癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (4): 289-294.doi: 10.3969/j.issn.1004-616x.2019.04.005

• 论著 • 上一篇    下一篇

肉苁蓉苯乙醇总苷脂质体对大鼠肝星状细胞增殖、凋亡及细胞周期的影响

张石蕾1, 由淑萍2, 马晓婷1, 马龙1, 赵军3, 刘涛1   

  1. 1. 新疆医科大学公共卫生学院卫生毒理学教研室, 新疆 乌鲁木齐 830011;
    2. 新疆医科大学护理学院基础护理学教研室, 新疆 乌鲁木齐 830011;
    3. 新疆维吾尔族自治区药物研究所, 新疆 乌鲁木齐 830004
  • 收稿日期:2018-06-06 修回日期:2019-03-15 出版日期:2019-07-30 发布日期:2019-08-23
  • 通讯作者: 刘涛,E-mail:xjmult@163.com E-mail:xjmult@163.com
  • 作者简介:张石蕾,E-mail:zhangsl6191@163.com。
  • 基金资助:
    国家自然科学基金资助项目(81560628)

Effect of Cistanche phenylethanol glycoside liposomes on proliferation,apoptosis and cell cycle of HSC cells in vitro

ZHANG Shilei1, YOU Shuping2, MA Xiaoting1, MA Long1, ZHAO Jun3, LIU tao1   

  1. 1. Department of Toxicology, College of Public Health, Xinjiang Medical University, Urumqi 830011;
    2. Department of Basic Nursing, College of Nursing, Xinjiang Medical University, Urumqi 830011;
    3. Xinjiang Uygur Autonomous Region Institute of Materia Medicine, Urumqi 830004, Xinjiang, China
  • Received:2018-06-06 Revised:2019-03-15 Online:2019-07-30 Published:2019-08-23

摘要: 目的:研究肉苁蓉苯乙醇总苷(CPhGs)脂质体对大鼠肝星状细胞(HSCs)增殖、凋亡、周期的影响及其相关分子机制。方法:HSCs分为空白对照组和CPhGs脂质体低、中、高浓度(分别为7.36、14.72、29.45 μg/mL)处理组。采用MTT法检测CPhGs脂质体对大鼠肝星状细胞增殖的影响;流式细胞术Annexin V-FITC/PI双染色法检测细胞凋亡状况,PI染色法检测大鼠肝星状细胞的周期变化;Western blot检测caspase-3、p27蛋白的表达变化。结果:与空白对照组比较,低、中、高3个剂量的CPhGs脂质体作用于HSCs后,均可抑制细胞的增殖(P < 0.05);3个剂量的CPhGs脂质体均可促使大鼠肝星状细胞发生凋亡,使细胞阻滞在G0/G1期,上调caspase-3及p27蛋白的表达(P < 0.05)。结论:CPhGs脂质体对体外培养大鼠肝星状细胞有抑制增殖、促进凋亡、阻滞细胞周期的作用。其机制可能与CPhGs脂质体上调caspase-3和p27蛋白表达有关。

关键词: 肉苁蓉苯乙醇总苷, 脂质体, 大鼠肝星状细胞, 增殖, 细胞凋亡, 细胞周期

Abstract: OBJECTIVE:To investigate the effect of phenylethanol glycoside liposomes from Cistanche (CPhG) on proliferation,apoptosis and cell cycle of HSC-T6. METHODS:HSC cultures were divided into untreated control and treated with low,medium and high concentrations of CPhG liposomes (7.36,14.72,29.45 μg/mL,respectively). Growth inhibition was detected by using the MTT assay,apoptosis was evaluated by Annexin V-FITC/PI staining and cell cycle changes were detected by flow cytometry. Protein expressions of cleaved caspase-3,p27 were detected using Western blotting. RESULTS:Compared with the control group,all treatment doses significantly inhibited cell proliferation and promoted apoptosis (P < 0.05). In addition,cells were arrested in the G0/G1 phase and showed significant up-regulated expression of caspase-3 and p27 proteins (P < 0.05). CONCLUSION:CPhG liposomes inhibited HSC cell proliferation,induced apoptosis and caused cell cycle arrest by up-regulating the protein expression of caspase-3 and p27.

Key words: Cistanche phenylethanoid glycosides, liposomes, HSC-T6, proliferation, apoptosis, cell cycle

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