，6 (1)：37-40.

[3]白玉书，王知权，唐卫生，等. 对影响胞质分裂阻断微核自发率因素的探讨[J]. 中国辐射卫生，1992，1 (2)：81-82.

[4] International Atomic Energy Agency. Technical report series No.405. Cytogenetic analysis for radiation dose assessment[S]. Veinna：IAEA，2001：82-83.

[5]王继先，金璀珍，白玉书，等. 放射生物剂量学[M]. 北京：原子能出版社，1997：26.

[6]曹淑媛，周振英，李云华，等. 93例正常人的染色体分析[J]. 核防护，1978，3 (1)：1-6.

[7] Fenech M，Morley AA. cytokinesis-block micronucleus method in human lymphocytes：Effect of in vivo ageing and low-dose X-irradiation[J]. Mutat Res，1986，161 (2)：193-198.

[8] Fenech M，Neville S，Rinaldi J. Sex is an important variable affecting spontaneous micronucleus frequency in cytokinesis blocked lymphocytes[J]. Mutat Res，1994，313 (2/3)：203-207.

[9] Joksic G，Petrovic S，Ilic Z. Age-related changes in radiation-induced micronuclei among healthy adults[J]. Braz J Med & Biol Res，2004，37 (8)：1111-1117.

[10] Jones KH，York TP，Juusola J，et al. Genetic and environmental influences on spontaneous micronuclei frequencies in children and adults：a twin study[J]. Mutagenesis， 2011，26 (6)：745-752.

[11] 白玉书，陈德清. 人类辐射细胞遗传学[M]. 北京：人民卫生出版社，2006：111-112.

[12] International Atomic Energy Agency. Cytogenetic dosimetry： Applications in preparedness for and response to radiation emergencies[S]. Vienna：IAEA，2011：117.

[1]	杜秀明, 洪丽玲, 徐灵芝, 周庆云. 采用体外染色体畸变试验检测锐钛型纳米二氧化钛的遗传毒性[J]. 癌变·畸变·突变, 2022, 34(1): 67-71.
[2]	赵骅, 陆雪, 蔡恬静, 田梅, 刘青杰. 年龄和性别对正常人外周血淋巴细胞核质桥自发率的影响[J]. 癌变·畸变·突变, 2021, 33(1): 48-52.
[3]	原野, 陈丹丹, 荆淑芳, 于永生, 陈健恒, 吴纯启, 施畅. 正丁基硫代磷酰三胺对体细胞和生殖细胞的致突变性研究[J]. 癌变·畸变·突变, 2016, 28(6): 432-437.
[4]	张忠新, 张睿凤, 张慧芳, 段志凯. 常用辐射生物剂量计的研究现状[J]. 癌变·畸变·突变, 2016, 28(4): 325-328.
[5]	高梅, 曹冲, 英永, 马会, 贾庆文. 不同诱导方法制备大鼠肝微粒体酶及其活性的比较[J]. 癌变·畸变·突变, 2016, 28(4): 269-272.
[6]	应剑, 郝彬秀, 孟庆佳, 陈然, 王黎明, 王春玲. 茶树槲寄生“螃蟹脚”水提物的急性毒性和遗传毒性[J]. 癌变·畸变·突变, 2016, 28(4): 306-309,324.
[7]	罗雪婷, 蔡绮君, 赵博, 陈伟民, 陈晓, 张晓文. 紫荷植物固体饮料的急性毒性和遗传毒性试验研究[J]. 癌变·畸变·突变, 2016, 28(4): 302-305.
[8]	李爽，陆雪，赵骅，封江彬，刘青杰. 辐射诱导染色体畸变的快速FISH方法的建立[J]. 癌变·畸变·突变, 2014, 26(2): 123-126.
[9]	薄存香，刘永霞，戈扬，张振玲，谢琳. 2，4-二氯苯氧乙酸的致突变性研究[J]. 癌变·畸变·突变, 2014, 26(1): 68-70.
[10]	刘强,李克秋,杜利清,王彦,徐畅,樊赛军,李光,Markus Hecker. 电子垃圾污染物对人类遗传毒性的初步研究[J]. 癌变·畸变·突变, 2013, 25(6): 448-453.
[11]	陈颖,李莹,马爱翠,许丽,潘琦,刘向云,周莉,孙祖越. 染色体畸变试验中不同有机溶剂对CHL细胞毒性的比较[J]. 癌变·畸变·突变, 2013, 25(5): 373-376.
[12]	段长恩,公兵,杨秀滨. 聚丁二酸丁二醇酯浸提液对人外周血单核细胞的体外遗传毒性[J]. 癌变·畸变·突变, 2013, 25(3): 215-217.
[13]	董小艳. 四物汤抗环磷酰胺诱变作用的研究[J]. 癌变·畸变·突变, 2013, 25(1): 57-60.
[14]	陈逸青;刘丛云;陈勤. 沙苑子提取物对丝裂霉素C致小鼠骨髓细胞遗传损伤的保护作用[J]. , 2012, 24(4): 279-282.
[15]	高梅;曹冲;朱春花;曲保恩. 人参皂苷Rb2致中国仓鼠肺细胞染色体畸变的作用[J]. , 2012, 24(4): 298-301.

健康成人自发染色体畸变率与微核率的本底状况再分析

Re-investigation on spontaneous frequencies of chromosome aberration and micronucleus in healthy adults

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价