Purpose : In order to obtain histone deacetylase 2 (HDAC2) cDNA and const ruct the target gene of yeast two hybrid system. Methods : we amplified about 1. 5 Kb DNA f ragment f rom HeLa cell using RT - PCR method. After automatic sequeced , a recombinant plasmid pLexA - HDAC2 was made and t ransformed into yeast EGY48 (p8op - Lac2) , and the expression of pLexA - HDAC2 in the yeast was observed. Results : Results show that : The sequence of RT - PCR product was same with the sequence of reported HDAC2 cDNA. About 1mm white yeast clone was grown in the selective medium after 3 days. Conclusion : The result s indicated that : HDAC2 cDNA has been obtained. pLexA - HDAC2 can be expressed in yeast with inactivation of LacZ gene and nontoxic to yeast , and can be as a target gene of yeast two hybrid system.

Purpose and Methods : To explore the genetic toxicity of NaF , we used PCR - SSCP and immunohistochemist ry methods to detect the effect s of NaF on p16 gene and P16 protein of human embryonic lung fibroblast (HEL F) cells. Results and Conclusion : The result s show that NaF can’t induce the change of p16 gene.

Purpose : In order to study the effects of trans - BPDE on the development in human lung cancer , human fetal tracheal epithelium cells (HFTEs) cultured in vit ro were exposed to t rans - BPDE , a metabolite of benzo (a) pyreneB (a) P . Methods : For 8～12 weeks , the point mutations in exon 6～8 of p53 tumor suppressor gene in these HFTEs were detected by PCR - SSCP assay. Results : The results showed that the HFTEst reated with t rans - BPDE had point mutations in exons 7 and 8 of p53 tumor suppressor gene , even though no malignantly t ransformed characteristics were observed in cellular morphology. Conclusion : these result ssuggest that t rans - BPDE can induce point mutation in p53 tumor suppressor gene in these HFTEs and the point mutation and activation of p53 may occur in the cells before morphological t ransformation.

Abstract : Purpose : To study the expression of TNF -αmRNA and TNF -αprotein in the development from hyperplasia , dysplasia to carcinoma of gallbladder mucosa and to discuss the pathogenesis of gallbladder mucosa and to discuss the pathogenesis of gallbladder carcinoma. Methods : In situ hybridization and immunohistochemist ry were used to detect the expression of TNF -αmRNA , TNF -αprotein in mucosa of hyperplasia , dysplasia and carcinoma of gallbladder. Results : ①The percentages of positive cells and the numbers of positive cells/per high power field (HPF) which expressed TNF - αmRNA in hyperplasia , dysplasia and carcinoma of gallbladder mucosa cell and mononuclear cells(MNC) were increasing. ②The numbers of carcinoma cells and MNC/ HPF which expressed TNF -αmRNA increased with increasing tumor grades and size. ③The pattern of TNF -αprotein expression was similar to that of TNF -αmRNA , but more f requent and widespread. ④There was a positive linear correlation between tumor cells and MNC of TNF -αmRNA and TNF -αexpression. Conclusion : TNF -αwas involved in the pathogenesis of gallbladder carcinoma induced by gallstone and the TNF -α expression in cancer cells may possibly be a marker for tumor staging.

Purpose and Methods : The ob gene point mutant in C57BL/ 6J ob mice cause genetic obesity in all life. To improve the speed and efficiency of ob genotyping , we developed a method with PCR and rest riction endonuclease by property primer. Results and Conclusion : Three genotype (ob/ ob , ob/ + , + / + ) of the mouse are rapidly , differetiated with this assay. Since only a small biopsy specimen ( tail end) is needed to genotype ,the ob mouse at any age is typable and can be used in subsequent breeding or research.

Purpose and Methods : By using mouse whole embryo culture technique , the in vit ro model of cyclophosphamide - induced teratogenicity was established. The cellular effects of cyclophosphamide on mouse embryos was evaluated by light microscopy and flow cytomet ry. Results and Conclusion : The result s suggested that the variation of cell cycle and the occurrence of apoptosis might be involved in cyclophosphamide - induced teratogenicity.

Purpose : In order to observe the effect of chemotherapeutic drugs and Zheng's plant - protein on 100 case human solid tumor cells and to obtain information of tumor cell growing and of select sensitive chemotherapeutic drugs. Methods : The activity of sample tumor cell in primary culture was detemined with MTT colorimet ry. Results and Conclusion : The proliferation of 30 % sample tumor was unwell , but the other 70 % was well.Result s showed high sensitivity on sample tumor cell are cisplatin , carboplatinum , 5 - Fluorouracil , mitomycin and bleomycin. The proliferation of the tumor cells was promoted by stimulation of Zheng’s plant - protein .The proliferation of a few sample tumor cells was promoted by incristine , adriamycin and vepeside also.

Purpose and Methods : By Ames , micronucleus test on mouse bone marrow polychromatic erythrocytes (PCE) and fish peripheral blood erythrocyte the mutagenicity of concent rated substance with XAD - 2 big hole resin is studied in the out2factory water ,tip water ,cement chamber water ,boiling water and source water. Results and Conclusion : The result s showed that the concent rated substances of all samples have mutagenic effect . The mutagenic active size is in proper order out - factory water ,boiling water ,tip water ,cement chamber ater ,source water.

Purpose : To study the genotoxicity of non - volatile organic chemicals in Huai river water to organism. Methods : zool of water sample was collecfed f rom Huai river source water、clarified filt ration water 、chlorinated tap water and reservoir’s water , respectively. After being adsorpted and concent rated , water samples were administ rated by gavage into mouse stomach. The mutagenicity was studied by means of micronucleus test in mouse thoracic bone - marrow polychromatic erythrocyte (PCE) and the genotoxicity by means of sperm morphologic analysis. Results : the mutagenicity and sperm deformity of some degree are induced by Huai river source water and tap water. The order of mutagenic potentiality of those samples is as follows : chlouinated tap water > reservoir’s water > source water > clarifying fillration water. Conclusion : The source water is contaminated by organic mutagens. Using coagulation and precipitation , genotoxicity of source water is decreased because of the removal of some mutagenic organic chemicals. Addifionally chlorination can increase the genotoxicity of tap water.

Purpose and Methods : Mutagenicity , teratogenicity and subchronic toxicity in mice or rat s induced by Methylamino2Abamectin (MA) were studied. Results and Conclusion : In comparison with negative cont rol , each dosage did not show significant differences in the rates of marrow cell micronuclei and spermatic deformity of mice ( P > 0. 05) ; but revealed significant difference compared to positive cont rol ( P < 0. 05) . MA (102500μg/ plate) didn’t induce positive mutations of st rains TA97 ,TA98 ,TA100 and TA102 with or without S9mix n Ames test . It suggested that MA has no obvious mutagenicity and embryotoxic or teratogenic effects in Wistar rats. At the highest dosage , BUN and Albumin at 12. 6mg/ kg. bw in females showed significant diffence ( P <0. 05) , so did BUN at 9. 26mg/ kg. bw in males ( P < 0. 05) . Body weight s were decreased in males and organ coefficient of kidney was increased in males ad females. The maximum no2observed2effect level of MA in subchromic toxicity was 1. 16mg/ kg. bw and 1. 57mg/ kg. bw in males and females , respectively.

Purpose and Methods : The acute toxic and mutagenic effect s of acrosin inhibitor KF - 950 were evaluated in mice and Ames assay. Results and Conclusion : Half lethal dosage of KF - 950 in mice was more than 5 000mg/ kg. The mutagenic activity of KF - 950 , under the doses used , was not observed in TA97 , TA98 , TA100 ,TA102 ,with or without S9 activitor.

Purpose and Methods :By Ames test , micronucleus test and sperm diformity test , the genetic toxicity was studied for mice exposed t raffic contaminated road in XIAN G YA , BAN ZAN , SON G GUI. Results and conclusion : The result s showed that there are direct and indirect mutagens in the lung tissue of contaminated mice , that the micronucleus rate of chest bone polychomatoerythrocyte ( PCE) and sperm deformity f requencies in contaminated groups were higher than cont rol group. The t raffic pollutant had genetic toxicity effect on the somatic cell and germ cell in the mice

Purpose : To research the teratogenic effect of recombinant staphylokinase ( r - SAK) on ICR mice. Methods : The animals were divided into negative and positive cont rol and three experiment groups which were int ravenously given by r - SAKf rom Day 6 to Day 15 of gestation in dosages of 100mg/ kg. bw , 31. 6mg/ kg. bw and 10mg/ kg. bw , respectively. The body growth gains of gestation mice , the numbers , body weight , length and tail length in living fetus and some indicators of embryonic formation were measured. Results : There were no significant differences as compared with negative cont rol groups. Besides , the deformity of the appearances , internal organs and bone in fetal mice was not found. Conclusion : The result s indicated that r - SAK had no teratogenic effect on ICR mice in this experiment .