协同刺激分子B7-H4在小鼠食管癌前病变中的表达

doi:10.3969/j.issn.1004-616x.2018.04.004

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (4): 263-269,274.doi: 10.3969/j.issn.1004-616x.2018.04.004

协同刺激分子B7-H4在小鼠食管癌前病变中的表达

陈欣然¹, 单保恩²

1. 河北医科大学第四医院药学部, 河北石家庄 050011;
2. 河北医科大学第四医院科研中心, 河北石家庄 050011

收稿日期:2017-11-15 修回日期:2018-05-10 出版日期:2018-07-30 发布日期:2018-07-30
通讯作者: 单保恩,E-mail:hbthshan@163.com E-mail:hbthshan@163.com
作者简介:陈欣然,E-mail:cxrtht@163.com
基金资助:
河北省科技计划项目（162777174）

Expression of costimulatory molecule B7-H4 during progression of esophageal precancerous conditions in mice

CHEN Xinran¹, SHAN Baoen²

1. Department of Pharmacy, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011;
2. Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China

Received:2017-11-15 Revised:2018-05-10 Online:2018-07-30 Published:2018-07-30

摘要/Abstract

摘要： 目的：研究协同刺激分子B7同系物4（B7-H4）在小鼠食管癌前病变中的表达变化，探讨其在食管鳞状细胞癌发生中的作用。方法：133只C57BL/6小鼠，分为对照组42只和诱癌组91只。诱癌组小鼠采用饮水法给予50 μg/mL 4-硝基喹啉-1-氧化物（4NQO）15周，诱导小鼠食管癌前病变。通过苏木素-伊红（HE）染色法评价食管组织病理变化；免疫组化和Western blot检测B7-H4蛋白在食管组织中的表达，Western blot检测B7-H4蛋白在小鼠血清中的表达。结果：从16~28周，4NQO诱癌组小鼠食管组织出现肉眼可见的增厚、凸起、肿块等病理改变。HE染色结果显示，小鼠食管组织癌前病变级别与诱癌时间呈正相关（r=0.55，P < 0.01）。另外，B7-H4在食管组织的表达水平与食管癌前病变级别呈正相关（r=0.57，P < 0.01）。与对照组相比，第26周和第28周诱癌组小鼠食管组织B7-H4蛋白表达显著升高（P < 0.05或P < 0.01）。与正常小鼠相比，高级别上皮内瘤变小鼠血清B7-H4蛋白浓度显著升高（P < 0.05）。结论：小鼠食管癌前病变进程中，B7-H4蛋白在血清和食管组织中表达上调，B7-H4可能参与了食管癌前病变的进程。

关键词: 4-硝基喹啉-1-氧化物, 协同刺激分子, B7-H4, 食管癌前病变

Abstract: OBJECTIVE: To investigate the costimulatory molecule B7 homolog 4 (B7-H4) expression during progression of esophageal precancerous lesions in mice and to evaluate its clinical significance in esophageal squamous cell carcinoma formation. METHODS: 133 C57BL/6 mice were assigned to the control group (n=42) or 4NQO model group (n=91). 50 μg/mL 4-Nitroquinoline -1-oxide (4NQO) in drinking water was fed to the 4NQO model mice for 15 weeks. Hematoxylin eosin (HE) staining was used to evaluate changes in pathological stages of esophageal tissues. B7-H4 protein expression in esophageal tissues was detected by immunohistochemical staining and Western blot. B7-H4 protein expression in serum was detected by Western blot. RESULTS: From 16th to 28th week,esophageal showed visible pathological changes,such as incrassation,pimplings and lumps. Furthermore,HE staining showed that there was a significant and positive correlation between the esophageal pathological grade and the induction time of carcinogenesis,(r=0.55,P < 0.01). Additionally,B7-H4 protein expression in esophageal tissue was positively correlated with the pathological grade of precancerous lesions(r=0.57,P < 0.01). At 26 th and 28 th week, B7-H4 protein expression increased significantly compared with the control group(P < 0.05 or P < 0.01). Moreover, B7-H4 protein concentration in sera of mice of high grade intraepithelial neoplasia increased significantly compared with the normal mice,P < 0.05. CONCLUSION: B7-H4 expression in esophageal and sera increased gradually during the progression of esophageal precancerous lesions. Therefore,B7-H4 may be involved in the formation of esophageal precancerous conditions.

Key words: 4NQO, costimulatory molecule, B7-H4, esophageal precancerous condition

中图分类号:

R735.1

陈欣然, 单保恩. 协同刺激分子B7-H4在小鼠食管癌前病变中的表达[J]. 癌变·畸变·突变, 2018, 30(4): 263-269,274.

CHEN Xinran, SHAN Baoen. Expression of costimulatory molecule B7-H4 during progression of esophageal precancerous conditions in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 263-269,274.

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协同刺激分子B7-H4在小鼠食管癌前病变中的表达

Expression of costimulatory molecule B7-H4 during progression of esophageal precancerous conditions in mice

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[1]	陈欣然, 宋永志, 单保恩. 协同刺激分子B7-H4在食管癌前病变组织中的表达及临床意义[J]. 癌变·畸变·突变, 2018, 30(5): 368-373.
[2]	陈欣然, 王玲, 王薇, 韩利霞, 刘莉, 单保恩. 4-硝基喹啉-1-氧化物诱导食管鳞状细胞癌发生的研究进展[J]. 癌变·畸变·突变, 2016, 28(2): 151-154.
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