Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (1): 33-35.doi: 10.3969/j.issn.1004-616x.2005.01.011

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Detection of DNA Repair Gene: ERCC2/XPD exon 6 Mutation from Human by Nested Polymerase Chain Reaction (NPCR)

GUO Li; MA Ye-gang;SUN Zhong-fu; et al   

  1. The Center Laboratory, Shenyang Medical College,Shenyang 110034,China
  • Received:2004-06-01 Revised:2004-09-15 Online:2005-01-30 Published:2005-01-30
  • Contact: YIN Jiao-yang

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Abstract: BACKGROUND ＆ AIM: To study a method which can get ideal products of PCR in the sample of low yield DNA for mutation analysis. MATERIAL AND METHODS: The nested polymerase chain reaction(NPCR)-restriction fragment length polymorphisms (RFLP) technique was used for mutation test of single nucleotide polymorphism of DNA repair gene: ERCC2/XPD exon 6 in some samples of low yield DNA.RESULTS: Genotyping results of population were in agreement with the expectations of Hardy-Weinberg Equilibrium. CONCLUSION: The sophisticated new“nested PCR” systems are significantly more sensitive and specific than other currently availble PCR technologies. 　

Key words: nested-polymerase chain reaction, low yield DNA, DNA repair gene: ERCC2/XPD exon 6