新疆维吾尔族女性乳腺癌与HLA-DRB1基因多态性的关系及临床意义

doi:10.3969/j.issn.1004-616x.2015.05.008

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (5): 366-369.doi: 10.3969/j.issn.1004-616x.2015.05.008

新疆维吾尔族女性乳腺癌与HLA-DRB1基因多态性的关系及临床意义

木妮热·木沙江, 古丽扎热叶·艾库拉, 贺春钰, 木克代斯·拜克提亚尔

新疆医科大学附属肿瘤医院乳腺放疗科, 新疆乌鲁木齐 830011

收稿日期:2015-04-24 修回日期:2015-07-25 出版日期:2015-09-30 发布日期:2015-09-30
作者简介:木妮热·木沙江,E-mail:muni66@aliyun.com
基金资助:
新疆维吾尔自治区自然科学基金(2013211A070)

Association and clinical significance of HLA-DRB1 gene polymorphisms with breast carcinogenesis in Uyghur women

MUSA·Munire, AIKULA·Gulzaria, HE Chunyu, BAHTIYAR ·Mukaddas

Department of Breast Radiotherapy, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang, China

Received:2015-04-24 Revised:2015-07-25 Online:2015-09-30 Published:2015-09-30

摘要/Abstract

摘要：

目的:研究新疆维吾尔族女性乳腺癌的发生与人类白细胞抗原(HLA) DRB1等位基因多态性的关系,探讨乳腺癌的遗传易感性。方法:分别收集维吾尔族女性乳腺癌患者和健康人外周血标本196和230例,提取细胞基因组DNA,采用序列特异性引物聚合酶链反应(PCR- SSP)和毛细管电泳测序(CE)的方法进行HLA-DRB1基因多态性鉴定。结果：乳腺癌患者外周血DNA的HLA-DRB1*01多态性频率显著高于健康对照(χ²=10.180,OR=4.550,P<0.05),HLA-DRB1*16多态性低于对照(χ²=4.792,OR=0.492,P<0.05),而其他位点多态性两组间的差异无统计学意义(P>0.05)。结论:维吾尔族女性乳腺癌的发生可能与HLA-DRB1等位基因多态性存在密切关系,对于揭示乳腺癌发病机制及临床诊断提供了客观依据。

关键词: 乳腺癌, 维吾尔族女性, 人类白细胞抗原-DRB1, 基因多态性

Abstract:

OBJECTIVE: To investigate the association of human leukocyte antigen(HLA) DRB1 gene polymorphisms with genetic susceptibility to breast cancer in Uyghur women. METHODS:Genomic DNA was isolated from peripheral blood of 196 patients with breast cancer and 230 healthy Uyghur women, HLA-DRB1 typing was performed by means of polymerase chain reaction-sequence-specific priming (PCR-SSP) and capillary electrophoresis sequencing (CE) method. RESULTS：The frequency of HLA-DRB1*01 genotype in breast cancer was significantly higher than in the control group (χ²=10.180, OR=4.550, P<0.05), while the frequency of HLA-DRB1*16 genotype was significantly lower than in the control group (χ²=4.792, OR=0.492, P<0.05). The frequencies of other HLA-DRB1 alleles did not show statistical significance between the two groups. CONCLUSION:Breast carcinogenesis in Uyghur women was closely associated with the allele polymorphisms of HLA-DRB1, which provides objective evidence for elucidating the molecular mechanism and clinical diagnosis for breast cancer.

Key words: breast cancer, Uyghur women, human leukocyte antigen-DRB1, gene polymorphisms

中图分类号:

R737.9

木妮热·木沙江, 古丽扎热叶·艾库拉, 贺春钰, 木克代斯·拜克提亚尔. 新疆维吾尔族女性乳腺癌与HLA-DRB1基因多态性的关系及临床意义[J]. 癌变·畸变·突变, 2015, 27(5): 366-369.

MUSA·Munire, AIKULA·Gulzaria, HE Chunyu, BAHTIYAR ·Mukaddas. Association and clinical significance of HLA-DRB1 gene polymorphisms with breast carcinogenesis in Uyghur women[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 366-369.

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新疆维吾尔族女性乳腺癌与HLA-DRB1基因多态性的关系及临床意义

Association and clinical significance of HLA-DRB1 gene polymorphisms with breast carcinogenesis in Uyghur women

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