Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (4): 243-247.doi: 10.3969/j.issn.1004-616x.1998.04.014

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0STUDY OF TEXTING METHOD OF EXPRESSION OF P53 GENE MUTATION AND MUTATION PROTEIN IN EPITHELLIAL OVARIAN TUMOR

J in Zhen , Li Shourou , Huang Bingyu ,Leng Weichun , Zhang Aichen   

  1. Ob & Gyn . Dept , Second Clinical Hospital of China Medical University S henyang ,110003
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-07-30 Published:1998-07-30

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Abstract: To dectect P53gene mutation and it s mutation protein ,improved aPCR - SSCP silver staining and SP immunohistochemical methods were emploied in 12 borderline epithelial ovarian tumor and 15 benign epithelial ovarian tumor. The result s showed that positive rate of P53 gene mutation was 30. 6 % , and that of the mutation protein was 58. 4 %. The mutation of P53 gene was mainly on it s exon 5 - 8 ,especially on exon 7. In some cases of borderline tumor , P53 gene and it s mutation protein were positive , but nine in benign tumor This indicated that P53gene mutation was a later event in the form ovarian carcinoma. P53gene mutation might serve as a genetic marker to determine the prognosis of ovairan carcinoma. aPCR - SSCP sliver staining method overed the defect of former method ,and improved the accuracy of esamine.

Key words: Epithelial ovarian tumor, Gene P53, Mutation