X射线照射对人脐静脉内皮细胞焦亡的影响

doi:10.3969/j.issn.1004-616x.2021.02.002

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (2): 89-94.doi: 10.3969/j.issn.1004-616x.2021.02.002

X射线照射对人脐静脉内皮细胞焦亡的影响

李辰, 田梅, 齐雪松, 王春燕, 曲功霖, 邵帅, 王成芳, 苟巧

中国疾病预防控制中心辐射防护与核安全医学所, 辐射防护与核应急中国疾病预防控制中心重点实验室, 北京 100088

收稿日期:2020-11-23 修回日期:2021-03-15 出版日期:2021-03-30 发布日期:2021-04-12
通讯作者: 苟巧,E-mail:gouqiao@nirp.chinacdc.cn E-mail:gouqiao@nirp.chinacdc.cn
作者简介:李辰,E-mail:lichen@nirp.chinacdc.cn。
基金资助:
中国疾控中心辐射安全所青年科学研究所长基金（2020-07）

Induction of pyroptosis by X-rays in human umbilical vein endothelial cells

LI Chen, TIAN Mei, QI Xuesong, WANG Chunyan, QU Gonglin, SHAO Shuai, WANG Chengfang, GOU Qiao

National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, Beijing 100088, China

Received:2020-11-23 Revised:2021-03-15 Online:2021-03-30 Published:2021-04-12

摘要/Abstract

摘要： 目的：探讨X射线照射对人脐静脉内皮细胞（HUVEC）焦亡的影响。方法：单次10 Gy X射线照射HUVEC细胞，采用ELISA法检测照射后0~72 h HUVEC分泌细胞因子IL-1β、IL-6、TNF-α和IL-18的浓度，Western blot法检测照射后6~48 h HUVEC内Caspase-1的活化水平，采用透射电子显微镜检测照射后72 h HUVEC形态的变化。采用流式细胞术检测单次10 Gy以及多次小剂量（2.5 Gy/d，连续4 d）照射后HUVEC焦亡率的变化。结果：10 Gy X射线照射后0~72 h HUVEC中IL-1β、IL-6、TNF-α和IL-18浓度与对照组（0 Gy组）相比均明显升高（P<0.05）。10 Gy X射线照射后6~48 h细胞内Caspase-1活化水平升高（P<0.05），细胞呈现体积增大肿胀状态。单次10 Gy照射组和多次小剂量照射组细胞焦亡率均明显高于空白对照组（P<0.01）；此外，单次10 Gy照射组细胞焦亡率较多次小剂量照射组细胞升高约1倍（P<0.01）。结论：X射线照射可引起人脐静脉内皮细胞HUVEC焦亡；在总剂量相同情况下，单次大剂量照射引起的HUVEC焦亡水平明显高于多次小剂量照射。

关键词: X射线, 人脐静脉内皮细胞, 细胞焦亡, 半胱氨酸蛋白酶-1

Abstract: OBJECTIVE: To investigate the effects of X-ray irradiation on pyroptosis of human umbilical vein endothelial cells (HUVEC). METHODS: Pyroptosis is an inflammatory programmed cell death process. Cells were irradiated with a single dose of 10 Gy X-ray. In order to investigate the occurrence of X-ray induced pyroptosis,levels of IL-1 β,IL-6,TNF-α and IL-18 which were secreted by HUVEC cells from 0 to 72 h after the radiation were determined using the ELISA assay. Caspase-1 which is cleaved to the activated state is a key step of pyroptosis. Therefore,the activated levels of Caspase-1 were detected using Western blot at 6-48 h after the single dose of 10 Gy X-ray irradiation. Morphological changes of HUVECs were detected using transmission electron microscopy (TEM) at 72 h after the irradiation. Flow cytometry (FCM) was used to detect the changes of pyroptosis after a single dose and a small dose of multiple exposures (2.5 Gy/d×4 d). RESULTS: Compared with the control group,the levels of IL-1 β,IL-6,TNF-α and IL-18 in HUVEC cells 0-72 h after the radiation were significantly higher (P<0.05) and the levels of activated Caspase-1 at 6-48 h after the radiation was increased (P<0.05). The cell volumes were increased with swelling. The percentages of pyroptoti cells after the single and multiple exposures were significantly higher than that of the control group (P<0.01). In addition,the pyroptotic death rates after the single dose was twice as high as that after the multiple doses (P<0.01). CONCLUSION: X-ray exposure induced pyroptosis in HUVEC cells. Under the same total dose,the damage levels from the single dose exposure were significantly higher than that from the multiple exposures.

Key words: X-ray, HUVEC, pyroptosis, Caspase-1

中图分类号:

R994.6

李辰, 田梅, 齐雪松, 王春燕, 曲功霖, 邵帅, 王成芳, 苟巧. X射线照射对人脐静脉内皮细胞焦亡的影响[J]. 癌变·畸变·突变, 2021, 33(2): 89-94.

LI Chen, TIAN Mei, QI Xuesong, WANG Chunyan, QU Gonglin, SHAO Shuai, WANG Chengfang, GOU Qiao. Induction of pyroptosis by X-rays in human umbilical vein endothelial cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 89-94.

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X射线照射对人脐静脉内皮细胞焦亡的影响

Induction of pyroptosis by X-rays in human umbilical vein endothelial cells

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