应用冰冻切片标本检测乳腺癌c - erbB- 2 基因扩增的研究

doi:10.3969/j.issn.1004-616x.2000.01.015

Abstract

Abstract: Purpose : The amplification of HER - 2/ neu may have prognostic value in breast cancer. Methods : In this study , we chose the reference gene on the same chromosome as the target gene , and used the differential polymerase chain reaction (d - PCR) to assess the presence of c - erbB - 2 gene amplification in frozen sections of breast cancer. Results : The result s showed that there were 19 of 71 cases of breast cancer were detected amplification of c - erbB - 2 gene , the detectable rate was 26. 7 % , which was higher than our previous reports. Conclusion : the author regards that the chose of primer and the specimens cont ribute to the improvement of the detectable rate.

Key words: differential polymerase chain reaction (d - PCR), breast cancer, c - erbB - 2 gene

CLC Number:

R737. 904. 5

WU Jian-zhong, SHEN Zong-li, CHEN Sen-qing, XUE Kai-xian. STUDY ON C - ERBB - 2 GENE AMPLIFICATION IN FROZEN SECTIONS OF BREAST CANCER[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2000, 12(1): 47-048.

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STUDY ON C - ERBB - 2 GENE AMPLIFICATION IN FROZEN SECTIONS OF BREAST CANCER

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