烟气暴露30天对大鼠心脏基因表达谱的影响

doi:10.3969/j.issn.1004-616x.2021.04.009

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (4): 291-295.doi: 10.3969/j.issn.1004-616x.2021.04.009

烟气暴露30天对大鼠心脏基因表达谱的影响

李梅¹, 苏加坤², 尹晶晶¹, 徐达², 秦秀军¹, 安全¹, 蔡继宝²

1. 中国辐射防护研究院药物毒理与放射损伤药物山西省重点实验室, 山西太原 030006;
2. 江西中烟工业有限责任公司, 江西南昌 330096

收稿日期:2020-11-09 修回日期:2020-12-21 出版日期:2021-07-30 发布日期:2021-07-29
通讯作者: 蔡继宝,E-mail:jbcai@ustc.edu.cn E-mail:jbcai@ustc.edu.cn
作者简介:李梅,E-mail:1021315677@qq.com。
基金资助:
中国烟草总公司重大专项项目（110201401025）

Effects of 30-day smoke exposure on gene expression profile in hearts of rats

LI Mei¹, SU Jiakun², YIN Jingjing¹, XU Da², QIN Xiujun¹, AN Quan¹, CAI Jibao²

1. Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, China Institute for Radiation Protection, Taiyuan 030006, Shanxi;
2. Tobacco Jiangxi Industrial Co., Ltd., Nanchang 330096, Jiangxi, China

Received:2020-11-09 Revised:2020-12-21 Online:2021-07-30 Published:2021-07-29

摘要/Abstract

摘要： 目的：利用基因芯片技术研究烟气暴露30 d对大鼠心脏基因表达的影响。方法：6只雄性SD大鼠分为对照组和烟气暴露组。烟气暴露组经主流烟气暴露，对照组不进行干预，30 d后取大鼠心脏，应用基因芯片技术进行差异表达基因筛选，利用基因本体（GO）和京都基因与基因组百科全书（KEGG）数据库对差异基因进行生物信息学分析。结果：与对照组比较，烟气暴露组筛选出差异表达2倍以上的基因86个，其中上调表达60个，下调表达26个。差异表达基因富集于292种生物过程，72种细胞成分和96类分子功能以及61个信号通路。主要涉及促分裂原活化蛋白激酶结合、α-βT细胞受体复合物、细胞外渗的正调节、络氨酸代谢等相关基因通路。结论：烟气暴露30 d所致大鼠心脏的异常表达基因与信号通路主要涉及分子结合、细胞周期、信号转导等多个方面。

关键词: 烟气暴露, 大鼠心脏, 基因, 生物信息学

Abstract: OBJECTIVE: To study effects of smoke exposure on gene expression in hearts of rats using gene chip technology. METHODS: Six male SD rats were divided into two groups:control (n=3) and smoke exposure (n=3). The exposure group were exposed to mainstream smoke for 30 days. Rat hearts were removed and differentially expressed genes were screened using gene chip technology. Differentially-expressed genes were analyzed using gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) databases. RESULTS: Compared with the control group,86 genes with differential expression of more than 2 times were screened out in the smoke exposure group,among which 60 genes were up-regulated and 26 genes were down-regulated. Differentially expressed genes were enriched in 292 biological processes:72 cellular components,96 molecular functions and 61 signaling pathways. These processes were involved in mitogen-activated protein kinase binding,alpha-beta T cell receptor complex,positive regulation of cellular extravasation,and tyrosine metabolism. CONCLUSION: After exposure to smoke for 30 days,differentially expressed genes and signaling pathways in the hearts of rats were mainly involved in molecular binding,cell cycle control and signal transduction,etc.

Key words: smoke exposure, rat heart, gene, bioinformatics

中图分类号:

R994.6

李梅, 苏加坤, 尹晶晶, 徐达, 秦秀军, 安全, 蔡继宝. 烟气暴露30天对大鼠心脏基因表达谱的影响[J]. 癌变·畸变·突变, 2021, 33(4): 291-295.

LI Mei, SU Jiakun, YIN Jingjing, XU Da, QIN Xiujun, AN Quan, CAI Jibao. Effects of 30-day smoke exposure on gene expression profile in hearts of rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 291-295.

参考文献

[1] 陈伟伟, 高润霖, 刘力生, 等. 《中国心血管病报告2016》概要[J]. 中国循环杂志, 2017, 32(6):521-530.
[2] 施惠华. 高血压发病危险因素的性别差异研究[J]. 现代检验医学杂志, 2013, 28(3):95-98.
[3] 余振球, 牟建军, 朱鼎良. 社区高血压防治[M]. 北京:科学出版社, 2010:186-193.
[4] 林荣, 骆秉铨, 吴良金, 等. 多普勒超声心动图检测吸烟对血管内皮依赖性舒张功能的影响[J]. 临床荟萃, 2000, 15(20):918-919.
[5] 唐雪梅, 顾鹏, 张军辉. 超声心动图评价长期吸烟对心脏形态学和功能学的影响[J]. 中华临床医师杂志:电子版, 2012, 6(18):5683-5685.
[6] HOLMSTRÖM K M, FINKEL T. Cellular mechanisms and physiological consequences of redox-dependent signalling[J]. Nat Rev Mol Cell Biol, 2014, 15(6):411-421.
[7] TALUKDER M A, JOHNSON W M, VARADHARAJ S, et al. Chronic cigarette smoking causes hypertension, increased oxidative stress, impaired NO bioavailability, endothelial dysfunction, and cardiac remodeling in mice[J]. Am J Physiol Heart Circ Physiol, 2011, 300(1):H388-H396.
[8] GIACOMIN E, PALMERINI E, BALLO P, et al. Acute effects of caffeine and cigarette smoking on ventricular long-axis function in healthy subjects[J]. Cardiovasc Ultrasound, 2008, 6:9.
[9] CASTARDELI E, PAIVA S A, MATSUBARA B B, et al. Chronic cigarette smoke exposure results in cardiac remodeling and impaired ventricular function in rats[J]. Arq Bras Cardiol, 2005, 84(4):320-324.
[10] GU L, PANDEY V, GEENEN D L, et al. Cigarette smoke-induced left ventricular remodeling is associated with activation of mitogen-activated protein kinases[J]. Eur J Heart Fail, 2008, 10(11):1057-1064.
[11] COHN J N, FERRARI R, SHARPE N. Cardiac remodeling:concepts and clinical implications:a consensus paper from an international forum on cardiac remodeling. Behalf of an International Forum on Cardiac Remodeling[J]. J Am Coll Cardiol, 2000, 35(3):569-582.
[12] 魏秋华, 苏瑞斌. 细胞色素P-450:心脏与冠脉循环中的新靶标[J]. 国外医学:药学分册, 2006, 33(2):104-106.
[13] LüSCHER T F. Heart failure and left ventricular remodelling in HFrEF and HFpEF[J]. Eur Heart J, 2016, 37(5):423-424.
[14] 勾白冰, 黄晶, 钱俊, 等. 经导管肾交感神经消融术治疗慢性心力衰竭的有效性和安全性:Meta分析[J]. 中国介入影像与治疗学, 2019, 16(6):342-348.

[1]	国瑞贤, 谢广云, 韩莹. 基于转录组测序方法研究加替沙星对小鼠的肝损伤作用[J]. 癌变·畸变·突变, 2022, 34(1): 20-24.
[2]	陈敏娜, 李春兰, 刘静. 转录因子STAT5A启动子荧光素酶报告基因质粒的构建及鉴定[J]. 癌变·畸变·突变, 2022, 34(1): 53-56,71.
[3]	王苗, 王全凯, 李昕苇, 马顺鹏, 乌瀚宝栎尔, 许建宁. 甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化相关m⁶A修饰异常mRNA的分析[J]. 癌变·畸变·突变, 2021, 33(6): 405-409.
[4]	杨婵君, 陈雯恬, 刘静. 胶原蛋白作为胃癌潜在生物标志物的生物信息学分析[J]. 癌变·畸变·突变, 2021, 33(6): 410-419.
[5]	杨兴肖, 张雪原, 邹乃祎, 单保恩, 马鸣, 祝淑钗. 干扰HMGB1基因表达对食管鳞癌细胞侵袭迁移及放射敏感性的影响[J]. 癌变·畸变·突变, 2021, 33(5): 327-333.
[6]	陆雪梅, 许铭炎, 刘庭英, 张秋芳, 邓小玲. 潮汕地区汉族人群MTHFR基因rs1801131多态性与非综合征型唇腭裂的相关性研究[J]. 癌变·畸变·突变, 2021, 33(5): 334-337,344.
[7]	周殿明, 张大龙, 张静, 何宁, 高峥, 张倩, 周晓丽, 钱智勇. 抗虫耐除草剂玉米对大鼠的亚慢性毒性研究[J]. 癌变·畸变·突变, 2021, 33(5): 383-387,392.
[8]	李蕊瑞, 张立实, 陈锦瑶. 人群PIG-A基因突变测定的研究进展[J]. 癌变·畸变·突变, 2021, 33(5): 396-400.
[9]	郑建涛, 杨勇, 周东. 基于TCGA数据分析CX43表达在胶质瘤中的临床意义及其与免疫细胞浸润的关系[J]. 癌变·畸变·突变, 2021, 33(4): 250-254,261.
[10]	曾卓颖, 吴德生, 鲁晶晶, 廖慧, 赖洪飘, 袁建辉, 胡章立. 雌激素受体α介导氧化磷酸化通路在肺癌发生中的作用[J]. 癌变·畸变·突变, 2021, 33(4): 262-268.
[11]	徐启璐, 高慧, 冉学红, 王军军, 张俊英, 刘丽萍. 两种异常核型髓系肿瘤的基因突变及临床相关性分析[J]. 癌变·畸变·突变, 2021, 33(4): 296-301,306.
[12]	张娜, 范志露, 杨荔艳, 常晨, 蔡岩, 郝佳洁, 王明荣. 食管鳞癌中SERPINB5表达降低的作用机制及其临床意义[J]. 癌变·畸变·突变, 2021, 33(2): 101-109.
[13]	田薇, 方延, 吴晗恬, 吉布强, 夏昭林. 长链非编码RNA与mRNA在急性髓性白血病中的异常表达及其功能初探[J]. 癌变·畸变·突变, 2021, 33(2): 110-115.
[14]	李柏茹, 秦双建, 李闰冰, 蔡颖, 郑凯, 曾明, 肖芳, 徐新云. p38MAPK基因对PM_2.5染毒HK-2细胞部分癌基因和凋亡相关基因表达的影响[J]. 癌变·畸变·突变, 2021, 33(2): 129-135,142.
[15]	李柏茹, 秦双建, 李闰冰, 蔡颖, 郑凯, 王冰玉, 曾明, 肖芳, 徐新云. PM_2.5对HK-2肾细胞部分癌基因和凋亡基因表达的影响[J]. 癌变·畸变·突变, 2021, 33(1): 6-11.

烟气暴露30天对大鼠心脏基因表达谱的影响

Effects of 30-day smoke exposure on gene expression profile in hearts of rats

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价