邻苯二甲酸二(2-乙基)己酯长期暴露对成年雄鼠甲状腺激素水平的影响

doi:10.3969/j.issn.1004-616x.2020.01.009

癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (1): 47-51.doi: 10.3969/j.issn.1004-616x.2020.01.009

邻苯二甲酸二(2-乙基)己酯长期暴露对成年雄鼠甲状腺激素水平的影响

张婉莹, 吴昊宇, 董新文, 苗新秀楠, 冀俊昌, 王占举, 那晓琳, 张云波

哈尔滨医科大学公共卫生学院环境卫生教研室, 黑龙江哈尔滨 150081

收稿日期:2018-11-22 修回日期:2019-12-30 出版日期:2020-01-31 发布日期:2020-02-05
通讯作者: 那晓琳,E-mail:naxiaolin1495@sohu.com;张云波,E-mail:zhangyunbo1982@126.com E-mail:naxiaolin1495@sohu.com;zhangyunbo1982@126.com
作者简介:张婉莹,E-mail:1187636092@qq.com。
基金资助:
国家自然科学基金项目（81273079）

Effects of long-term exposure to DEHP on thyroid hormone levels in adult male mice

ZHANG Wanying, WU Haoyu, DONG Xinwen, MIAO Xinxiunan, JI Junchang, WANG Zhanju, NA Xiaolin, ZHANG Yunbo

Department of Environmental Hygiene, Public Health College, Harbin Medical University, Harbin 150081, Heilongjiang, China

Received:2018-11-22 Revised:2019-12-30 Online:2020-01-31 Published:2020-02-05

摘要/Abstract

摘要： 目的：探讨邻苯二甲酸二（2-乙基）己酯（DEHP）长期暴露对成年雄性大鼠甲状腺激素水平产生的影响。方法：将36只健康的成年雄性Wistar大鼠按体质量随机分为4组，分别为对照组以及150、300、600 mg/kg DEHP暴露组。对照组大鼠正常饮食和饮水，暴露组大鼠连续灌胃给予DEHP 3个月。处死大鼠前称取大鼠体质量并计算各组织脏体比，检测尿碘含量，血清甲状腺激素含量及血清TTR、TRH、TSAb水平。采用单因素方差分析法分析各指标的组间差异，进一步的多重比较采用SNK检验。结果：经过3个月的DEHP暴露，大鼠体质量无明显变化。与对照组相比，各剂量组大鼠甲状腺湿重和脏体比无显著性差异。150、300、600 mg/kg剂量组大鼠肝脏脏体比增加并呈剂量-效应关系（P < 0.05）。600 mg/kg剂量组大鼠尿碘水平显著降低（P < 0.05）。600 mg/kg剂量组血清FT4（血清游离甲状腺素）水平降低（P < 0.05）。结论：DEHP 3个月的暴露期对大鼠体质量未产生明显影响，对甲状腺、肝脏脏器系统功能产生一定的影响。DEHP的暴露可抑制大鼠体内TSH的合成、分泌及转运，扰乱甲状腺激素水平。

关键词: 环境内分泌干扰物, 邻苯二甲酸二(2-乙基)己酯, 甲状腺疾病, 甲状腺激素, 雄鼠

Abstract: OBJECTIVE: To investigate effects of long-term exposure to di-(2-ethylhexyl) phthalate (DEHP) on thyroid hormone levels in adult male rats. METHODS: Thirty-six healthy adult male Wistar rats were randomly divided into four groups according to body weight:control group and the 150,300 and 600 mg/kg DEHP exposure group. Rats were given normal food and water,but the rats in the exposed group were given DEHP by continuous gavage for 3 months. Before the rats were sacrificed,body masses of the rats were measured and the viscera ratio of each tissue was calculated. Urine iodine content,serum thyroid hormone content and serum TTR,TRH and TSAb levels were detected. One-way analysis of variance (ANOVA) was used to analyze the differences among the groups. SNK test was used for further multiple comparisons. RESULTS: After 3 months of exposure to DEHP,there was no significant change in body mass. Compared with the control group,there was no significant difference in the wet weight of thyroid and the ratio of viscera in each dose group. The ratios of liver viscera in the 150,300 and 600 mg/kg groups increased and showed a dose-effect relationship (P < 0.05). Urine iodine level of rats in the 600 mg/kg group was significantly decreased (P < 0.05). Serum FT4 level of rats in the 600 mg/kg group was decreased (P < 0.05). CONCLUSION: Exposure of rats to DEHP for 3 months had no significant effect on the body masses of rats. However,the exposure affected the function of thyroid and liver organs via inhibiting the synthesis,secretion and transport of TSH,and disrupting the thyroid hormone levels.

Key words: endocrine disrupting chemicals, DEHP, thyroid disease, thyroid hormone, male rats

中图分类号:

R994.6

张婉莹, 吴昊宇, 董新文, 苗新秀楠, 冀俊昌, 王占举, 那晓琳, 张云波. 邻苯二甲酸二(2-乙基)己酯长期暴露对成年雄鼠甲状腺激素水平的影响[J]. 癌变·畸变·突变, 2020, 32(1): 47-51.

ZHANG Wanying, WU Haoyu, DONG Xinwen, MIAO Xinxiunan, JI Junchang, WANG Zhanju, NA Xiaolin, ZHANG Yunbo. Effects of long-term exposure to DEHP on thyroid hormone levels in adult male mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 47-51.

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邻苯二甲酸二(2-乙基)己酯长期暴露对成年雄鼠甲状腺激素水平的影响

Effects of long-term exposure to DEHP on thyroid hormone levels in adult male mice

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