Carcinogenesis, Teratogenesis & Mutagenesis ›› 2000, Vol. 12 ›› Issue (3): 129-132.doi: 10.3969/j.issn.1004-616x.2000.03.001

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CLONING OF THE HISTONE DEACETYLASE 2 cDNA AND IT’S EXPRESSION IN YEAST

CHEN Jian1 , ZHAN G Xiao-qin2 , FU Ji-liang3   

  1. 1. Department of Naval Hygiene , 2. Inf ection Department Changhai Hospital , 3. Instit ute of Medical Biotechnology Molecular Genetics , Second Military Medical University , S hanghai , 200433 , China
  • Received:2000-01-13 Revised:2000-02-25 Online:2000-07-30 Published:2000-07-30

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Abstract: Purpose : In order to obtain histone deacetylase 2 (HDAC2) cDNA and const ruct the target gene of yeast two hybrid system. Methods : we amplified about 1. 5 Kb DNA f ragment f rom HeLa cell using RT - PCR method. After automatic sequeced , a recombinant plasmid pLexA - HDAC2 was made and t ransformed into yeast EGY48 (p8op - Lac2) , and the expression of pLexA - HDAC2 in the yeast was observed. Results : Results show that : The sequence of RT - PCR product was same with the sequence of reported HDAC2 cDNA. About 1mm white yeast clone was grown in the selective medium after 3 days. Conclusion : The result s indicated that : HDAC2 cDNA has been obtained. pLexA - HDAC2 can be expressed in yeast with inactivation of LacZ gene and nontoxic to yeast , and can be as a target gene of yeast two hybrid system.

Key words: histone deacetylation, histone deacetylase2 (HDAC2), yeast two hybrid system

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