癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (4): 283-288.doi: 10.3969/j.issn.1004-616x.2019.04.004

• 论著 • 上一篇    下一篇

白藜芦醇对人结肠细胞NCM460和SW620增殖及基因组不稳定性的影响

曹宇1, 张乔晔1, 花蕊1, 马晓玲2, 汪旭1,3, 倪娟1,3   

  1. 1. 云南师范大学生命科学学院, 云南 昆明 650500;
    2. 上海三誉华夏基因科技有限公司, 上海 201101;
    3. 云南师范大学生物能源持续开发与利用教育部工程研究中心, 云南 昆明 650500
  • 收稿日期:2019-04-15 修回日期:2019-07-05 出版日期:2019-07-30 发布日期:2019-08-23
  • 通讯作者: 倪娟,E-mail:gt_gg30@163.com E-mail:gt_gg30@163.com
  • 作者简介:曹宇,E-mail:xswdxzl@yeah.net。
  • 基金资助:
    国家自然科学基金(31560307,31860301)

Effects of resveratrol on proliferation and genomic instability of human colon cells NCM460 and SW620

CAO Yu1, ZHANG Qiaoye1, HUA Rui1, MA Xiaoling2, WANG Xu1,3, NI Juan1,3   

  1. 1. School of Life Science, Yunnan Normal University, Kunming 650500;
    2. Shanghai Sanyu China Gene Science & Technology Co., Ltd., Shanghai 201101;
    3. Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming 650500, Yunnan, China
  • Received:2019-04-15 Revised:2019-07-05 Online:2019-07-30 Published:2019-08-23

摘要: 目的:探讨白藜芦醇(RSV)对人结肠上皮细胞株NCM460及结肠癌细胞株SW620增殖和基因组不稳定性(GIN)的影响。方法:选用NCM460和SW620细胞为研究材料,以台盼兰拒染法检测不同浓度RSV(3.125、6.25、12.5、25、50、100 μmol/L)对细胞增殖的影响;以CBMN-Cyt和集落形成实验分别检测不同浓度RSV(25、100 μmol/L)对GIN和细胞集落形成能力的影响。结果:与对照组相比,6.25~100 μmol/L的RSV对SW620细胞的增殖具有明显的抑制作用(P < 0.05),100 μmol/L的RSV处理后细胞凋亡率增加、GIN上升、细胞集落形成能力降低(P < 0.05或0.01);同时,相同浓度的RSV可以降低NCM460细胞的GIN,促进细胞分裂并增强细胞集落形成能力(P均 < 0.05)。结论:RSV可维护正常细胞结肠上皮细胞NCM460的基因组稳定性,同时显著提高结肠癌细胞SW620的GIN发生率,可能是其在结肠癌治疗中表现出较好的抗癌效应及正常细胞保护作用的分子基础。

关键词: 白藜芦醇, 细胞增殖, 基因组不稳定性, 细胞凋亡, 集落形成实验

Abstract: OBJECTIVE:To investigate the effects of resveratrol (RSV) on the genome instability (GIN) and proliferation of human colonic epithelial cell line NCM460 and colon cancer cell line SW620. METHODS:Effects of different concentrations of RSV (3.125,6.25,12.5,25,50,100 μmol/L) on cell proliferation were detected using the trypan blue exclusion method. CBMN-Cyt and colony formation experiments were performed to examine the effects of different concentrations of RSV (25,100 μmol/L) on GIN and clonal formation ability. RESULTS:Compared with the control group,RSV of 6.25-100 μmol/L significantly inhibited the proliferation of SW620 cells (P < 0.05). RSV of 100 μmol/L significantly increased the apoptosis rates,increased the GIN of SW620 cells and decreased the ability of clonal formation (P < 0.05 or 0.01). On the other hand,the same concentration of RSV decreased the GIN of NCM460 cells,enhanced cell division and cell clonality (all P < 0.05). CONCLUSION:The data show that RSV was able to maintain the genomic stability of the normal NCM460 epithelial cells but significantly increased the incidence of GIN in the SW620 colon cancer cells which could be the molecular basis for its excellent anticancer effect and normal cell protection in colon cancer therapy.

Key words: resveratrol, cell proliferation, genomic instability, apoptosis, colony formation assay

中图分类号: