深圳和太原PM2.5样品致突变作用研究

doi:10.3969/j.issn.1004-616x.2019.06.013

癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (6): 483-487.doi: 10.3969/j.issn.1004-616x.2019.06.013

深圳和太原PM_2.5样品致突变作用研究

郑凯^1,2, 王冰玉^1,2, 徐新云¹, 耿红³, 黄海燕¹, 刘威¹, 龙鼎新²

1. 深圳市疾病预防控制中心环境与健康所, 广东深圳 518055;
2. 南华大学公共卫生学院, 湖南衡阳 421001;
3. 山西大学环境科学研究所, 山西太原 030006

收稿日期:2019-03-27 修回日期:2019-10-21 出版日期:2019-11-30 发布日期:2019-12-04
通讯作者: 徐新云,E-mail:xyxu2008@163.com E-mail:xyxu2008@163.com
作者简介:郑凯,E-mail:1015265323@qq.com。
基金资助:
深圳市科技研发学科布局项目（JCYJ20170413101713324）；山西省重点研发计划项目（201803D31025）

Mutagenicity of PM_2.5 samples from Shenzhen and Taiyuan

ZHENG Kai^1,2, WANG Bingyu^1,2, XU Xinyun¹, GENG Hong³, HUANG Haiyan¹, LIU Wei¹, LONG Dingxin²

1. Institue of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong;
2. School of Public Health, University of South China, Hengyang 421001, Hunan;
3. Institute of Environmental Science, Shanxi University, Taiyuan 030006, Shanxi, China

Received:2019-03-27 Revised:2019-10-21 Online:2019-11-30 Published:2019-12-04

摘要/Abstract

摘要： 目的：探讨深圳和太原大气细颗粒物（PM_2.5）样品是否具有致突变作用。方法：应用组氨酸缺陷型鼠伤寒沙门氏菌TA97、TA98、TA100、TA102和TA1535，通过平板渗入法同时对广东深圳和山西太原两地的PM_2.5样品进行标准Ames试验。实验设4个PM_2.5剂量组（分别为每皿40、100、200和400 μg）、1个阴性对照组和1个阳性对照组，每种细菌均设立加S9和不加S9两种试验组。结果：深圳PM_2.5样品的Ames试验结果显示，TA97、TA100和TA102在PM_2.5样品处理后菌落回变数显著高于阴性对照组，加S9与不加S9的菌落回变数比较差异有统计学意义（P < 0.01）。太原样品的Ames试验结果显示，TA97、TA98和TA100在PM_2.5样品处理后菌落回变数显著高于阴性对照组，加S9与不加S9的菌落回变数比较差异有统计学意义（P < 0.05或P < 0.01）。但深圳和太原PM_2.5样品对TA1535未见阳性结果，结论：深圳和太原PM_2.5样品均能够引起TA97、TA98、TA100的Ames试验的自发回变数阳性结果，表明深圳和太原PM_2.5样品均具有致基因突变作用。

关键词: 大气细颗粒物, Ames试验, 自发突变菌落, 致突变性

Abstract: OBJECTIVE: The Ames tests were used to investigate the mutagenicity of PM_2.5 samples which were collected from Shenzhen and Taiyuan. METHODS: The histidine-deficient Salmonella typhimurium strains TA97,TA98,TA100,TA102 and TA1535 were used. From the collected samples,four doses of PM_2.5 (40,100,200,and 400 μg per dish),a negative control and a positive control were tested. Each test group was treated with S9 or without S9. RESULTS: After treatment with PM_2.5 samples from Shenzhen,there were significant differences in mutagenicity (P < 0.01) between +S9 and -S9 groups in TA98,TA100 and TA102.Similar results were observed with PM_2.5 samples from Taiyuan in TA97,TA98 and TA100. These results indicated that the PM_2.5 samples from Shenzhen and Taiyuan contained mutagenic chemicals which did not cause mutation in TA1535. CONCLUSION: Overall,our results indicate that PM_2.5 samples from Shenzhen and Taiyuan contained directly and indirectly acting mutagens.

Key words: PM_2.5, Ames test, spontaneous mutation colonies, mutagenicity

中图分类号:

R122.7

郑凯, 王冰玉, 徐新云, 耿红, 黄海燕, 刘威, 龙鼎新. 深圳和太原PM_2.5样品致突变作用研究[J]. 癌变·畸变·突变, 2019, 31(6): 483-487.

ZHENG Kai, WANG Bingyu, XU Xinyun, GENG Hong, HUANG Haiyan, LIU Wei, LONG Dingxin. Mutagenicity of PM_2.5 samples from Shenzhen and Taiyuan[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 483-487.

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深圳和太原PM_2.5样品致突变作用研究

Mutagenicity of PM_2.5 samples from Shenzhen and Taiyuan

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深圳和太原PM2.5样品致突变作用研究

Mutagenicity of PM2.5 samples from Shenzhen and Taiyuan

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深圳和太原PM_2.5样品致突变作用研究

Mutagenicity of PM_2.5 samples from Shenzhen and Taiyuan