癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (6): 483-487.doi: 10.3969/j.issn.1004-616x.2019.06.013

• 检测研究 • 上一篇    下一篇

深圳和太原PM2.5样品致突变作用研究

郑凯1,2, 王冰玉1,2, 徐新云1, 耿红3, 黄海燕1, 刘威1, 龙鼎新2   

  1. 1. 深圳市疾病预防控制中心环境与健康所, 广东 深圳 518055;
    2. 南华大学公共卫生学院, 湖南 衡阳 421001;
    3. 山西大学环境科学研究所, 山西 太原 030006
  • 收稿日期:2019-03-27 修回日期:2019-10-21 出版日期:2019-11-30 发布日期:2019-12-04
  • 通讯作者: 徐新云,E-mail:xyxu2008@163.com E-mail:xyxu2008@163.com
  • 作者简介:郑凯,E-mail:1015265323@qq.com。
  • 基金资助:
    深圳市科技研发学科布局项目(JCYJ20170413101713324);山西省重点研发计划项目(201803D31025)

Mutagenicity of PM2.5 samples from Shenzhen and Taiyuan

ZHENG Kai1,2, WANG Bingyu1,2, XU Xinyun1, GENG Hong3, HUANG Haiyan1, LIU Wei1, LONG Dingxin2   

  1. 1. Institue of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong;
    2. School of Public Health, University of South China, Hengyang 421001, Hunan;
    3. Institute of Environmental Science, Shanxi University, Taiyuan 030006, Shanxi, China
  • Received:2019-03-27 Revised:2019-10-21 Online:2019-11-30 Published:2019-12-04

摘要: 目的:探讨深圳和太原大气细颗粒物(PM2.5)样品是否具有致突变作用。方法:应用组氨酸缺陷型鼠伤寒沙门氏菌TA97、TA98、TA100、TA102和TA1535,通过平板渗入法同时对广东深圳和山西太原两地的PM2.5样品进行标准Ames试验。实验设4个PM2.5剂量组(分别为每皿40、100、200和400 μg)、1个阴性对照组和1个阳性对照组,每种细菌均设立加S9和不加S9两种试验组。结果:深圳PM2.5样品的Ames试验结果显示,TA97、TA100和TA102在PM2.5样品处理后菌落回变数显著高于阴性对照组,加S9与不加S9的菌落回变数比较差异有统计学意义(P < 0.01)。太原样品的Ames试验结果显示,TA97、TA98和TA100在PM2.5样品处理后菌落回变数显著高于阴性对照组,加S9与不加S9的菌落回变数比较差异有统计学意义(P < 0.05或P < 0.01)。但深圳和太原PM2.5样品对TA1535未见阳性结果,结论:深圳和太原PM2.5样品均能够引起TA97、TA98、TA100的Ames试验的自发回变数阳性结果,表明深圳和太原PM2.5样品均具有致基因突变作用。

关键词: 大气细颗粒物, Ames试验, 自发突变菌落, 致突变性

Abstract: OBJECTIVE: The Ames tests were used to investigate the mutagenicity of PM2.5 samples which were collected from Shenzhen and Taiyuan. METHODS: The histidine-deficient Salmonella typhimurium strains TA97,TA98,TA100,TA102 and TA1535 were used. From the collected samples,four doses of PM2.5 (40,100,200,and 400 μg per dish),a negative control and a positive control were tested. Each test group was treated with S9 or without S9. RESULTS: After treatment with PM2.5 samples from Shenzhen,there were significant differences in mutagenicity (P < 0.01) between +S9 and -S9 groups in TA98,TA100 and TA102.Similar results were observed with PM2.5 samples from Taiyuan in TA97,TA98 and TA100. These results indicated that the PM2.5 samples from Shenzhen and Taiyuan contained mutagenic chemicals which did not cause mutation in TA1535. CONCLUSION: Overall,our results indicate that PM2.5 samples from Shenzhen and Taiyuan contained directly and indirectly acting mutagens.

Key words: PM2.5, Ames test, spontaneous mutation colonies, mutagenicity

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