癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (4): 275-280.doi: 10.3969/j.issn.1004-616x.2020.04.005

• 论著 • 上一篇    下一篇

RNA结合基元蛋白24通过调控HOTAIR表达抑制鼻咽癌细胞增殖

陈琪1, 钟茜2, 岳文涛1   

  1. 1. 首都医科大学附属北京妇产医院中心实验室, 北京 100026;
    2. 华南肿瘤学国家重点实验室/中山大学肿瘤防治中心/肿瘤医学协同创新中心, 广东 广州 510060
  • 收稿日期:2019-10-24 修回日期:2020-05-31 出版日期:2020-07-31 发布日期:2020-08-01
  • 通讯作者: 岳文涛,E-mail:yuewt@ccmu.edu.cn;钟茜,E-mail:zhongqian@sysucc.org.cn E-mail:yuewt@ccmu.edu.cn;zhongqian@sysucc.org.cn
  • 作者简介:陈琪,E-mail:yqglzhh@163.com。
  • 基金资助:
    国家自然科学基金(81502353,81672838);北京市医院管理局临床技术创新项目(XMLX201705);首都医科大学附属北京妇产医院中青年学科骨干培养专项(FCYY201713)

Inhibition of nasopharyngeal carcinoma proliferation by RBM24 via regulation of HOTAIR

CHEN Qi1, ZHONG Qian2, YUE Wentao1   

  1. 1. Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026;
    2. State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Collaborative Innovation Center of Cancer Medicine, Guangzhou 510060, Guangdong, China
  • Received:2019-10-24 Revised:2020-05-31 Online:2020-07-31 Published:2020-08-01

摘要: 目的: 探讨RNA结合基元蛋白24(RBM24)抑制鼻咽癌细胞增殖的可能机制。方法: 分别在永生化鼻咽上皮细胞N5、NP69和鼻咽癌细胞CNE1中转染RBM24 siRNA构建敲低RBM24表达的细胞模型。建模后分别于1~5 d用CCK-8法检测细胞增殖活性,用实时荧光PCR法检测细胞HOX转录反义RNA(HOTAIR)的表达水平。结果: 实时荧光PCR法检测结果表明敲低RBM24表达的细胞模型构建成功。第4天时RBM24敲低组的CNE1、N5细胞增殖率(分别为5.11±0.03和2.09±0.18)与相应的对照组细胞(分别为4.53±0.05和1.73±0.12)相比均升高(P均 < 0.05),且CNE1、N5细胞的HOTAIR mRNA表达水平(分别为67.54±1.87和7.81±1.90)较相应的对照组(1.00±0.21和1.00±0.19)亦升高,差异均有统计学意义(P均 < 0.05),而NP69细胞的增殖率和HOTAIR mRNA表达水平变化不明显(P均 > 0.05)。结论: RBM24可抑制鼻咽癌CNE1细胞和永生化鼻咽上皮N5细胞的增殖,其机制可能是通过抑制HOTAIR表达起作用。

关键词: RNA结合基元蛋白24, 鼻咽癌, 永生化鼻咽上皮细胞, HOX转录反义RNA, 增殖

Abstract: OBJECTIVE: This study aimed to explore mechanisms of RBM24 in inhibition of proliferation in nasopharyngeal carcinoma cells. METHODS: Cell models with RBM24 knocked down were built via RBM24 siRNA transfection into immortalized nasopharyngeal epithelial cells N5, NP69, and nasopharyngeal carcinoma cells CNE1. After the cell models was built,CCK-8 assay was performed to evaluate the effect of transfection on cell proliferation each day from day 1 to 5. Real-time PCR was carried out to evaluate the expression of HOTAIR. RESULTS: Successful modeling of RBM24 knocked down were confirmed by real-time PCR. The knockdown significantly induced proliferation of CNE1 (5.11±0.03) and N5 (2.09±0.18),compared with the control group (4.53±0.05 and 1.73±0.12, respectively). It also upregulated HOTAIR expression in CNE1 (67.54±1.87) and N5 (7.81±1.90), compared with the control group (1.00±0.21 and 1.00±0.19, respectively, all with P < 0.05). But it had no obvious effect on the proliferation and HOTAIR expression in NP69 (all with P > 0.05). CONCLUSION: RBM24 inhibited cell proliferation of nasopharyngeal carcinoma cell CNE1 and immortalized nasopharyngeal epithelial cells N5,through downregulation of HOTAIR expression.

Key words: RBM24, nasopharyngeal carcinoma, immortalized nasopharyngeal epithelial cells, HOTAIR, proliferation

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