中药地锦草乙醇提取物体外抑制胃癌细胞增殖活性及机制研究

doi:10.3969/j.issn.1004-616x.2020.03.006

癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (3): 187-193.doi: 10.3969/j.issn.1004-616x.2020.03.006

中药地锦草乙醇提取物体外抑制胃癌细胞增殖活性及机制研究

王耀杰¹, 相晓晗¹, 韩丽娜¹, 李军², 霍炳杰³, 赵连梅¹

1. 河北医科大学第四医院科研中心, 河北石家庄 050011;
2. 河北医科大学第四医院药学部, 河北石家庄 050011;
3. 河北医科大学第四医院中医科, 河北石家庄 050011

收稿日期:2019-07-23 修回日期:2020-04-19 出版日期:2020-05-31 发布日期:2020-06-03
通讯作者: 赵连梅,E-mail:lianmeizhmail@163.com E-mail:lianmeizhmail@163.com
作者简介:王耀杰,E-mail:1426649157@qq.com。
基金资助:
国家自然科学基金（81772550，81673642）

Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro

WANG Yaojie¹, XIANG Xiaohan¹, HAN Lina¹, LI Jun², HUO Bingjie³, ZHAO Lianmei¹

1. Research Center of the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011;
2. Pharmaceutical Department of the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011;
3. Department of Traditional Chinese Medicine of the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China

Received:2019-07-23 Revised:2020-04-19 Online:2020-05-31 Published:2020-06-03

摘要/Abstract

摘要： 目的：探讨地锦草乙醇提取物（EEEH）对人胃癌（GC）细胞BGC823、MGC803、SGC7901及AGS增殖活性的抑制作用及机制。方法：采用蒸馏和冻干技术制备EEEH，用不同浓度的EEEH（62.5、125、250和500 μg/mL）处理GC细胞，并设置溶剂对照组和空白对照组。采用MTS法检测胃癌细胞存活率；采用流式细胞术检测胃癌细胞凋亡率和周期分布；采用Western blot法检测胃癌细胞增殖、凋亡以及周期相关蛋白的表达水平。结果：MTS细胞增殖实验结果显示，与对照组相比，随着地锦草乙醇提取物浓度增加，GC细胞的增殖能力逐渐减弱，差异均有统计学意义（P < 0.05）；流式细胞术检测结果显示，62.5、125和250 μg/mL的EEEH处理GC细胞MGC803和AGS 24 h后，与对照组相比，GC细胞的凋亡率显著增加，差异均有统计学意义（P < 0.05），并且AGS细胞的细胞周期阻滞在G0/G1期（P均 < 0.05）；Western blot检测结果显示，与对照组相比，经EEEH处理过的胃癌细胞中增殖相关蛋白PCNA以及抗凋亡相关蛋白BCL-2和BCL-XL的蛋白表达水平显著降低，促凋亡相关蛋白caspase-8和caspase-3出现明显的活性裂解片段，表明经EEEH处理后胃癌细胞凋亡活性增强，差异均有统计学意义（P < 0.05），而caspase-9的蛋白表达水平并无明显变化。结论：EEEH能够抑制GC细胞增殖，其机制可能与EEEH诱导细胞凋亡和周期阻滞有关。

关键词: 地锦草乙醇提取物, 胃癌细胞, 增殖, 凋亡, 细胞周期

Abstract: OBJECTIVE: To investigate the inhibitory effects of ethanol extract from Euphorbia humifusa(EEEH) on activities of BGC823,MGC803,SGC7901 and AGS in human gastric cancer (GC) cells. METHODS: The EEEH was prepared by distillation and freeze-drying technology. The GC cells were treated with different concentrations of EEEH (0,62.5,125,250 and 500 μg/mL) and the solvent control. The cell survival rates were detected by the MTS method,the apoptosis rates and cell cycle distributions were detected by flow cytometry,and the protein expression levels of cell proliferation,apoptosis and cycle were detected by Western blot method. RESULTS: After treatment with different concentrations of EEEH,the proliferation of GC cells was significantly inhibited compared with the control group and the difference was statistically significant (P < 0.05). The results of flow cytometry show that the apoptosis rates of treated AGS cells increased significantly and the cell cycle was arrested in G0/G1 phase. The differences were significant from that of the control (P < 0.05). The results of Western blot test show that the expression levels of proliferation-related protein PCNA,anti-apoptosis-related protein BCL-2 and BCL-XL in the treated cells were significantly lower than those in the control group,and apoptosis-related protein caspase-8 and caspase-3 showed obvious lytic fragments,indicating increased activities. The differences were statistically significant (P < 0.05). However,there were no significant changes in the expression levels and activities of caspase-9 protein. CONCLUSION: EEEH inhibited GC cell proliferation via the induction of apoptosis and cycle arrest.

Key words: ethanol extract from Euphorbia humifusa, gastric cancer cell, proliferation, apoptosis, cell cycle

中图分类号:

R735.2

王耀杰, 相晓晗, 韩丽娜, 李军, 霍炳杰, 赵连梅. 中药地锦草乙醇提取物体外抑制胃癌细胞增殖活性及机制研究[J]. 癌变·畸变·突变, 2020, 32(3): 187-193.

WANG Yaojie, XIANG Xiaohan, HAN Lina, LI Jun, HUO Bingjie, ZHAO Lianmei. Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 187-193.

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中药地锦草乙醇提取物体外抑制胃癌细胞增殖活性及机制研究

Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro

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