白藜芦醇对人结肠细胞NCM460和SW620增殖及基因组不稳定性的影响

doi:10.3969/j.issn.1004-616x.2019.04.004

癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (4): 283-288.doi: 10.3969/j.issn.1004-616x.2019.04.004

白藜芦醇对人结肠细胞NCM460和SW620增殖及基因组不稳定性的影响

曹宇¹, 张乔晔¹, 花蕊¹, 马晓玲², 汪旭^1,3, 倪娟^1,3

1. 云南师范大学生命科学学院, 云南昆明 650500;
2. 上海三誉华夏基因科技有限公司, 上海 201101;
3. 云南师范大学生物能源持续开发与利用教育部工程研究中心, 云南昆明 650500

收稿日期:2019-04-15 修回日期:2019-07-05 出版日期:2019-07-30 发布日期:2019-08-23
通讯作者: 倪娟,E-mail:gt_gg30@163.com E-mail:gt_gg30@163.com
作者简介:曹宇,E-mail:xswdxzl@yeah.net。
基金资助:
国家自然科学基金（31560307，31860301）

Effects of resveratrol on proliferation and genomic instability of human colon cells NCM460 and SW620

CAO Yu¹, ZHANG Qiaoye¹, HUA Rui¹, MA Xiaoling², WANG Xu^1,3, NI Juan^1,3

1. School of Life Science, Yunnan Normal University, Kunming 650500;
2. Shanghai Sanyu China Gene Science & Technology Co., Ltd., Shanghai 201101;
3. Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming 650500, Yunnan, China

Received:2019-04-15 Revised:2019-07-05 Online:2019-07-30 Published:2019-08-23

摘要/Abstract

摘要： 目的：探讨白藜芦醇（RSV）对人结肠上皮细胞株NCM460及结肠癌细胞株SW620增殖和基因组不稳定性（GIN）的影响。方法：选用NCM460和SW620细胞为研究材料，以台盼兰拒染法检测不同浓度RSV（3.125、6.25、12.5、25、50、100 μmol/L）对细胞增殖的影响；以CBMN-Cyt和集落形成实验分别检测不同浓度RSV（25、100 μmol/L）对GIN和细胞集落形成能力的影响。结果：与对照组相比，6.25~100 μmol/L的RSV对SW620细胞的增殖具有明显的抑制作用（P < 0.05），100 μmol/L的RSV处理后细胞凋亡率增加、GIN上升、细胞集落形成能力降低（P < 0.05或0.01）；同时，相同浓度的RSV可以降低NCM460细胞的GIN，促进细胞分裂并增强细胞集落形成能力（P均 < 0.05）。结论：RSV可维护正常细胞结肠上皮细胞NCM460的基因组稳定性，同时显著提高结肠癌细胞SW620的GIN发生率，可能是其在结肠癌治疗中表现出较好的抗癌效应及正常细胞保护作用的分子基础。

关键词: 白藜芦醇, 细胞增殖, 基因组不稳定性, 细胞凋亡, 集落形成实验

Abstract: OBJECTIVE:To investigate the effects of resveratrol (RSV) on the genome instability (GIN) and proliferation of human colonic epithelial cell line NCM460 and colon cancer cell line SW620. METHODS:Effects of different concentrations of RSV (3.125,6.25,12.5,25,50,100 μmol/L) on cell proliferation were detected using the trypan blue exclusion method. CBMN-Cyt and colony formation experiments were performed to examine the effects of different concentrations of RSV (25,100 μmol/L) on GIN and clonal formation ability. RESULTS:Compared with the control group,RSV of 6.25-100 μmol/L significantly inhibited the proliferation of SW620 cells (P < 0.05). RSV of 100 μmol/L significantly increased the apoptosis rates,increased the GIN of SW620 cells and decreased the ability of clonal formation (P < 0.05 or 0.01). On the other hand,the same concentration of RSV decreased the GIN of NCM460 cells,enhanced cell division and cell clonality (all P < 0.05). CONCLUSION:The data show that RSV was able to maintain the genomic stability of the normal NCM460 epithelial cells but significantly increased the incidence of GIN in the SW620 colon cancer cells which could be the molecular basis for its excellent anticancer effect and normal cell protection in colon cancer therapy.

Key words: resveratrol, cell proliferation, genomic instability, apoptosis, colony formation assay

中图分类号:

R73-3

曹宇, 张乔晔, 花蕊, 马晓玲, 汪旭, 倪娟. 白藜芦醇对人结肠细胞NCM460和SW620增殖及基因组不稳定性的影响[J]. 癌变·畸变·突变, 2019, 31(4): 283-288.

CAO Yu, ZHANG Qiaoye, HUA Rui, MA Xiaoling, WANG Xu, NI Juan. Effects of resveratrol on proliferation and genomic instability of human colon cells NCM460 and SW620[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 283-288.

参考文献

[1] BONASSI S, ZNAOR A, CEPPI M, et al. An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans[J]. Carcinogenesis, 2007, 28(3):625-631.
[2] ANDREASSI M G, BARALE R, IOZZO P, et al. The association of micronucleus frequency with obesity, diabetes and cardiovascular disease[J]. Mutagenesis, 2011, 26(1):77-83.
[3] MIGLIORE L, COPPEDè F, FENECH M, et al. Association of micronucleus frequency with neurodegenerative diseases[J]. Mutagenesis, 2011, 26(1):85-92.
[4] NI J, GUO X H, WANG H, et al. Differences in the effects of EGCG on chromosomal stability and cell growth between normal and colon cancer cells[J]. Molecules, 2018, 23(4):E788.
[5] GUO X H, WANG H, NI J, et al. Geraniin selectively promotes cytostasis and apoptosis in human colorectal cancer cells by inducing catastrophic chromosomal instability[J]. Mutagenesis, 2018, 33(4):271-281.
[6] ABDELGAWAD I Y, GRANT M K O, ZORDOKY B N. Leveraging the cardio-protective and anticancer properties of resveratrol in cardio-oncology[J]. Nutrients, 2019, 11(3):E627.
[7] JANG M, CAI L, UDEANI G O, et al. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes[J]. Science, 1997, 275(5297):218-220.
[8] WEISKIRCHEN S, WEISKIRCHEN R. Resveratrol:how much wine do You have to drink to stay healthy?[J]. Adv Nutr, 2016, 7(4):706-718.
[9] STANKIEWICZ P, LUPSKI J R. Structural variation in the human genome and its role in disease[J]. Annu Rev Med, 2010, 61:437-455.
[10] NI J, LIANG Z Q, ZHOU T, et al. A decreased micronucleus frequency in human lymphocytes after folate and vitamin B12 intervention:a preliminary study in a Yunnan population[J]. Int J Vitam Nutr Res, 2012, 82(6):374-382.
[11] SAMPER E, NICHOLLS D G, MELOV S. Mitochondrial oxidative stress causes chromosomal instability of mouse embryonic fibroblasts[J]. Aging Cell, 2003, 2(5):277-285.
[12] CIRCU M L, AW T Y. Reactive oxygen species, cellular redox systems, and apoptosis[J]. Free Radic Biol Med, 2010, 48(6):749-762.
[13] WILLCOX J K, ASH S L, CATIGNANI G L. Antioxidants and prevention of chronic disease[J]. Crit Rev Food Sci Nutr, 2004, 44(4):275-295.
[14] IRANI K, XIA Y, ZWEIER J L, et al. Mitogenic signaling mediated by oxidants in Ras-transformed fibroblasts[J]. Science, 1997, 275(5306):1649-1652.
[15] SABLINA A A, BUDANOV A V, ILYINSKAYA G V, et al. The antioxidant function of the p53 tumor suppressor[J]. Nat Med, 2005, 11(12):1306-1313.
[16] VAFA O, WADE M, KERN S, et al. C-Myc can induce DNA damage, increase reactive oxygen species, and mitigate p53 function:a mechanism for oncogene-induced genetic instability[J]. Mol Cell, 2002, 9(5):1031-1044.
[17] YOUSEF M, VLACHOGIANNIS I A, TSIANI E. Effects of resveratrol against lung cancer:in vitro and in vivo studies[J]. Nutrients, 2017, 9(11):E1231.
[18] MA S, FENG J, ZHANG R, et al. SIRT1 activation by resveratrol alleviates cardiac dysfunction via mitochondrial regulation in diabetic cardiomyopathy mice[J]. Oxid Med Cell Longev, 2017, 2017:4602715.
[19] FOURNY N, LAN C, SéRéE E, et al. Protective effect of resveratrol against ischemia-reperfusion injury via enhanced high energy compounds and eNOS-SIRT1 expression in type 2 diabetic female rat heart[J]. Nutrients, 2019, 11(1):E105.
[20] KITADA M, KUME S, IMAIZUMI N, et al. Resveratrol improves oxidative stress and protects against diabetic nephropathy through normalization of Mn-SOD dysfunction in AMPK/SIRT1-independent pathway[J]. Diabetes, 2011, 60(2):634-643.
[21] KIM E N, LIM J H, KIM M Y, et al. Resveratrol, an Nrf2 activator, ameliorates aging-related progressive renal injury[J]. Aging (Albany NY), 2018, 10(1):83-99.
[22] CARLOS-REYES á, LóPEZ-GONZáLEZ J S, MENESES-FLORES M, et al. Dietary compounds as epigenetic modulating agents in cancer[J]. Front Genet, 2019, 10:79.
[23] LINK A, BALAGUER F, GOEL A. Cancer chemoprevention by dietary polyphenols:promising role for epigenetics[J]. Biochem Pharmacol, 2010, 80(12):1771-1792.
[24] KAI L, SAMUEL S K, LEVENSON A S. Resveratrol enhances p53 acetylation and apoptosis in prostate cancer by inhibiting MTA1/NuRD complex[J]. Int J Cancer, 2010, 126(7):1538-1548.
[25] MEDINA-AGUILAR R, PéREZ-PLASENCIA C, MARCHAT L A, et al. Methylation landscape of human breast cancer cells in response to dietary compound resveratrol[J/OL]. PLoS One, 2016, 11(6):e0157866. doi:10.1371/journal.pone.0157866.

白藜芦醇对人结肠细胞NCM460和SW620增殖及基因组不稳定性的影响

Effects of resveratrol on proliferation and genomic instability of human colon cells NCM460 and SW620

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	赵岩, 孙震晓. 二甲双胍抑制人MDA-MB-231细胞增殖及迁移的体外实验研究[J]. 癌变·畸变·突变, 2022, 34(1): 35-39.
[2]	李柏茹, 秦双建, 关岚, 李闰冰, 蔡颖, 蒲桔宁, 曾明, 肖芳, 徐新云. PM_2.5对HK-2细胞氧化损伤和凋亡的影响[J]. 癌变·畸变·突变, 2021, 33(5): 360-364.
[3]	黄晓华, 牛永东, 石刚刚. 核受体FXR激活对宫颈癌CaSki细胞增殖的抑制效应及其作用机制[J]. 癌变·畸变·突变, 2021, 33(4): 302-306.
[4]	王熙凯, 孟庆贺, 高燕鲁. miR-223-3p通过调控ECT2诱导非小细胞肺癌细胞凋亡的实验研究[J]. 癌变·畸变·突变, 2021, 33(3): 187-192.
[5]	郑珺文, 常晨, 郝佳洁, 蔡岩, 王明荣, 张钰. NEDD4在食管鳞癌中的表达及作用研究[J]. 癌变·畸变·突变, 2021, 33(3): 203-207,224.
[6]	张娜, 范志露, 杨荔艳, 常晨, 蔡岩, 郝佳洁, 王明荣. 食管鳞癌中SERPINB5表达降低的作用机制及其临床意义[J]. 癌变·畸变·突变, 2021, 33(2): 101-109.
[7]	马顺鹏, 王全凯, 王苗, 宋佳阳, 乌瀚宝栎尔, 谢广云, 许建宁. 甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化过程中细胞凋亡相关lncRNA的筛选及功能研究[J]. 癌变·畸变·突变, 2021, 33(1): 1-5,31.
[8]	黄立利, 张梦云, 刘科亮, 庞定国, 刘丽达, 徐培渝. 2,4-二氯苯氧乙酸对幼龄SD大鼠肝毒性的研究[J]. 癌变·畸变·突变, 2021, 33(1): 22-27.
[9]	兰莉, 陈海丽, 徐蕾. RBMS3-AS3对宫颈癌细胞增殖、凋亡和侵袭的影响及作用机制[J]. 癌变·畸变·突变, 2020, 32(6): 438-443.
[10]	杨兴肖, 张雪原, 邹乃祎, 单保恩, 马鸣, 祝淑钗. HMGB1 siRNA干扰对食管鳞癌细胞放射敏感性的影响[J]. 癌变·畸变·突变, 2020, 32(4): 269-274.
[11]	华辰凤, 赵俊伟, 尚平平, 樊美娟, 谢复炜, 李翔. 不同焦油释放量卷烟全烟气气-液界面暴露致细胞毒性、炎症因子释放和细胞凋亡的研究[J]. 癌变·畸变·突变, 2020, 32(3): 182-186.
[12]	王呈谕, 刘晓璇, 李轶群, 李登科, 孙震晓. 何首乌、虎杖、大黄水提物中游离蒽醌含量的测定及对人正常肝细胞的毒性作用[J]. 癌变·畸变·突变, 2020, 32(3): 215-220.
[13]	方从文, 石莹, 杜凤, 康秉文, 韩明博, 殷草草, 柏桦, 秦绪军. 高迁移率族蛋白B1在小鼠肝再生中对细胞增殖的作用及机制[J]. 癌变·畸变·突变, 2020, 32(2): 81-86.
[14]	陈婉娴, 王思梦, 陈张瑜, 王潘集, 许丽艳, 李恩民. 细胞分裂周期蛋白42在肿瘤中的作用及其分子机制研究进展[J]. 癌变·畸变·突变, 2020, 32(2): 159-161.
[15]	万鹰昕, 张平, 李楠. 亚硒酸钠与白藜芦醇联用对肺癌细胞抗氧化和细胞周期蛋白的影响[J]. 癌变·畸变·突变, 2019, 31(6): 459-463.