HeLa - MLH1 - 细胞的生物学特性检测

doi:10.3969/j.issn.1004-616x.1998.04.003

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (4): 203-206.doi: 10.3969/j.issn.1004-616x.1998.04.003

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THE DETECTION OF BIOLOGICAL CHARACTERISTICS OF HELA2MLH1 - CELL

Zhang Yun , Qian Ying , Yu Yingnian , Wang Aijin , Luo J ianhong

Department of Pathophysiology Zhejiang Medical University and Provincial key L aboratory of Medical Molecular Biology , Hangz hou 310031

Received:1900-01-01 Revised:1900-01-01 Online:1998-07-30 Published:1998-07-30

Abstract

Abstract: Spontaneous mutation rate at hypoxanthine phosphoribosylt ransferase (hprt) locus increased markedly in Hela2MLH1 - cell , mismatch repair (MMR) deficient cell line , it exhibited a 8. 78 and 3. 532fold increase in mutation f requency relative to MMR2proficient HeLa2R9 and HeLa cell line. This phenotype is similar to another mismatch repair defective cell line HeLa2MSH2 - .MNN G tolerance also appeared in HeLa2MLH1 - cell but no MNN G tolerance in HeLa2MSH2 -cell. These data suggest that different MMR gene defect exert s different effect s on mutator phenotypes.

Key words: DNA2repair, hypoxanthine phosphoribosylt ransferase, mutation, methyl-nitro-ni-t rosoguanidine

Zhang Yun , Qian Ying , Yu Yingnian , Wang Aijin , Luo J ianhong. THE DETECTION OF BIOLOGICAL CHARACTERISTICS OF HELA2MLH1 - CELL[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1998, 10(4): 203-206.

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THE DETECTION OF BIOLOGICAL CHARACTERISTICS OF HELA2MLH1 - CELL

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