癌变·畸变·突变 ›› 1998, Vol. 10 ›› Issue (3): 187-189.doi: 10.3969/j.issn.1004-616x.1998.03.018

• 技术与方法 • 上一篇    下一篇

一种快速显示抗突变作用机理的试验设计

赵泽贞 温登瑰 魏丽珍 支惠英   

  1. 河北省肿瘤研究所 石家庄 050011
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-05-30 发布日期:1998-05-30

EXPERIMENT DESIGNING FOR RAPID ASSESSMENT OF THE ROLE OF AN ANTIMUTAGEN

Zhao Zezhen ,Wen Denggui ,Wei Lizhen and Zhi Huiying   

  1. Hebei Cancer Instit ute , S hi jia z huang 050011 P. R . China .
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-05-30 Published:1998-05-30

摘要: 为建立一种在检测物质的抗突变性的同时能显示出其作用机理的快速试验方法,我们做了一种试验设计,预期能揭示出抗突变物是直接灭活致突变剂的细胞外作用还是细胞内的抗突变作用,并区分是保护细胞免受损伤还是促使已突变的细胞DNA 修复,或具有多种综合作用。该设计以检测已知抗突变剂VitC 为例效果良好。

关键词: 抗突变, 机理, 试验设计

Abstract: To distinct the mechanism of an antimutagen with SOS inductest ,the authors designed a series of lab procedures which could reveal whether the antimutagen deactivate a given mutagen ext racellularly or protect the targer cell against mutagenesis ext racellularly ,whether the antimutagen interrupt mutagensis int racellularly or has an ability to repair damage caused by the mutagen. After using Vitamin C as a standard antimutagen ,we found that the lab procedures could be put into practice effectively.

Key words: Antimutagenicity, Mechanism, Lab designing