肉苁蓉苯乙醇总苷脂质体对大鼠肝星状细胞增殖、凋亡及细胞周期的影响

doi:10.3969/j.issn.1004-616x.2019.04.005

癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (4): 289-294.doi: 10.3969/j.issn.1004-616x.2019.04.005

肉苁蓉苯乙醇总苷脂质体对大鼠肝星状细胞增殖、凋亡及细胞周期的影响

张石蕾¹, 由淑萍², 马晓婷¹, 马龙¹, 赵军³, 刘涛¹

1. 新疆医科大学公共卫生学院卫生毒理学教研室, 新疆乌鲁木齐 830011;
2. 新疆医科大学护理学院基础护理学教研室, 新疆乌鲁木齐 830011;
3. 新疆维吾尔族自治区药物研究所, 新疆乌鲁木齐 830004

收稿日期:2018-06-06 修回日期:2019-03-15 出版日期:2019-07-30 发布日期:2019-08-23
通讯作者: 刘涛,E-mail:xjmult@163.com E-mail:xjmult@163.com
作者简介:张石蕾,E-mail:zhangsl6191@163.com。
基金资助:
国家自然科学基金资助项目（81560628）

Effect of Cistanche phenylethanol glycoside liposomes on proliferation,apoptosis and cell cycle of HSC cells in vitro

ZHANG Shilei¹, YOU Shuping², MA Xiaoting¹, MA Long¹, ZHAO Jun³, LIU tao¹

1. Department of Toxicology, College of Public Health, Xinjiang Medical University, Urumqi 830011;
2. Department of Basic Nursing, College of Nursing, Xinjiang Medical University, Urumqi 830011;
3. Xinjiang Uygur Autonomous Region Institute of Materia Medicine, Urumqi 830004, Xinjiang, China

Received:2018-06-06 Revised:2019-03-15 Online:2019-07-30 Published:2019-08-23

摘要/Abstract

摘要： 目的：研究肉苁蓉苯乙醇总苷（CPhGs）脂质体对大鼠肝星状细胞（HSCs）增殖、凋亡、周期的影响及其相关分子机制。方法：HSCs分为空白对照组和CPhGs脂质体低、中、高浓度（分别为7.36、14.72、29.45 μg/mL）处理组。采用MTT法检测CPhGs脂质体对大鼠肝星状细胞增殖的影响；流式细胞术Annexin V-FITC/PI双染色法检测细胞凋亡状况，PI染色法检测大鼠肝星状细胞的周期变化；Western blot检测caspase-3、p27蛋白的表达变化。结果：与空白对照组比较，低、中、高3个剂量的CPhGs脂质体作用于HSCs后，均可抑制细胞的增殖（P < 0.05）；3个剂量的CPhGs脂质体均可促使大鼠肝星状细胞发生凋亡，使细胞阻滞在G0/G1期，上调caspase-3及p27蛋白的表达（P < 0.05）。结论：CPhGs脂质体对体外培养大鼠肝星状细胞有抑制增殖、促进凋亡、阻滞细胞周期的作用。其机制可能与CPhGs脂质体上调caspase-3和p27蛋白表达有关。

关键词: 肉苁蓉苯乙醇总苷, 脂质体, 大鼠肝星状细胞, 增殖, 细胞凋亡, 细胞周期

Abstract: OBJECTIVE:To investigate the effect of phenylethanol glycoside liposomes from Cistanche (CPhG) on proliferation,apoptosis and cell cycle of HSC-T6. METHODS:HSC cultures were divided into untreated control and treated with low,medium and high concentrations of CPhG liposomes (7.36,14.72,29.45 μg/mL,respectively). Growth inhibition was detected by using the MTT assay,apoptosis was evaluated by Annexin V-FITC/PI staining and cell cycle changes were detected by flow cytometry. Protein expressions of cleaved caspase-3,p27 were detected using Western blotting. RESULTS:Compared with the control group,all treatment doses significantly inhibited cell proliferation and promoted apoptosis (P < 0.05). In addition,cells were arrested in the G0/G1 phase and showed significant up-regulated expression of caspase-3 and p27 proteins (P < 0.05). CONCLUSION:CPhG liposomes inhibited HSC cell proliferation,induced apoptosis and caused cell cycle arrest by up-regulating the protein expression of caspase-3 and p27.

Key words: Cistanche phenylethanoid glycosides, liposomes, HSC-T6, proliferation, apoptosis, cell cycle

中图分类号:

R322.47

张石蕾, 由淑萍, 马晓婷, 马龙, 赵军, 刘涛. 肉苁蓉苯乙醇总苷脂质体对大鼠肝星状细胞增殖、凋亡及细胞周期的影响[J]. 癌变·畸变·突变, 2019, 31(4): 289-294.

ZHANG Shilei, YOU Shuping, MA Xiaoting, MA Long, ZHAO Jun, LIU tao. Effect of Cistanche phenylethanol glycoside liposomes on proliferation,apoptosis and cell cycle of HSC cells in vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 289-294.

参考文献

[1] 胡潇, 胡光荣, 刘思达, 等. 肝纤维化治疗进展[J]. 肝脏, 2018, 23(10):931-933.
[2] SUN H, CHEN G, WEN B, et al. Oligo-peptide I-C-F-6 inhibits hepatic stellate cell activation and ameliorates CCl4-induced liver fibrosis by suppressing NF-kappaB signaling and Wnt/beta-catenin signaling[J]. J Pharmacol Sci, 2018, 136(3):133-141
[3] 曾克武, 廖理曦, 万彦军, 等. 基于靶点"钩钓"策略的肉苁蓉苯乙醇苷药理靶点鉴定及功效解析[J]. 中草药, 2018, 49(1):173-178.
[4] 由淑萍, 赵军, 马龙, 等. 肉苁蓉苯乙醇总苷及其单体对肝星状细胞增殖和凋亡的影响[J]. 癌变·畸变·突变, 2017, 29(1):13-17.
[5] DESU H R, THOMA L A, WOOD G C. Nebulization of cyclic arginine-glycine-(D)-aspartic acid-peptide grafted and drug encapsulated liposomes for inhibition of acute lung injury[J/OL]. Pharm Res, 2018. doi:10.1007/s11095-018-2366-9.
[6] MATHEUS A D S R, PATRíCIA B D S, LARISSA S, et al. Nanotechnology-based drug delivery systems for control of microbial biofilms:a review[J]. Int J Nanomedicine, 2018, 13:1179-1213.
[7] 由淑萍, 赵军, 马龙, 等. 肉苁蓉乙醇提取物抗大鼠免疫性肝纤维化的作用及其机制[J]. 中国药理学与毒理学杂志, 2016, 30(5):504-510.
[8] WILKINS M, HALL-STOODLEY L, ALLAN R N, et al. New approaches to the treatment of biofilm-related infections[J]. J Infect, 2014, 69(1):47-52.
[9] DESU H R, WOOD G C, THOMA L A. Non-invasive detection of lung inflammation by near-infrared fluorescence imaging using bimodal liposomes[J]. J Fluoresc, 2016, 26(1):241-253.
[10] MIRALBELL J, LóPEZ-CANCIO E, LóPEZ-OLORIZ J, et al. Cognitive patterns in relation to biomarkers of cerebrovascular disease and vascular risk factors[J]. Cerebrovasc Dis, 2013, 36(2):98-105.
[11] -PERJU-DUMBRAVA L, MUNTEAN M L, FIOR M D. Cerebrovascular profile assessment in Parkinson's disease patients[J]. CNS Neurol Disord Drug Targets, 2014, 13(4):712-717.
[12] YOU S P, MA L, ZHAO J, et al. Phenylethanol glycosides from cistanche tubulosa suppress hepatic stellate cell activation and block the conduction of signaling pathways in TGF-beta1/smad as potential anti-hepatic fibrosis agents[J/OL]. Molecules, 2016, 21(1):102. doi:10.3390/molecules21010102.
[13] 秦棪楠, 钟耀刚, 李铮. 人肝星状细胞激活和抑制及其相关分子机制的研究进展[J]. 中国细胞生物学学报, 2011, 33(10):1127-1136.
[14] ABBATE I, DIANZANI F F M R, CAPOBIANCHI M R. Activation of signal transduction and apoptosis in healthy lymphomonocytes exposed to bystander HIV-1-infected cells[J]. J Clin Exp Immunol, 2000, 122(3):374-380.
[15] 周瑞娟, 陈红风, 叶媚娜, 等. 芒柄花素对不同亚型乳腺癌细胞周期基因和蛋白表达的影响[J]. 药物评价研究, 2016, 39(3):362-366.
[16] 周瑞娟, 陈红风, 叶媚娜, 等. 芒柄花素对不同亚型乳腺癌细胞增殖及细胞周期的影响[J]. 肿瘤防治研究, 2012, 39(9):1051-1055.
[17] RUGGIERI A, TRANI L D, GATTO I, et al. Canine coronavirus induces apoptosis in cultured cells[J]. Vet Microbiol, 2007, 121(1/2):64-72.

肉苁蓉苯乙醇总苷脂质体对大鼠肝星状细胞增殖、凋亡及细胞周期的影响

Effect of Cistanche phenylethanol glycoside liposomes on proliferation,apoptosis and cell cycle of HSC cells in vitro

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