天元甘露液对小鼠肠道5种微生物菌群定植的调节作用

doi:10.3969/j.issn.1004-616x.2025.02.010

癌变·畸变·突变 ›› 2025, Vol. 37 ›› Issue (2): 153-156.doi: 10.3969/j.issn.1004-616x.2025.02.010

天元甘露液对小鼠肠道5种微生物菌群定植的调节作用

杨玉^1,2, 马越², 李景云², 单纯²

1. 佳木斯大学基础医学院, 黑龙江佳木斯 154007;
2. 中国食品药品检定研究院, 北京 100050

收稿日期:2024-07-26 修回日期:2024-10-10 出版日期:2025-03-30 发布日期:2025-04-11
通讯作者: 单纯
作者简介:杨玉，E-mail:yangyu@jmsu.edu.cn。
基金资助:
黑龙江省卫健委科研课题(20210202040066)；佳木斯大学青年创新人才培养支持计划(JMSUQP2020012)

Regulatory effects of Tianyuan manna on colonization of five intestinal microbial flora in mice

YANG Yu^1,2, MA Yue², LI Jingyun², SHAN Chun²

1. Basic Medical College of Jiamusi University, Jiamusi 154007, Heilongjiang;
2. National Institutes for Food and Drug Control, Beijing 100050, China

Received:2024-07-26 Revised:2024-10-10 Online:2025-03-30 Published:2025-04-11

摘要/Abstract

摘要： 目的：建立小鼠肠道菌群失调模型，观察天元甘露液是否具有对肠道5种微生物菌群定植的调节功能。方法：将50只SPF昆明小鼠随机分为5组，即空白对照组，模型(酸牛奶)对照组，天元甘露液(含酸牛奶)4.2、8.3和16.6 g/kg组，每组10只。连续7 d经口灌胃给予200 mg/kg诺氟沙星造模，造模之后连续16 d灌胃给予天元甘露液或酸牛奶，用浇碟法计数肠道双歧杆菌和乳杆菌(2个有益菌)，产气荚膜梭菌、肠球菌和肠杆菌(3个有害菌)共5种微生物菌群定植活菌数量，并与空白对照组和模型对照组比较。结果：与空白对照组比较，经诺氟沙星连续灌胃给药7 d后，小鼠肠道5种微生物菌群活菌数明显减少(P<0.01)，表明造模成功；连续16 d灌胃给予天元甘露液或酸牛奶后，小鼠肠道上述2种有益菌活菌数均显著增加(P<0.01)，3种有害菌活菌数达到或低于空白对照组(P<0.05或P<0.01)。与模型对照组比较，天元甘露液各剂量组有害菌数量明显减少(P<0.05或P<0.01)，8.3 g/kg组有益菌数量轻度增加(P<0.05)。结论：天元甘露液在肠道菌群失调到菌群平衡过程中具有对本文所述5种微生物菌群定植的调节作用。

关键词: 天元甘露液, 小鼠, 肠道微生物, 菌群定植

Abstract: OBJECTIVE：To establish a mouse model of intestinal microbiota dysbiosis and to observe whether Tianyuan manna and/or yoghurt would regulate colonization of 5 kinds of intestinal microflora.METHODS：The SPF Kunming mice were divided into five groups：blank control group，model (yoghurt) control group，Tianyuan manna (containing yoghurt) 4.2，8.3 and 16.6 g/kg groups，with 10 mice in each group. The model was established by oral administration of 200 mg/kg norfloxacin for 7 consecutive days. After the model was established，Tianyuan manna and/or yoghurt were given by gavage for 16 consecutive days. The number of intestinal bifidobacteria and Lactobacillus (2 beneficial bacteria)，Clostridium perfringens，Enterococcus and Enterobacter (3 harmful bacteria) were counted by pouring dish method，and compared with the blank control group and the model control group. RESULTS：After the norfloxacin treatment，the number of viable intestinal microflora was significantly reduced compared with the blank control group (P<0.01)，indicating that the model was successful. After administration of Tianyuan manna and/or yoghurt，two beneficial bacteria exceeded the number of intestinal viable bacteria in normal mice (P<0.01)，and three harmful bacteria were lower (P<0.05 or P<0.01). Compared with the model control group，the number of harmful bacteria in each dose group of Tianyuan manna was significantly reduced (P<0.05 or P<0.01)，and the number of beneficial bacteria (Bifidobacterium) in the 8.3 g/kg group was slightly reduced (P<0.05). CONCLUSION：In the process balance and imbalance of intestinal flora imbalance，Tianyuan manna demonstrated regulatory effect on the colonization of five kinds of microbial flora.

Key words: Tianyuan manna, mice, gut microbiota, microbiota colonization

中图分类号:

R965

杨玉, 马越, 李景云, 单纯. 天元甘露液对小鼠肠道5种微生物菌群定植的调节作用[J]. 癌变·畸变·突变, 2025, 37(2): 153-156.

YANG Yu, MA Yue, LI Jingyun, SHAN Chun. Regulatory effects of Tianyuan manna on colonization of five intestinal microbial flora in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2025, 37(2): 153-156.

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天元甘露液对小鼠肠道5种微生物菌群定植的调节作用

Regulatory effects of Tianyuan manna on colonization of five intestinal microbial flora in mice

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