关键词: 食管鳞癌, KIAA1522, 转录本, 表观分子量

Abstract: OBJECTIVE:To detect the expression and abundance of different transcripts of KIAA1522 gene in esophageal squamous cell carcinoma (ESCC) tissues and cell lines,and to determine the apparent molecular weight of the protein. METHODS:The expression and abundance of four different transcripts of KIAA1522 gene in ESCC tissues and cell lines were detected by reverse transcription PCR(RT-PCR) and quantitative real-time PCR(qPCR). Molecular weight of the KIAA1522 protein was determined by Western blot together with experiments that involved KIAA1522 gene specific knockdown or exogenous over-expression. The sequence of KIAA1522 protein was confirmed by mass spectrometry. RESULTS:Both T1 and T4 transcripts of KIAA1522 were expressed in ESCC tissues and cell lines,but T1 was the most abundant. The 170 kDa band observed in the Western blot was the KIAA1522 gene-specific protein expression product. CONCLUSION:T1 was the predominant transcript of KIAA1522 gene in ESCC tissues and cell lines. The apparent molecular weight of KIAA1522 protein was 170 kDa.

Key words: esophageal squamous cell carcinoma, KIAA1522, transcripts, apparent molecular weight