PM2.5混悬液对3种癌细胞侵袭和迁移能力的影响

doi:10.3969/j.issn.1004-616x.2022.02.009

癌变·畸变·突变 ›› 2022, Vol. 34 ›› Issue (2): 124-128,133.doi: 10.3969/j.issn.1004-616x.2022.02.009

• 论著 • 上一篇

PM_2.5混悬液对3种癌细胞侵袭和迁移能力的影响

李柏茹^1,3, 秦双建^1,3, 蔡颖^2,3, 李闰冰^2,3, 关岚¹, 肖芳¹, 曾明¹, 徐新云³

1. 中南大学湘雅公共卫生学院, 湖南长沙 410078;
2. 南华大学公共卫生学院, 湖南衡阳 421001;
3. 深圳市疾病预防控制中心, 广东深圳 518055

收稿日期:2021-10-23 修回日期:2022-02-27 发布日期:2022-04-07
通讯作者: 徐新云,E-mail:xyxu2008@163.com E-mail:xyxu2008@163.com
作者简介:李柏茹,E-mail:15733295728@163.com;秦双建,E-mail:1375195449@qq.com;蔡颖,E-mail:1479288556@qq.com。
基金资助:
深圳市科技研发项目(JCYJ20190807102205480)

Effects of PM_2.5 on invasion and migration abilities in three types of cancer cells

LI Boru^1,3, QIN Shuangjian^1,3, CAI Ying^2,3, LI Runbing^2,3, GUAN Lan¹, XIAO Fang¹, ZENG Ming¹, XU Xinyun³

1. Xiangya School of Public Health, Central South University, Changsha 410078, Hunan;
2. School of Public Health, University of South China, Hengyang 421001, Hunan;
3. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China

Received:2021-10-23 Revised:2022-02-27 Published:2022-04-07

摘要/Abstract

摘要： 目的：探讨PM_2.5混悬液对人肺癌A549细胞、人肝癌HepG2细胞和人肾癌A498细胞迁移和侵袭能力的影响。方法：采用10、50μg/mL的PM_2.5混悬液分别对A549、HepG2和A498细胞染毒24h，Transwell小室实验检测细胞迁移、侵袭能力。结果：迁移实验结果显示，阴性对照组、10和50μg/mL PM_2.5染毒组A549细胞穿膜细胞数分别为(356.33±30.92)、(476.33±28.50)和(563.33±74.00)个；HepG2细胞分别为(86.42±4.75)、(140.00±16.15)和(234.17±9.06)个；A498细胞分别为(42.33±6.43)、(80.00±6.08)和(174.67±25.01)个。侵袭实验结果显示，阴性对照组、10和50μg/mL PM_2.5染毒组A549细胞的穿膜细胞数分别为(188.33±9.45)、(313.00±7.94)和(404.00±57.66)个；HepG2细胞分别为(48.33±3.26)、(97.75±6.63)和(123.92±8.57)个；A498细胞分别为(5.67±5.03)、(21.33±5.13)和(40.67±6.81)个。在迁移实验和侵袭实验中，与阴性对照组相比，PM_2.5染毒组中的3种细胞穿膜细胞数均增加(P<0.05或0.01)。结论：PM_2.5可增强A549、HepG2和A498细胞的迁移和侵袭能力。

关键词: 大气细颗粒物, A549细胞, HepG2细胞, A498细胞, 侵袭, 迁移

Abstract: OBJECTIVE：To investigate effects of PM_2.5 on the migration and invasion abilities in three types of cancer cells： human lung (A549)， human liver (HepG2) and human renal (A498) cancer cells. METHODS：The A549，HepG2 and A498 cells were exposed to PM_2.5 suspension at 10 and 50 μg/mL for 24 h. The transwell assay was performed to detect cell migration and invasion abilities. RESULTS： The results from the migration assay show that the number of transmembrane A549 cells in the negative control， 10 and 50 μg/mL PM_2.5 exposure groups were (356.33±30.92)，(476.33±28.50) and (563.33±74.00)，respectively. Data for the HepG2 cells were (86.42±4.75)，(140.00±16.15) and (234.17±9.06); and those for the A498 cells were (42.33±6.43)，(80.00±6.08) and (174.67±25.01)，respectively. The results from the invasion assay show that the number of transmembrane A549 cells in the three experimental groups were (188.33±9.45)，(313.00±7.94) and (404.00±57.66)，respectively. Data for the HepG2 cells were (48.33±3.26)，(97.75±6.63) and (123.92±8.57)；and those for the A498 cells were (5.67 ±5.03)，(21.33 ±5.13) and (40.67 ±6.81)，respectively. In the migration and invasion experiments，compared with the negative control group，the numbers of penetrating cells of the three types of cells in the PM_2.5 exposure group were significantly increased (P<0.05). CONCLUSION： PM_2.5 significantly enhanced the abilities of migration and invasion in A549，HepG2 and A498 cells.

Key words: PM_2.5, A549 cells, HepG2 cells, A498 cells, invasion, migration

中图分类号:

R122.7

李柏茹, 秦双建, 蔡颖, 李闰冰, 关岚, 肖芳, 曾明, 徐新云. PM_2.5混悬液对3种癌细胞侵袭和迁移能力的影响[J]. 癌变·畸变·突变, 2022, 34(2): 124-128,133.

LI Boru, QIN Shuangjian, CAI Ying, LI Runbing, GUAN Lan, XIAO Fang, ZENG Ming, XU Xinyun. Effects of PM_2.5 on invasion and migration abilities in three types of cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(2): 124-128,133.

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PM_2.5混悬液对3种癌细胞侵袭和迁移能力的影响

Effects of PM_2.5 on invasion and migration abilities in three types of cancer cells

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PM2.5混悬液对3种癌细胞侵袭和迁移能力的影响

Effects of PM2.5 on invasion and migration abilities in three types of cancer cells

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PM_2.5混悬液对3种癌细胞侵袭和迁移能力的影响

Effects of PM_2.5 on invasion and migration abilities in three types of cancer cells