Abstract: BACKGROUND AND AIM: To study the effect of oxidative damage in endothelial dysfunction induced by homocysteine and to explore the protective effects of Genistein. MATERIALS AND METHODS: MTT assay was used to detect cell viability, and intracellular reactive oxygen species(ROS) level was measured by flow cytometry(FCM). RESULTS: The cellular viability of 5.0 mmol/ L HCY(41.27％±5.09％) were lower than the others. After co-treatment with 5.0 mmol/L HCY and different concentrations GEN(10,50,100 μmol/L), the cellular viability of each group was elevated. The level of ROS after 5.0 mmol/L HCY treatment increased obviously compared with control group(P<0.01). Co-treatment with 5.0 mmol/L HCY and different concentrations of GEN, the level of ROS was decreased. CONCLUSION: HCY could lead to endothelial dysfunction in ECV-304 through oxidative damage. GEN could protect cell viability and decreased intracellular reactive oxygen species.

Key words: genistein, homocysteine, ECV-304 cells, ROS