癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (6): 479-482.doi: 10.3969/j.issn.1004-616x.2019.06.012

• 检测研究 • 上一篇    下一篇

甲基丙烯酸环氧丙酯的遗传毒性评价

王全凯1,3, 谢广云1,3, 马顺鹏1,3, 郭浩然2, 乌瀚宝栎尔1, 宋佳阳1, 许建宁1,3   

  1. 1. 中国疾病预防控制中心职业卫生与中毒控制所, 北京 100050;
    2. 江阴市疾病预防控制中心, 江苏 无锡 214434;
    3. 中国疾病预防控制中心化学污染与健康安全重点实验室, 北京 100050
  • 收稿日期:2019-08-22 修回日期:2019-11-06 出版日期:2019-11-30 发布日期:2019-12-04
  • 通讯作者: 许建宁,E-mail:jnx999@263.net E-mail:jnx999@263.net
  • 作者简介:王全凯,E-mail:kylewang@sina.com。
  • 基金资助:
    国家自然科学基金资助项目(81673221)

Genotoxicity evaluation of glycidyl methacrylate

WANG Quankai1,3, XIE Guangyun1,3, MA Shunpeng1,3, GUO Haoran2, WUHAN Baolier1, SONG Jiayang1, XU Jianning1,3   

  1. 1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050;
    2. Jiangyin Municipal Center for Disease Control and Prevention, Wuxi 214434, Jiangsu;
    3. Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
  • Received:2019-08-22 Revised:2019-11-06 Online:2019-11-30 Published:2019-12-04

摘要: 目的:观察甲基丙烯酸环氧丙酯(GMA)诱发体外培养的中国仓鼠肺细胞(V79)微核发生变化情况,对其遗传毒性进行评价。方法:分别采用不同浓度(2.25、4.5、9.0、18.0、36.0 μg/mL)的GMA染毒V79细胞,设置空白对照组和DMSO溶剂对照组,其中染毒3 h处理组分别在加和不加体外活化系统(S9)条件下进行,染毒24 h处理组在不加S9条件下进行。分别计算细胞复制指数和微核细胞率。结果:GMA的浓度在2.25~36.0 μg/mL范围内,无S9的条件下,与空白对照组和DMSO溶剂对照组相比,GMA染毒3和24 h组的V79细胞复制指数均无明显变化,但微核细胞率明显升高,并呈浓度-效应关系;在有S9的条件下染毒3 h,各剂量组的GMA诱发微核细胞率的差异均无统计学意义。结论:在2.25~36.0 μg/mL浓度范围,GMA可致V79细胞的微核率升高,表明GMA可诱发遗传物质损伤,具有遗传毒性。

关键词: 甲基丙烯酸环氧丙酯, 中国仓鼠肺细胞, 微核试验, 遗传毒性

Abstract: OBJECTIVE: To evaluate genotoxicity of glycidyl methacrylate (GMA) in Chinese hamster lung cells (V79) using the micronucleus assay. METHODS: V79 cells were treated with different doses of GMA (2.25,4.5,9.0,18.0,36.0 μg/mL) for 3 h with or without an in vitro activation system (S9). Controls include a blank and a DMSO group. Another treatment group was treated for 24 h without S9. Cell replication indices and binuclear micronucleus rates were determined. RESULTS: Without S9,the replication index of the treated V79 cells did not change significantly but the incidence of micronuclei in binuclear cells increased significantly in a dose-dependent manner. With the addition of S9,the rate of micronucleated cells in the high-dose group was not statistically significant. CONCLUSION: In the concentration range of 2.25-36.0 μg/mL,GMA induced micronuclei in V79 cells,indicating that GMA is genotoxic.

Key words: glycidyl methacrylate, Chinese hamster lung cells, micronucleus test, genotoxicity

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