食管鳞癌中SERPINE1 mRNA的表达及其临床意义

doi:10.3969/j.issn.1004-616x.2021.04.001

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (4): 245-249.doi: 10.3969/j.issn.1004-616x.2021.04.001

• 论著 • 下一篇

食管鳞癌中SERPINE1 mRNA的表达及其临床意义

常晨, 杨荔艳, 蔡洪庆, 苑青, 郝佳洁, 王明荣

国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院, 分子肿瘤学国家重点实验室, 北京 100021

收稿日期:2021-05-08 修回日期:2021-06-02 出版日期:2021-07-30 发布日期:2021-07-29
通讯作者: 郝佳洁,E-mail:hjj8173@126.com;王明荣,E-mail:wangmr2015@126.com E-mail:hjj8173@126.com;wangmr2015@126.com
作者简介:常晨,E-mail:changchen9185@163.com。
基金资助:
国家重点研发计划项目（2017YFE0112100）；国家自然科学基金（81930077）；中国医学科学院医学与健康科技创新工程（2019-I2M-1-003）

Expressions and clinical implications of SERPINE1 mRNA in esophageal squamous carcinoma tissues

CHANG Chen, YANG Liyan, CAI Hongqing, YUAN Qing, HAO Jiajie, WANG Mingrong

State Key Laboratory of Molecular Oncology, Center for Cancer Precision Medicine, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China

Received:2021-05-08 Revised:2021-06-02 Online:2021-07-30 Published:2021-07-29

摘要/Abstract

摘要： 目的：检测SERPINE1 mRNA在食管鳞癌中的表达并评价其临床意义。方法：采用组织微阵列和RNAscope原位杂交技术检测236例食管鳞癌组织及其配对手术切缘正常组织中SERPINE1 mRNA的表达水平，分析其表达与临床病理指标及预后的相关性。结果：在23.7%（56/236）的食管鳞癌组织中有SERPINE1 mRNA的高表达，而在癌旁正常组织中无表达。SERPINE1 mRNA表达水平与性别、肿瘤分级、浸润深度及临床分期均显著正相关（r分别为7.939、6.618、10.939、6.671，均为P<0.05）。Kaplan-Meier生存曲线显示SERPINE1 mRNA高表达患者的生存时间显著短于低表达患者（P<0.05）。多因素Cox回归分析表明，SERPINE1 mRNA高表达是食管鳞癌患者不良预后的独立预测因素。结论：SERPINE1 mRNA在食管鳞癌组织中表达升高，其高表达与食管鳞癌患者预后不良相关，有可能作为食管鳞癌预后评估的分子标志。

关键词: 食管鳞癌, RNA原位杂交, SERPINE1, 分子标志

Abstract: OBJECTIVE: To investigate expression status and clinical relevance of SERPINE1 mRNA in esophageal squamous cell carcinoma (ESCC) tissues. METHODS: Expressions of SERPINE1 mRNA were determined using tissue microarrays and RNAscope in situ hybridizations in surgically-resected tumor tissues and adjacent normal epithelia from 236 ESCC patients. Relationships between the mRNA expression and clinicopathological characteristics as well as prognosis were analyzed. RESULTS: Increased expression of SERPINE1 mRNA was observed in 23.7% (56/236) of the tumor tissues. However,there was no expression of SERPINE1 mRNA in normal tissues which were adjacent to the carcinomas. The increased expressions were positively correlated with genders,histological grades,T stages and clinical stages (r=7.939,6.618,10.939,6.671,respectively;each P<0.05). Kaplan-Meier survival curves show that patients with high expressions of SERPINE1 mRNA had shorter survival times than those with low expressions (P<0.05). Cox multiple regression analyses indicate that SERPINE1 mRNA expression was an independent prognosis factor in ESCC. CONCLUSION: SERPINE1 mRNA was expressed highly in ESCC tissues. Our data indicate that the expression may serve as a molecular biomarker for predicting poor prognosis of ESCC patients.

Key words: esophageal squamous cell carcinoma, RNA in situ hybridization, SERPINE1, biomarker

中图分类号:

R735.1

常晨, 杨荔艳, 蔡洪庆, 苑青, 郝佳洁, 王明荣. 食管鳞癌中SERPINE1 mRNA的表达及其临床意义[J]. 癌变·畸变·突变, 2021, 33(4): 245-249.

CHANG Chen, YANG Liyan, CAI Hongqing, YUAN Qing, HAO Jiajie, WANG Mingrong. Expressions and clinical implications of SERPINE1 mRNA in esophageal squamous carcinoma tissues[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 245-249.

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食管鳞癌中SERPINE1 mRNA的表达及其临床意义

Expressions and clinical implications of SERPINE1 mRNA in esophageal squamous carcinoma tissues

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