癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (1): 29-32.doi: 10.3969/j.issn.1004-616x.2020.01.005

• 论著 • 上一篇    下一篇

哈萨克族食管癌组织中miR-143、miR-145和Survivin mRNA的表达及其临床病理意义

封敏1, 艾比拜·莫合塔尔2, 石静怡2, 祖可来·艾力江2, 李永祥2, 闫长顺2, 李秀梅3   

  1. 1. 新疆医科大学附属肿瘤医院, 新疆 乌鲁木齐 830011;
    2. 新疆医科大学临床医学专业, 新疆 乌鲁木齐 830011;
    3. 新疆医科大学基础医学院, 新疆 乌鲁木齐 830011
  • 收稿日期:2018-11-12 修回日期:2019-09-16 出版日期:2020-01-31 发布日期:2020-02-05
  • 通讯作者: 李秀梅,E-mail:dr.lxm@163.com E-mail:dr.lxm@163.com
  • 作者简介:封敏,E-mail:834454503@qq.com。
  • 基金资助:
    新疆维吾尔自治区自然科学基金项目(2017D01C392)

Expression of miR-143, miR-145 and Survivin mRNA in esophageal cancers among Kazaks

FENG Min1, ABBIE·Mohetar2, SHI Jingyi2, ZUKELAI·Alijiang2, LI Yongxiang2, YAN Changshun2, LI Xiumei3   

  1. 1. Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011;
    2. Clinical Medicine, Xinjiang Medical University, Urumqi 830011;
    3. School of Basic Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang, China
  • Received:2018-11-12 Revised:2019-09-16 Online:2020-01-31 Published:2020-02-05

摘要: 目的:检测新疆哈萨克族食管癌组织中miR-143、miR-145和Survivin mRNA的表达,探讨其在哈萨克族食管癌发生发展中的作用及临床意义。方法:采用实时荧光定量PCR方法检测47例哈萨克族食管癌及癌旁组织标本中miR-143、miR-145和Survivin mRNA的表达水平,分析它们与临床病理指标的关系。结果:miR-143、miR-145在哈萨克族食管癌组织中表达较癌旁组织降低(P均 < 0.01);Survivin mRNA在哈萨克族食管癌组织中表达较癌旁组织升高(P=0.000);miR-143的表达与肿瘤分化程度、淋巴结转移、临床分期相关(P=0.018、0.004、0.022);miR-145的表达与分化程度、淋巴结转移相关(P=0.007、0.039);Survivin mRNA的表达与淋巴结转移及临床分期相关(P=0.042、0.034)。miR-143的表达与miR-145呈正相关(r=0.662,P=0.000),与Survivin mRNA呈负相关(r=-0.313,P=0.002);结论:在哈萨克族食管癌中miR-143、miR-145低表达、Survivin mRNA高表达,miR-143/145基因簇、Survivin mRNA可能共同参与哈萨克族食管癌发生发展过程。

关键词: 食管癌, miR-143, miR-145, Survivin, 哈萨克族

Abstract: OBJECTIVE: To explore the expression of miR-143,miR-145 and Survivin mRNAs,and their clinical significance in esophageal cancers among Kazaks in Xinjiang. METHODS: Expression levels of miR-143,miR-145 and Survivin mRNAs in 47 cases of Kazak esophageal cancer were detected by qPCR. RESULTS: The expression of miR-143 and miR-145 in cancer tissues was lower than that in the distal non-cancerous tissues,and the difference was significant (P < 0.01). On the other hand,survivin expression in cancer tissues was higher than that in the distal non-cancerous tissues (P=0.000). Expression of miR-143 was correlated with degree of differentiation,lymph node metastasis and clinical stages (P=0.018,0.004,0.022,respectively). Expression of miR-145 was correlated with degree of differentiation and lymph node metastasis (P=0.007,0.039,respectively). Expression of Survivin mRNA was correlated with lymph node metastasis and clinical stages (P=0.042,0.034,respectively). Expression of miR-143 was positively correlated with miR-145 (r=0.662,P=0.000),and negatively correlated with Survivin mRNA (r=-0.313,P=0.002). CONCLUSION: miR-143,miR-145,and Survivin mRNA are involved in the development and progression of Kazak esophageal cancer.

Key words: esophageal cancer, miR-143, miR-145, Survivin, Kazak

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