关键词: 电子垃圾, CYP1A1 MspⅠ, GSTT1, 基因多态性, 双核淋巴细胞微核率

Abstract: BACKGROUND AND AIM: Associations between genetic polymorphisms of CYP1A1 MspⅠor GSTT1 among residents from an electronic waste (e-waste) recycling site in China,and the frequencies of micronucleated binucleated cells (MNed BNC) in peripheral blood lymphocytes were investigated. MATERIALS AND METHODS: We recruited 54 residents from a decade-long e-waste recycling site in southeast China (as exposed group), and another 73 farmers from one village located 50 km away from the e-waste site without other pollutions(as control group). The frequencies of MNed BNC were analyzed by cytokinesis-blocked micronucleus assay. CYP1A1 Msp I genotypes and GSTT1 genotypes were analyzed using polymerase chain reaction -based restriction fragment length polymorphism method and multiple polymerase chain reaction method, respectively. RESULTS: The mean MNed BNC frequency in the exposed group was 4.81‰± 4.14‰, which was approximately four times the mean MNed BNC frequency of the control(1.15‰±1.42‰,P<0.01). After adjusted by age, gender, cigarettes per day and alcohol drinking, we found that the mean frequency of MNed BNC among subjects with homozygous rara allele (aa) genotype at CYP1A1 MspⅠsite was 1.69‰±1.28‰, which was significantly higher than those of subjects with either homozygous wild type or heterozygous genotypes(AA/Aa) (1.06‰±1.44‰, P<0.01)in the control. However, there was no association between the frequency of MNed BNC and GSTT1 genotypes alone or both GSTT1 genotypes and CYP1A1 genotypes. CONCLUSION: The findings suggest that polymorphism of CYP1A1 MspⅠmay be associated with the frequency of MNed BNC among the control subjects. Certain pollutants from the e-waste dismantling site surroundings may affect the health of the local residents.

Key words: e-waste, CYP1A1 MspⅠ, GSTT1, polymorphisms, micronucleated binucleate cells