关键词: 双酚A, 敌匹硫磷, MCF-7细胞

Abstract: BACKGROUND AND AIM: To study the effects of Bisphenol A (BPA) and/or diazinon on proliferation of MCF-7 cells. MATERIALS AND METHODS: Estrogen-sensitive and insensitive MCF-7 cells were used to evaluate proliferating effects of BPA and diazinon with concentrations of 1.0×10-12～1.0×10-5mol/L (10 times difference between two consecutive groups). Then the change of proliferating effects after using estrogen receptor inhibitor ICI182780 in each group was assessed. solrent control was establishect in the experiments Combined effects of BPA and diazinon on proliferation of MCF-7 at concentrations of 1.0×10-8, 5.0×10-9 and 2.5×10-9mol/L were evaluated by 2×2 factorial analysis. RESULTS: The highest relative proliferation rate of BPA (2.473) and diazinon (2.167) (compared with solvent control,P<0.05) were all at 1.0×10-8mol/L. After adding ICI182780, the proliferating effects of the drugs on MCF-7 declined. The drugs had no obvious proliferating effects on estrogen-insensitive MCF-7 cells. The combined effects of BPA and diazinon at 1.0×10-8, 5.0×10-9 and 2.5×10-9 mol/L were all antagonistic. CONCLUSION: Bisphenon A and diazinon had obvious estrogen-like activity. They exerted proliferating effects by stimulating the estrogen receptor. The combined effect of BPA and diazinon was antagonistic.

Key words: Bisphenol A, diazinon, MCF-7 cells