POLH mRNA作为辐射损伤分子生物标志物的可行性研究

doi:10.3969/j.issn.1004-616x.2015.06.003

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (6): 421-425,431.doi: 10.3969/j.issn.1004-616x.2015.06.003

POLH mRNA作为辐射损伤分子生物标志物的可行性研究

李琳¹, 李孝鑫², 林仲武³, 扈江伟⁴, 张学清², 沈丽萍², 常诚², 陈英², 葛银林¹, 王治东²

1. 青岛大学医学院生物化学与分子生物学教研室, 山东青岛 266021;
2. 军事医学科学院放射与辐射医学研究所, 北京 100850;
3. 军事医学科学院科技部, 北京 100850;
4. 军事医学科学院附属307医院, 北京 100071

收稿日期:2015-06-16 修回日期:2015-10-27 出版日期:2015-11-30 发布日期:2015-11-30
通讯作者: 葛银林,E-mail:geyinlin@126.com;王治东,E-mail:wangzhidong1977@126.com E-mail:geyinlin@126.com;wangzhidong1977@126.com
作者简介:李琳,E-mail:longyun0515@163.com。
基金资助:
全军医学科技"十二五"科研项目(BWS11J018);全军后勤科研重大项目(AWS14C014);解放军307医院院创新基金(JS-2014-04)

Feasibility study on expression of POLH mRNA as a molecular biomarker for radiation injury

LI Lin¹, LI Xiaoxin², LIN Zhongwu³, HU Jiangwei⁴, ZHANG Xueqing², SHEN Liping², CHANG Cheng², CHEN Ying², GE Yinlin¹, WANG Zhidong²

1. Department of Biochemistry and Molecular Biology, College of Medical, Qingdao University, Qingdao 266021, Shandong;
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850;
3. Ministry of Science and Technology, Academy of Military Medical Sciences, Beijing 100850;
4. 307 Affiliated Hospital of Military Medical Sciences, Beijing 100071, China

Received:2015-06-16 Revised:2015-10-27 Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 目的:研究受γ射线照射后人外周血中POLH mRNA的表达变化,为确立POLH mRNA作为新的辐射损伤分子生物标志物提供实验依据。方法:采取3名健康成人的外周血,利用⁶⁰Co γ放射源分别对其进行0.5、1、2、4、6、10 Gy照射,在照射后2、4、8、12和24 h收集各个剂量组受试者全血,提取总RNA,利用TaqMan探针实时定量PCR法,检测POLH mRNA的相对表达量。另收集30名不同年龄段(男女各占一半)的健康人外周血作为本底组,检测POLH mRNA在不同个体间的表达差异。结果:照射后2 h,仅10 Gy照射组POLH mRNA表达增加,其余各照射组无显著变化;照射后4、8、12和24 h,在0~2 Gy范围内,POLH mRNA表达量随照射剂量的增加而增加,呈现明显的剂量依赖效应,大于2 Gy照射后增加趋势接近饱和。照射后4~12 h随时间的增加表达量也不断增加,于12 h达到峰值。在对健康人群本底表达水平的分析中发现,POLH mRNA表达水平不受性别、年龄影响,在不同个体间相对均一,波动范围小。结论:POLH mRNA可由为辐射诱导表达,在一定时间和剂量范围内呈现明显的剂量依赖关系,且在不同个体之间本底表达水平相对均一,具备作为辐射损伤分子生物标志物的潜力。

关键词: 辐射损伤, 分子生物标志物, POLH, 实时定量PCR

Abstract: OBJECTIVE: To explore the change in expression of POLH mRNA in human peripheral blood exposed to γ-ray and provide experimental evidence for POLH mRNA to be a new molecular biomarker for radiation injury. METHODS: After radiation with dose of 0,0.5,1,2,4,6,10 Gy by ⁶⁰Co γ-rays,total RNA was extracted from peripheral blood of three healthy adults at 0,2,4,8,12,24 h post-radiation. Expression level of POLH mRNA was measured by real-time quantitative PCR. 30 healthy donors' peripheral blood were collected as the background group to measure the expression level of POLH mRNA in different individuals. RESULTS: The expression level of POLH mRNA increased in a dose-dependent manner within the range of 0-2 Gy at 4,8,12,and 24 h after radiation,with no significant change at 2 h post-radiation. Over 2 Gy,the expression level of POLH mRNA was close to saturation. It also showed that the expression level of POLH mRNA increased in a time-dependent manner within the range of 4-12 h postradiation,a maximum response was observed at 12 h. The basal level of POLH mRNA was not affected by age and gender. CONCLUSION: POLH mRNA was induced by radiation and increased in dose-dependent and time-dependent manners. The back ground level in healthy human peripheral blood was uniform. Our results suggested that POLH mRNA may be a useful radiation biomarker.

Key words: radiation injury, molecular biomarker, POLH, real-time quantitative PCR

中图分类号:

Q345

李琳, 李孝鑫, 林仲武, 扈江伟, 张学清, 沈丽萍, 常诚, 陈英, 葛银林, 王治东. POLH mRNA作为辐射损伤分子生物标志物的可行性研究[J]. 癌变·畸变·突变, 2015, 27(6): 421-425,431.

LI Lin, LI Xiaoxin, LIN Zhongwu, HU Jiangwei, ZHANG Xueqing, SHEN Liping, CHANG Cheng, CHEN Ying, GE Yinlin, WANG Zhidong. Feasibility study on expression of POLH mRNA as a molecular biomarker for radiation injury[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 421-425,431.

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POLH mRNA作为辐射损伤分子生物标志物的可行性研究

Feasibility study on expression of POLH mRNA as a molecular biomarker for radiation injury

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