纳豆脂肽诱导人乳腺癌细胞MCF-7凋亡的机制研究

doi:10.3969/j.issn.1004-616x.2015.06.011

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (6): 459-462.doi: 10.3969/j.issn.1004-616x.2015.06.011

纳豆脂肽诱导人乳腺癌细胞MCF-7凋亡的机制研究

刘瑞雪, 安秀峰, 冯莉, 李勇超, 张波

北京联合大学应用文理学院, 生物活性物质与功能食品北京市重点实验室, 北京 100191

收稿日期:2015-07-21 修回日期:2015-10-14 出版日期:2015-11-30 发布日期:2015-11-30
通讯作者: 张波,E-mail:zhangbo_wl@buu.edu.cn E-mail:zhangbo_wl@buu.edu.cn
作者简介:刘瑞雪,E-mail:liuruixue871024@163.com;Tel:15311939337。
基金资助:
"十二五"国家科技支撑计划课题

Mechanisms of natto lipopeptide-induced apoptosis in MCF-7 cells

LIU Ruixue, AN Xiufeng, FENG Li, LI Yongchao, ZHANG Bo

Key Laboratory for Bioactive Substances and Functional Food, College of Arts and Science, Beijing Union University, Beijing 100191, China

Received:2015-07-21 Revised:2015-10-14 Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 目的:初步探讨纳豆脂肽诱导人乳腺癌细胞MCF-7凋亡的可能作用机制。方法:取对数生长期的MCF-7细胞,实验设4组,分别加入不同浓度(0、1、2、4μg/mL)的纳豆脂肽,于37℃培养箱中继续培养12 h后,收集细胞,采用流式细胞术检测纳豆脂肽对MCF-7细胞凋亡的影响;单细胞凝胶电泳检测纳豆脂肽对MCF-7细胞DNA损伤的影响;Western blot法检测纳豆脂肽对雌激素受体ERα和ERβ蛋白表达的影响。结果:与对照组比较,各浓度纳豆脂肽处理组均可诱导MCF-7细胞凋亡(P均<0.05);Olive尾矩和尾部DNA含量均明显增加(P<0.05或P<0.01),且4μg/mL纳豆脂肽组尾长亦明显增加(P<0.01)。纳豆脂肽浓度为4μg/mL时,ERα表达水平明显下降,ERβ表达水平明显上升,差异均具有统计学意义(P均<0.05)。结论:纳豆脂肽可能是通过影响MCF-7细胞内雌激素受体表达水平和引起DNA损伤来诱导MCF-7细胞发生凋亡。

关键词: 纳豆脂肽, MCF-7细胞, 细胞凋亡, DNA损伤, 雌激素受体

Abstract: OBJECTIVE: To investigate the mechanisms of apoptosis induced by natto lipopeptide in MCF-7 cells. METHODS: Logarithmic growth phase of MCF-7 cells were treated with natto lipopeptide at different concentration(0,1,2,4μg/mL) in vitro and cultuerd at 37℃ incubator. Cells were collected after 12 h. Flow cytometry was used to detect apoptosis;single cell gel electrophoresis was used to detect DNA damage and Western blot for detection of estrogen receptors α and β expression levels. RESULTS: Natto lipopeptide could induce apoptosis in MCF-7 cells(P<0.05). Olive tail moment and tail DNA levels increased significantly(P<0.05 or P<0.01),and 4μg/mL natto lipopeptide group significantly increased tail length compared wie control group(P<0.01). Compared wie control group,natto lipopeptide of 4μg/mL also had significant effects on the expressions of ERα and ERβ(P<0.05). CONCLUSION: Natto lipopeptide exposure could change the expression levels of ERα and ERβ and damage DNA of MCF-7 cells,contributing to apoptosis.

Key words: natto lipopeptide, MCF-7 cells, apoptosis, DNA damage, estrogen receptors

中图分类号:

R730.5

刘瑞雪, 安秀峰, 冯莉, 李勇超, 张波. 纳豆脂肽诱导人乳腺癌细胞MCF-7凋亡的机制研究[J]. 癌变·畸变·突变, 2015, 27(6): 459-462.

LIU Ruixue, AN Xiufeng, FENG Li, LI Yongchao, ZHANG Bo. Mechanisms of natto lipopeptide-induced apoptosis in MCF-7 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 459-462.

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纳豆脂肽诱导人乳腺癌细胞MCF-7凋亡的机制研究

Mechanisms of natto lipopeptide-induced apoptosis in MCF-7 cells

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