荧光原位杂交和CREST 染色对甲基丙烯酸环氧丙酯的非整倍体诱发效应的研究

doi:10.3969/j.issn.1004-616x.1998.02.001

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (2): 65-068.doi: 10.3969/j.issn.1004-616x.1998.02.001

STUDY OF THE ANEUPLOID EFFECT OF GLYCIDYL METHACRYLATE ( GMA) USING FLUORESCENCE IN SITU HYBRIDIZATION AND CREST STAINING

Hu Bin2 , Cao J ia2 , Cheng Ge3 , Cheng Tian Min2

1Department of Heal th Toxicology and Occupational Heal th , S un Yat2Sen University of Medical Sciences 　Guang z hou 　510089 　2 Department of Preventive Medicine , Thi rd Military Medical University , Chong qing 　630038 　3 Department of Biology , Guang Zhou Teachers College , Guangz hou 　510040

Received:1900-01-01 Revised:1900-01-01 Online:1998-03-30 Published:1998-03-30

Abstract

Abstract: In this research Fluorescence in situ hybridization ( FISH) and antikinetochore antibodies (CREST Syndrome serum) were used in determining the effect of inducing aneuploid of GMA. The two techniques mainly detected the proportion of cent ric signals in micronuclei induced by GMA. The result s showed that FISH positive ratio of GMA was 40. 9 - 51. 5 % , but for CREST2staining it was only 17. 0 - 24. 1 %. It indicated that GMA was a suspected spindle poison and could damage the cent ric protein. The metaphase analysis demonst rated that GMA had significantly clastogenic effect , which mostly happened in cent romere region.

Key words: micronuclei, glycidyl methacrylate, Fluorescence in situhybridization, antikinetochore antibodies, aneuploid effect

Hu Bin , Cao J ia , Cheng Ge , Cheng Tian Min. STUDY OF THE ANEUPLOID EFFECT OF GLYCIDYL METHACRYLATE ( GMA) USING FLUORESCENCE IN SITU HYBRIDIZATION AND CREST STAINING[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1998, 10(2): 65-068.

[1]	LI Xinwei, WANG Quankai, MA Shunpeng, WANG Miao, WUHAN Baolier, GU Yiting, KANG Tongying, XU Jianning. Long non-coding RNA expression analysis and ceRNA regulatory network in glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 1-6.
[2]	WANG Miao, WANG Quankai, LI Xinwei, MA Shunpeng, WUHAN Baolier, XU Jianning. m⁶A methylation of mRNAs in glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 405-409.
[3]	MA Shunpeng, WANG Quankai, WANG Miao, SONG Jiayang, WUHAN Baolier, XIE Guangyun, XU Jianning. Long non-coding RNA regulates apoptosis during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 1-5,31.
[4]	XIE Guangyun, GUO Haoran, WANG Quankai, MA Shunpeng, SONG Jiayang, WUHAN Baolier, XU Jianning. Differential expressions of LINC00472 during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 449-453.
[5]	WANG Quankai, XIE Guangyun, MA Shunpeng, GUO Haoran, WUHAN Baolier, SONG Jiayang, XU Jianning. Genotoxicity evaluation of glycidyl methacrylate [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 479-482.
[6]	TANG Jiao, YANG Ying, LI Qing, ZHAO Min, YANG Yingfen. In vitro induction of micronuclei by Panax notoginseng extracting solution [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 397-400.
[7]	WANG Quankai, WANG Boshen, XIE Guangyun, KANG Tongying, ZHU Baoli, XU Jianning. Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 422-426.
[8]	LIU Hongmei, WANG Quankai, XIE Guangyun, WEN Yanan, XU Jianning. Induction of LncRNA PCA3 by glycidyl methacrylate in malignant transformed 16HBE cells and its significance [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 185-189.
[9]	WANG Quankai, XIE Guangyun, LIU Hongmei, XU Jianning. METTL9 methylation status in malignant transformation of 16HBE cells induced by glycidyl methacrylate [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 432-436.
[10]	LI Huan-huan1，WANG An-na1，WANG Quan-kai，TAN Feng，XU Jian-ning. Study on the anti-oncogene of P15 methylation status in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(1): 18-21.
[11]	WANG An-na，DONG Lin，WANG Quan-kai，HU Jie，XU Jian-ning. Changes of TGF-β signal pathway in the process of 16HBE malignant transformation induced by glycidyl methacrylate [J]. , 2012, 24(2): 91-95.
[12]	LIAO Jing，LIU Ai-lin，CAO Wen-cheng，LU Wen-qing. Genotoxicity detection of trihalomethanes and haloaceto-nitriles using cytokinesis-block micronucleus test [J]. , 2012, 24(1): 42-45.
[13]	LI Ying1，WANG Quan-kai1，2，WANG An-na1，2，HU Jie1，XU Jian-ning1，2，*. Expressing the alteration of DNA repair genes in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate [J]. , 2011, 23(3): 161-165.
[14]	LI Xin，CHEN Mei-fen，CHEN Xiu-juan，XIONG Xi-kun，LI Qing，WANG Feng-yan. Safety assessment of Sauropus androgynus L. Merr. in vitro [J]. , 2011, 23(3): 222-225.
[15]	HU Jie, WANG Quan-kai, DONG-Lin, SUN Jin-xiu, XIE Guang-yun, WANG Jian , XU Jian-ning. Altered expressions of DNMT1 and MBD1 genes in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(5): 339-342.

STUDY OF THE ANEUPLOID EFFECT OF GLYCIDYL METHACRYLATE ( GMA) USING FLUORESCENCE IN SITU HYBRIDIZATION AND CREST STAINING

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments