苯并[b]荧蒽和二苯并[a,h]蒽对成年雄性大鼠氧化应激和炎症因子的影响

doi:10.3969/j.issn.1004-616x.2024.01.003

癌变·畸变·突变 ›› 2024, Vol. 36 ›› Issue (1): 16-20.doi: 10.3969/j.issn.1004-616x.2024.01.003

苯并[b]荧蒽和二苯并[a,h]蒽对成年雄性大鼠氧化应激和炎症因子的影响

康真, 于天一, 刘晓波, 洪千淇, 蔡书锐, 闫海

哈尔滨市疾病预防控制中心, 黑龙江哈尔滨 150010

收稿日期:2023-09-12 修回日期:2023-12-09 出版日期:2024-02-19 发布日期:2024-02-19
通讯作者: 闫海
作者简介:康真,E-mail:kz_ivy@126.com。
基金资助:
黑龙江省卫生健康委科研课题（2020-052，20231212010393）

Effects of benzo[b]fluoranthene and dibenzo[a, h]anthracene on oxidative stress and flammatory factors in rats

KANG Zhen, YU Tianyi, LIU Xiaobo, HONG Qianqi, CAI Shurui, YAN Hai

Harbin Center for Disease Control and Prevention, Harbin 150010, Heilongjiang, China

Received:2023-09-12 Revised:2023-12-09 Online:2024-02-19 Published:2024-02-19

摘要/Abstract

摘要： 目的：探讨气管滴注苯并[b]荧蒽（BbFA）和二苯并[a，h]蒽（DahA）对成年雄性大鼠氧化应激和炎症因子的影响。方法：选取雄性SD大鼠48只，随机分成6组，分别为对照组（0 mg/kg），Bb FA低、高剂量组（分别为3×10^-4、9×10^-4mg/kg），Dah A低、高剂量组（分别为7×10^-5、2×10^-4mg/kg），Bb FA和Dah A联合组（3×10^-4mg/kg Bb FA+7×10^-5mg/kg Dah A），每组8只，每周气管滴注两次，连续染毒4周，观察不同剂量染毒组对大鼠肺灌洗液、肺组织与血清中氧化应激指标和炎症因子的影响。结果：与对照组相比，Dah A染毒组、Bb FA和Dah A联合组大鼠肺组织中SOD活力显著降低（P<0.05），且联合组中MDA、NO、i-NOS含量显著升高（P<0.05），其中MDA、i-NOS含量显著高于单独Bb FA或Dah A染毒组（P<0.05）。染毒组大鼠血清中氧化应激指标MDA含量均升高，且联合组MDA含量显著高于单独Bb FA或Dah A染毒的低剂量组（P<0.05）。与对照组比较，染毒组大鼠肺灌洗液和血清中炎症因子含量均升高，且联合组中IL-6含量显著高于单独Bb FA或Dah A染毒组（P<0.05）。结论：大鼠单独或是联合染毒Bb FA和Dah A后，可能是通过抑制SOD和CAT活性，升高MDA、NO和i-NOS活性引起体内氧化应激的改变，进而引起炎症因子IL-6水平的升高。

关键词: 苯并[b]荧蒽, 二苯并[a, h]蒽, 氧化应激, 炎症因子

Abstract: OBJECTIVE: To investigate effects of benzo[b]fluoranthene (BbFA) and dibenzo[a, h]anthracene(Dah A) exposure on oxidative stress and inflammatory factors in rats.METHODS: After 1 week of acclimatization, 48 adult male SD rats were randomly divided into exposed and control groups.The rats were exposed to Bb FA and Dah A by intratracheal instillation twice a week in 4 weeks.Inflammatory factors in BALF and serum, and oxidative stress factors in lung tissue and serum were determined.RESULTS: Serum activity of SOD was significantly decreased in the Dah A-exposed groups (P<0.05).Meanwhile, the Bb FA+Dah A exposed groups showed significantly increased levels of MDA, NO, i-NOS in the lung tissue compared with the control group (P<0.05), and the levels of MDA and i-NOS in the Bb FA+Dah A combined exposure group were significantly higher than that of the Bb FA-exposed and the Dah A-exposed groups (P<0.05).Additionaly, the content of MDA in the Bb FA+Dah A combined exposure group was significantly higher than low dose group of Bb FA-exposed group and Dah A-exposed group (P<0.05).The levels of IL-6 in the Bb FA+Dah A combined exposure group were significantly up-regulated compared with the Bb FA-exposed and the Dah A-exposed groups(P<0.05).CONCLUSION: Our results demonstrated that Bb FA and Dah A caused changes in inflammatory and oxidative factors that were related to inhibition of SOD activity and elevation of i NOS activity, and MDA, NO and IL-6 levels.

Key words: BbFA, DahA, oxidant stress, inflammatory factors

中图分类号:

R994.6

康真, 于天一, 刘晓波, 洪千淇, 蔡书锐, 闫海. 苯并[b]荧蒽和二苯并[a,h]蒽对成年雄性大鼠氧化应激和炎症因子的影响[J]. 癌变·畸变·突变, 2024, 36(1): 16-20.

KANG Zhen, YU Tianyi, LIU Xiaobo, HONG Qianqi, CAI Shurui, YAN Hai. Effects of benzo[b]fluoranthene and dibenzo[a, h]anthracene on oxidative stress and flammatory factors in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(1): 16-20.

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苯并[b]荧蒽和二苯并[a,h]蒽对成年雄性大鼠氧化应激和炎症因子的影响

Effects of benzo[b]fluoranthene and dibenzo[a, h]anthracene on oxidative stress and flammatory factors in rats

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