癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (4): 309-312.doi: 10.3969/j.issn.1004-616x.2017.04.013

• 检测研究 • 上一篇    下一篇

采用Ames试验检测5种塑胶跑道面层浸提液的致突变性

江漪, 贾肖辉, 李培宁, 陈秀娟, 刘冬虹, 刘香梅   

  1. 广州质量监督检测研究院, 广东 广州 511447
  • 收稿日期:2017-02-27 修回日期:2017-05-08 出版日期:2017-07-31 发布日期:2017-07-31
  • 通讯作者: 刘香梅,E-mail:283830090@qq.com E-mail:283830090@qq.com
  • 作者简介:江漪,E-mail:976506475@qq.com。

Ames mutagenicity assay on five plastic surface chemicals in racetrack

JIANG Yi, JIA Xiaohui, LI Peining, CHEN Xiujuan, LIU Donghong, LIU Xiangmei   

  1. Guangzhou Quality Supervision and Testing Institute, Guangzhou 511447, Guangdong, China
  • Received:2017-02-27 Revised:2017-05-08 Online:2017-07-31 Published:2017-07-31

摘要:

目的:探讨5种不同合成材料的塑胶跑道面层浸提液的致突变性。方法:分别采用纯水和二甲基亚砜(DMSO)两种浸提介质按0.2 g/mL浸提处理硅PU球场、透气型塑胶跑道、EPDM塑胶跑道、混合型塑胶跑道、预制型卷材跑道5种塑胶跑道面层,在加与不加体外活化代谢系统的情况下进行Ames试验。结果:透气型塑胶跑道面层的DMSO浸提液,在体外代谢活化系统条件下,TA97a、TA100回变菌落数大于溶剂对照组的2倍。EPDM塑胶跑道面层的DMSO浸提液,无论在加或不加体外代谢活化系统条件下,TA97a、TA100回变菌落数均大于溶剂对照组的2倍。5种塑胶跑道面层的纯水浸提液均未引起回变菌落数异常。结论:透气型塑胶跑道面层、EPDM塑胶跑道面层的DMSO浸提液具有致突变性。

关键词: 塑胶跑道面层, 二甲基亚砜, 致突变性, Ames试验

Abstract:

OBJECTIVE: To explore the mutagenicity of five different synthetic sport surface chemicals. METHODS: Plastic racetracks from:silicon PU stadium,permeable running tracks,EPDM running tracks,mixed running tracks and prefabricated roll,were extracted using water and dimethyl sulfoxide (DMSO),and were tested in the Ames assays. RESULTS: Using tester strains TA97a and TA100 with S9,the revertant colonies of DMSO-extracted products from permeable running tracks surface were two times higher than that in the controls. Using the same tester strains but with or without S9, the revertant colonies of DMSO-extracted EPDM running tracks surface were also two times higher than that in the controls. On the other hand,water-extracted products did not induce mutagenicity. CONCLUSION: DMSO-extracted products from both permeable and EPDM running tracks surfaces were capable of inducing mutagenicity.

Key words: plastic running tracks surface, dimethyl sulfoxide, mutagenicity, Ames test

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