Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (2): 93-098.doi: 10.3969/j.issn.1004-616x.2007.02.003

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Combined Effects of PCB126 and B(a)P on Genotoxicity of HepG2 Cells by Its Metabolic Enzyme Changes

ZHANG Chi，LIN Hui, WEI Wei, LIU Ai_lin, CHEN Xue_min, LU Wen_qing   

  1. Department of Occupational and Environmental Health, MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,Hubei,China
  • Received:2006-09-27 Revised:2006-12-05 Online:2007-03-30 Published:2007-03-30
  • Contact: LU Wen_qing

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Abstract: BACKGROUND ＆ AIM: To study effects of polychlorinated biphenyl PCB126 on B(a)P_induced genotoxicity. MATERIALS AND METHODS: HepG2 cells were treated either with different concentrations of PCB126 (0.01, 0.10,1.00 nmol/L) alone,or with different concentrations of PCB126 for 48 h then with B(a)P (50 μmol/L) and PCB126 together for another 24 h. DMSO and 3_MC were used as solvent control and positive control, respectively. EROD activity and micronuclei(MN) formation were analyzed through fluorescence spectrophotometry and a cytokinesis_block micronucleus(CBMN) assay, respectively. The frequencies of MN(‰) and nuclei division index (NDI) were calculated. RESULTS: Comparing to solvent control, EROD levels increased markedly in HepG2 cells treated with PCB126 (0.01, 0.10, 1.00 nmol/L) and B(a)P (50 μmol/L) alone or together, and MN frequencies increased significantly only in HepG2 cells treated with B(a)P alone(P<0.05,P<0.01). Comparing to B(a)P treatment alone, the EROD levels and MN frequencies increased significantly in HepG2 cells treated with B(a)P and PCB126 (0.10,1.00 nmol/L) together(P<0.05,P<0.01). CONCLUSION: PCB126 at certain concentrations showed no genotoxic effect in HepG2 cells, but it might enhance the genotoxic properties of B(a)P.

Key words: PCB126, B(a)P, combined effect, micronuclei, CYP1A1

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