CXCR-2在食管鳞癌组织中的表达及其与MMP-9、VEGF的相关性研究

doi:10.3969/j.issn.1004-616x.2021.03.012

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (3): 225-229,240.doi: 10.3969/j.issn.1004-616x.2021.03.012

CXCR-2在食管鳞癌组织中的表达及其与MMP-9、VEGF的相关性研究

范玉宏¹, 周海丰², 范爽², 王立坤³, 侯雅雄¹, 刘博⁴

1. 河北北方学院附属第一医院病理教研室, 河北张家口 075000;
2. 河北北方学院附属第一医院普通外科, 河北张家口 075000;
3. 河北北方学院附属第一医院超声医学科, 河北张家口 075000;
4. 河北北方学院附属第一医院病理科, 河北张家口 075000

收稿日期:2021-01-15 修回日期:2021-04-21 出版日期:2021-05-30 发布日期:2021-06-09
通讯作者: 侯雅雄，E-mail：wuxl@hebeinu.edu.cn E-mail:wuxl@hebeinu.edu.cn
作者简介:范玉宏，E-mail：fyh-82821@163.com。
基金资助:
河北省卫生计划委员会2017医学重点课题计划（20170791）；张家口市科技局2020指导性计划（2021084D）

Expression of CXCR-2 and its effects on MMP-9 and VEGF in esophageal squamous carcinoma cells

FAN Yuhong¹, ZHOU Haifeng², FAN Shuang², WANG Likun³, HOU Yaxiong¹, LIU Bo⁴

1. Pathological Staff Room, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000;
2. Department of General Surgery, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000;
3. Department of Ultrasound, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000;
4. Department of Pathology, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei, China

Received:2021-01-15 Revised:2021-04-21 Online:2021-05-30 Published:2021-06-09

摘要/Abstract

摘要： 目的：探讨CXC趋化因子受体2（CXCR-2）在食管鳞癌中的表达及其与基质金属蛋白酶9（MMP-9）、血管内皮生长因子（VEGF）表达的相关性。方法：收集河北北方学院附属第一医院胸外科2016年12月—2019年12月诊治的70例食管鳞癌患者食管癌组织及癌旁正常组织，分别采用实时荧光定量PCR法（qPCR）和免疫组化法检测CXCR-2、MMP-9、VEGF mRNA和蛋白在两组标本中的表达情况，采用Pearson相关分析法分析CXCR-2、MMP-9和VEGF表达的相关性，受试者操作特征（ROC）曲线分析CXCR-2的表达对食管鳞癌的诊断意义。结果：qPCR检测结果显示，癌组织中CXCR-2、MMP-9、VEGF mRNA的相对表达水平高于癌旁正常组织（均为P < 0.01）；免疫组化检测结果显示，癌组织中CXCR-2、MMP-9、VEGF蛋白的阳性表达率高于癌旁正常组织（均为P < 0.01）；且癌组织中CXCR-2的表达与MMP-9、VEGF的表达呈正相关（r分别为0.488和0.491，均为P < 0.01）；癌组织中CXCR-2表达的ROC曲线下面积为0.938[95% CI （0.908，0.972），P < 0.05]，最佳截断点为0.77。其诊断的灵敏度和特异度分别为91.2%和80.9%。结论：食管鳞癌组织中CXCR-2、MMP-9和VEGF呈高水平表达，且与TNM分期、淋巴结转移和远处转移关系密切，CXCR-2表达水平的检测对食管鳞癌诊断有重要价值。

关键词: 食管鳞癌, CXC趋化因子受体2, 基质金属蛋白酶9, 血管内皮生长因子, 受试者操作特征曲线

Abstract: OBJECTIVE： To investigate expression levels of CXC chemokine receptor 2(CXCR-2) and its effect on matrix metalloproteinase- 9 (MMP- 9)， and vascular endothelial growth factor (VEGF) in esophageal squamous carcinoma cells. METHODS： From December 2016 to December 2019， 70 cases of esophageal squamous cell carcinoma tissues and adjacent normal tissues were collected from the Department of thoracic surgery，the First Affiliated Hospital of Hebei North University. Expression levels of CXCR-2，MMP-9，VEGF mRNAs and proteins were detected by real-time fluorescence quantitative PCR (qPCR) and immunohistochemistry. Pearson correlation analysis was used to analyze the correlation of CXCR- 2，MMP-9 and VEGF expression. Receiver operating characteristic (ROC) curves were used to analyze the diagnostic significance of CXCR- 2 expression in esophageal squamous cell carcinoma. RESULTS： qPCR results show that the expression levels of CXCR-2 mRNA，MMP-9 mRNA，and VEGF mRNA were significantly higher in the esophageal squamous cell carcinoma tissues compared with their adjacent normal tissues(all P < 0.01). Similarly，the protein expressions of CXCR-2，MMP-9，and VEGF were significantly higher in the carcinoma tissues than in their adjacent normal tissues (all P < 0.01). Moreover，CXCR-2 expressions were significantly and positively correlated with expression levels of MMP- 9 and VEGF in the carcinoma tissues (r=0.488 and 0.491，P < 0.01). Results from the ROC curve analyses show that the expression levels of CXCR- 2 had auxiliary diagnostic value for esophageal squamous cell carcinoma. The area under the ROC curve was 0.938 [95% CI (0.908，0.972)]，and best cut-off values for gastric cancer was 0.77， the sensitivity was 91.2% and specificity was 80.9%. CONCLUSION： CXCR-2，MMP-9 and VEGF were highly expressed in the squamous cell carcinoma，and were closely related to TNM stage，lymph node metastasis and distant metastasis. Therefore，expression levels of CXCR-2 can be of great value in the diagnosis of esophageal squamous cell carcinoma.

Key words: esophageal squamous cell cancer, CXCR-2, MMP-9, VEGF, ROC curve

中图分类号:

R979.1

范玉宏, 周海丰, 范爽, 王立坤, 侯雅雄, 刘博. CXCR-2在食管鳞癌组织中的表达及其与MMP-9、VEGF的相关性研究[J]. 癌变·畸变·突变, 2021, 33(3): 225-229,240.

FAN Yuhong, ZHOU Haifeng, FAN Shuang, WANG Likun, HOU Yaxiong, LIU Bo. Expression of CXCR-2 and its effects on MMP-9 and VEGF in esophageal squamous carcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 225-229,240.

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CXCR-2在食管鳞癌组织中的表达及其与MMP-9、VEGF的相关性研究

Expression of CXCR-2 and its effects on MMP-9 and VEGF in esophageal squamous carcinoma cells

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