›› 2012, Vol. 24 ›› Issue (2): 108-110.doi: 10.3969/j.issn.1004-616x.2012.02.006

• 论著 • 上一篇    下一篇

五味子提取物预防辐射所致免疫损伤的实验研究

刘丽华，刘登湘，马 鸣，张 璁，段玉青，赵连梅，单保恩   

  1. 1. 河北医科大学第四医院 临床生物细胞室，河北 石家庄 050011；2. 河北医科大学第四医院 科研中心，河北 石家庄 050011；3. 邢台市人民医院放疗科，河北 邢台 054000
  • 收稿日期:2011-04-02 修回日期:2011-10-02 出版日期:2012-03-30 发布日期:2012-03-30
  • 通讯作者: 单保恩

Schisandra Chinensis extract on immune injury by radiation

LIU Li-hua，LIU Deng-xiang，MA Ming，ZHANG Cong，DUAN Yu-qing，ZHAO Lian-mei，SHAN Bao-en   

  1. 1. Department of Clinical Bio-cell,the Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011; 2. Research Cencer, the Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011; 3. Department of Radiotherapy，People’s Hospital of Xingtai, Xingtai 054000, Hebei, China
  • Received:2011-04-02 Revised:2011-10-02 Online:2012-03-30 Published:2012-03-30
  • Contact: SHAN Bao-en

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摘要： 目的： 研究中药五味子 (Schisandra Chinensis，SC) 提取物对辐射所致小鼠免疫功能的影响，探讨SC对辐射所致免疫功能损伤的保护作用。方法：将48只BALB/c小鼠随机分为4组，分别为SC预处理组 (SC灌胃后照射)、生理盐水组 (NS灌胃后照射）、SC对照组 (只给SC，不照射)和正常对照组 (只给NS，不照射)。6 Gy 60Co照射后24 h进行外周血白细胞和淋巴细胞计数；流式细胞仪检测小鼠外周血中CD4+、CD8+ T细胞的百分比，用免疫浊度法检测小鼠血清IgG及补体C3的含量。结果：与正常对照组相比，NS组照射24 h后外周血白细胞总数[ (3.73±1.05)×109]和淋巴细胞数量[(2.06±0.57)×109]均明显下降(P＜0.01)，而SC预处理组白细胞总数[(6.43±0.76)×109]和淋巴细胞数量[(4.15±0.95)×109]较NS均明显升高 (P＜0.01)；照射后小鼠外周血中CD3+、CD4+和CD8+ T 细胞百分比、IgG及补体C3水平较正常对照组明显降低 (P＜0.01)；SC预处理组较NS组小鼠外周血中CD4+和CD8+ T细胞绝对值、IgG及补体C3水平均明显升高 (P＜0.01)。结论：SC提取物能够预防辐射所致淋巴细胞减少和免疫功能损伤。

关键词: 五味子, 辐射, 免疫功能, 淋巴细胞, 预防

Abstract: OBJECTIVE: To study the effects of Schisandra Chinensis (SC) extract on prevention and treatment of immune dysfunction caused by radiation. METHODS：48 BALB/c mice were divided into 4 groups，SC treated group (SC 4 mg/g + 6 Gy for 396 s)，NS group (NS + 6 Gy for 396 s)，SC control group (SC 4 mg/g）and normal group (NS). WBC and lymphocyte subset(LYM) of peripheral blood were counted. Percentages of CD4+ and CD8+ T cells were measured by flow cytometry. Contents of IgG and C3 in mouse serum were analyzed by immunological turbidimetry. RESULTS：After irradiation，the number of WBC and LYM in peripheral blood of NS-treated mice was lower than that of normal control group [(3.73±1.05)×109 and (2.06±0.57)×109，respectively，P＜0.01]. Compared to NS group，the number of WBC and LYM of SC treated mice was higher [(6.43±0.76)×109 and (4.15±0.95)×109，respectively，P＜0.01]. There was no significant difference between SC group and NS group (P＞0.05). The absolute numbers of CD4+ and CD8+ T subsets and the levels of IgG and C3 were decreased after radiation (P＜0.01). Compared to NS group，the number of T lymphocyte subsets in SC-treated mice was higher than that of NS group (P＜0.01). CONCLUSION：SC could prevent the lymphocyte reduction and immune injury caused by radiation.

Key words: Schisandra Chinensis, radiation, immune function, lymphocyte, prevention

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