癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (3): 166-170,176.doi: 10.3969/j.issn.1004-616x.2020.03.002

• 论著 • 上一篇    下一篇

基于代谢组学方法筛选大鼠血浆辐射敏感代谢物

赵骅, 陆雪, 蔡恬静, 李爽, 田梅, 刘青杰   

  1. 中国疾病预防控制中心辐射防护与核安全医学所, 辐射防护与核应急中国疾病预防控制中心重点实验室, 北京 100088
  • 收稿日期:2020-01-09 修回日期:2020-03-31 出版日期:2020-05-31 发布日期:2020-06-03
  • 通讯作者: 刘青杰,E-mail:liuqingjie@nirp.chinacdc.cn E-mail:liuqingjie@nirp.chinacdc.cn
  • 作者简介:赵骅,E-mail:zhaohua@nirp.chinacdc.cn。
  • 基金资助:
    国家自然科学基金(81573081)

Screening for radiation-sensitive biomarkers in rat plasma based on metabolomics studies

ZHAO Hua, LU Xue, CAI Tianjing, LI Shuang, TIAN Mei, LIU Qingjie   

  1. National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, Beijing 100088, China
  • Received:2020-01-09 Revised:2020-03-31 Online:2020-05-31 Published:2020-06-03

摘要: 目的:筛选大鼠血浆辐射敏感代谢物并探索其代谢通路,为辐射损伤生物标志物研究提供科学依据。方法:60Co γ射线对20只大鼠进行全身照射,照射剂量分别为0、1、3、5 Gy,剂量率为1 Gy/min,采用基于液相色谱质谱串联平台的非靶向代谢组学方法,检测大鼠受照7 d后血浆中代谢物的变化。结果:大鼠受照后,筛选出血浆中11个辐射敏感代谢物,主要涉及牛磺酸和亚牛磺酸代谢、烟酸和烟酰胺代谢、初级胆汁酸生物合成等代谢通路。十八二烯酰基肉碱、十八一烯酰基肉碱、十六酰基肉碱、牛磺酸、L-犬尿氨酸及胞嘧啶随受照剂量的上升而显著下降(P < 0.05),具有明显的剂量-效应关系。结论:大鼠受到γ射线照射后,血浆中代谢物水平发生显著变化,有6个代谢物具备成为辐射生物剂量计的基础条件。

关键词: 代谢组学, 电离辐射, 生物标志物, 剂量效应

Abstract: OBJECTIVE: To screen for radiation-sensitive metabolites in rat plasma and to explore the metabolic pathways that provide scientific basis for identification of radiation-sensitive biomarkers. METHODS: Rats irradiated whole-body with 60Co gamma rays of 0,1,3 and 5 Gy,respectively. Changes of metabolites in rat plasma were detected by non-targeted metabolomics method based on the liquid chromatography mass spectrometry platform. RESULTS: Eleven plasma metabolites were identified as the potential radiation biomarkers. Taurine and hypotaurine metabolism,nicotinic acid and nicotinamide metabolism,and primary bile acid biosynthesis were enriched after irradiation. Linoelaidylcarnitine,11Z-octadecenylcarnitine,L-palmitoylcarnitine,taurine,niacinamide,L-kynurenine and cytosine were decreased with the increased dose and had good dose-response relationships. CONCLUSION: The metabolites levels in rat plasma were significantly changed after exposure to gamma-rays. Six metabolites had the basic conditions for radiation biological dosimeters.

Key words: metabolomics, ionizing radiation, biomarker, dose response

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