Abstract:

 OBJECTIVE: To add data about mutagenicity of 2-ethylhexyl acrylate(2-EHA)，so as to assess its safety. METHODS：Three genotoxicity tests，bacterial reverse mutation assay (Ames test)，micronucleus test of bone morrow cells and TK gene mutation assay with cell line L5178Y were performed in this study. Doses were designed at 0.08，0.4，2.0，10.0，50.0 μL/palte of Ames test，at 350，700，1 400 mg/kg of micronucleus test and at 12.5，25，50，100 μmol/L of TK gene mutation assay. RESULTS：In Ames test，2-EHA showed mutagenicity in Salmonella typhimurium TA97，TA98，TA100 and TA102 strains with or without S9. The micronucleus rate of the highest dose group in male mice (1 400 mg/kg) was significant increased compared with control group，while it was slightly increased in female mice. In TK gene mutation assay，the mutation frequency in two highest dose groups (50 and 100 μmol/L) were significantly higher than that in control group. CONCLUSION：Under present experiental conditions，2-EHA showed genotoxicity in Ames test and TK gene mutation assay，micronucleus test were probably positive for 2-EHA. Genotoxicity of 2-EHA should be considered comprehensively by other mutagenicity tests.

Key words: 2-ethylhexyl acrylate, Ames test, micronucleus test, TK gene mutation assay