关键词: 苦参素, 微核, LD50, 外周血, 精子畸变

Abstract: BACKGROUND ＆ AIM:To investigate the acute toxicity and genotoxicity of Marine in mice. MATERIAL AND METHODS: LD50 test , micronucleus test in peripheral blood PCE and sperm abnormality test were used.The mice were randomly divided into five groups:the negative control(I),the positive control(II),1/2 LD50(III),1/4 LD50(IV),1/8 LD50 (V). III,IV,V groups were administrated with different dosages of Marine intraperitoneally. RESULTS： The LD50 of the Marine was (505±31)mg/kg. Compared to the negative control, the results showed that 1/2 LD50,1/4 LD50,1/8LD50 groups of 24 hours micronucleated PCE could significantly increase the micronucleus frequencies in the peripheral blood. The same condition could be observed in 1/2 LD50,1/4 LD50,1/8 LD50 groups of 48 hours micronucleated PCE.1/2 LD50 group differed from the negative control in the sperm abnormality test, whereas no significant difference has been observed between 1/4 LD50,1/8 LD50 groups and negative control. CONCLUSION： It suggests that the Marine is a low toxicity drug and has genotoxicity to a certain extent.

Key words: Marine, LD50, peripheral blood, micronucleus, sperm abnormality