长链非编码RNA与mRNA在急性髓性白血病中的异常表达及其功能初探

doi:10.3969/j.issn.1004-616x.2021.02.005

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (2): 110-115.doi: 10.3969/j.issn.1004-616x.2021.02.005

长链非编码RNA与mRNA在急性髓性白血病中的异常表达及其功能初探

田薇^1,2, 方延², 吴晗恬², 吉布强³, 夏昭林²

1. 新疆医科大学公共卫生学院, 新疆乌鲁木齐 830011;
2. 复旦大学公共卫生学院, 上海 200032;
3. 山东省临沂市人民医院血液科, 山东临沂 276100

收稿日期:2020-11-09 修回日期:2020-12-17 出版日期:2021-03-30 发布日期:2021-04-12
作者简介:田薇,E-mail:wei4148@163.com
基金资助:
新疆维吾尔自治区高校科研计划项目（XJEDU2020Y023）

Expressions and activities of lncRNAs and mRNAs in acute myeloid leukemia

TIAN Wei^1,2, FANG Yan², WU Hantian², JI Buqiang³, XIA Zhaolin²

1. Public Health College, Xinjiang Medical University, Urumqi 830011, Xinjiang;
2. Public Health College, Fudan University, Shanghai 200032;
3. Department of Hematology, Linyi People's Hospital, Linyi 276100, Shandong, China

Received:2020-11-09 Revised:2020-12-17 Online:2021-03-30 Published:2021-04-12

摘要/Abstract

摘要： 目的：探讨急性髓性白血病患者和健康对照人群中长链非编码RNA （lncRNA）和mRNA的差异表达，初步建立急性髓性白血病患者lncRNA异常表达谱。方法：采用Human lncRNA芯片，对急性髓性白血病患者及健康对照人群外周静脉血各6例共12个标本进行lncRNAs及mRNAs检测，并采用基因本体（GO）分析和KEGG通路初步分析差异表达基因的生物学功能。结果：与健康对照组相比，急性髓性白血病组患者差异表达的lncRNA有3 256个，其中1 161个表达上调，2 095个表达下调；差异表达的mRNA有3 544个，其中1 950个表达上调，1 594个表达下调；GO分析表明，差异表达基因功能注释主要包括化学刺激感受、有丝分裂细胞周期的G1/S转变、炎症反应等。KEGG分析表明，差异表达基因主要富集在移植物抗宿主反应疾病、细胞循环及人类嗜T淋巴细胞病毒I型感染等通路上。结论：本研究筛选出急性髓性白血病患者异常表达的部分lncRNA和mRNA，为深入研究白血病发病分子机制提供了理论依据。

关键词: 急性髓性白血病, 长链非编码RNA, mRNA, 基因芯片

Abstract: OBJECTIVE: To explore the differential expression and activities of lncRNAs and mRNAs in lymphocytes from acute myeloid leukemia patients compared with that from normal controls. METHODS: Microarrays were used to detect discrepant lncRNAs and mRNAs in peripheral lymphocytes from 6 acute myeloid leukemia patients compared with 6 normal controls. GO and KEGG were used to analyze the primary biological functions of differentially expressed genes. RESULTS: In lymphocytes from the two groups of individuals,there were 3 256 differentially expressed lncRNAs between patients and controls. Among these lncRNAs,1 161 lncRNAs were upregulated and 2 059 were downregulated. In addition,there were 3 544 differentially expressed mRNAs between patients and controls;1 950 mRNA were upregulated and 1 594 were downregulated. GO analyses show that functional annotation of the differential expression genes mainly included detection of chemical stimulus involved in sensory perception of smell,G1/S transition of mitotic cell cycle and inflammatory response. The graft versus host disease and cell cycle,HTLV-I infection and other pathways were shown by KEGG. CONCLUSION: These findings extended the current knowledge and provided some novel mechanisms on pathogenesis of leukemia.

Key words: acute myeloid leukemia, lncRNA, mRNA, gene chip

中图分类号:

R733.7

田薇, 方延, 吴晗恬, 吉布强, 夏昭林. 长链非编码RNA与mRNA在急性髓性白血病中的异常表达及其功能初探[J]. 癌变·畸变·突变, 2021, 33(2): 110-115.

TIAN Wei, FANG Yan, WU Hantian, JI Buqiang, XIA Zhaolin. Expressions and activities of lncRNAs and mRNAs in acute myeloid leukemia[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 110-115.

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长链非编码RNA与mRNA在急性髓性白血病中的异常表达及其功能初探

Expressions and activities of lncRNAs and mRNAs in acute myeloid leukemia

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