Carcinogenesis, Teratogenesis & Mutagenesis ›› 2003, Vol. 15 ›› Issue (3): 144-146.doi: 10.3969/j.issn.1004-616x.2003.03.005

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MEASUREMENT OF DNA DAMAGES INDUCED BY RADIATION AND CHEMICALS USING COMET ASSAY

CAO Yi, CHEN Rui, WANG Zhong-wen, et al   

  1. China Institute for Radiation Protection,Taiyuan 030006, China
  • Received:2002-10-24 Revised:2002-12-15 Online:2003-07-30 Published:2003-07-30
  • Contact: CAO Yi

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Abstract: Purpose: To study DNA damages induced by 60 Co γ-radiation and o-phenylenediamine(o-PDA) using comet assay. Methods: The DNA damages induced by 0,2,4,6,8,10,15 Gy 60 Co γ-rays and 0,2,4,6,8 μmol / L o-PDA were measured using comet assay. The dose-respose relationship of the CHL cell tail length and the dose of the two type of mutagen were established with Origin 4 software. Results: The tail length of comet, index of DNA damage in this experiment， increased with the dose of 60 Co γ-ray and concentration of o-PDA, respectively. The dose-respose relationship of the CHL cell tail length(TL)and the dose of 60 Coγ-ray fitted for linear-quadratic model: TL=20.41＋2.42D＋0.38D 2; The dose-response relationships for DNA damage induced by o-PDA fitted for linear-quadratic model: TL=1.90＋1.46C＋0.52C 2. Conclusion: The comet assay is a useful tool for determining the genotoxicity of environmental agents.

Key words: comet assay, o-phenylenediamine, 60 Co γ-rays, DNA damages